{"id":8605,"date":"2025-05-03T15:59:30","date_gmt":"2025-05-03T06:59:30","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8605"},"modified":"2025-07-14T08:22:46","modified_gmt":"2025-07-13T23:22:46","slug":"calcium01en","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/biosensor01en\/calcium01en","title":{"rendered":"Calcium Ion Sensor"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>Calcium-ion sensor clones<\/h2>\n<p>Fluorescent protein-based Ca<sup>2+<\/sup> sensors, which are composed with calmodulin, fluorescent protein and M13 peptide (CaM binding domain of myosin light chain kinase), are designated to visualize intracellular [Ca<sup>2+<\/sup>] dynamics.<br \/>\nVarious improvements of the Ca<sup>2+<\/sup> sensors have been made for optimum temperature, signal\/noise ratio, susceptibility and so on.<br \/>\nMany papers using Ca<sup>2+<\/sup> sensors have been published reporting the estimation of calcium concentration. <\/p>\n<h3>G-CaMP and R-CaMP<\/h3>\n<table cellspacing=\"0\" cellpadding=\"5\">\n<tbody>\n<tr valign=\"top\">\n<td class=\"t02\" style=\"text-align: center;\"><strong>probe name<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>Ca<sup>2+<\/sup>affinity (K<sub>d<\/sub>, nM)<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>dynamic range (F<sub>max<\/sub>\/F<sub>min<\/sub>)<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>features&amp;application<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>refference<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>plasmid clone<br \/>\n(cat. num)<\/strong><\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">G-CaMP1.6<\/td>\n<td class=\"t02\">146<\/td>\n<td class=\"t02\">4.9<\/td>\n<td class=\"t02\">Brightness improved version. Work at 25<sup>o<\/sup>C (hippocampal slice of rat) to 28<sup>o<\/sup>C (HEK293 cell, HeLa cell) but not at 37<sup>o<\/sup>C (HEK293 cell).<\/td>\n<td class=\"t02\">PMID 11175727, 16159115, 16537386<\/td>\n<td class=\"t02\">pN1-G-Camp1.6 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06749\">RDB06749<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">G-CaMP2<\/td>\n<td class=\"t02\">146<\/td>\n<td class=\"t02\">3.9 to 4.9<\/td>\n<td class=\"t02\">Heat stable version. Work at 37<sup>o<\/sup>C (HEK293 cell, heart of mouse embryo, primary culture of hippocampal astrocytes).<\/td>\n<td class=\"t02\">PMID 16537386, 22472649<\/td>\n<td class=\"t02\">pN1-G-CaMP2 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06750\">RDB06750<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">G-CaMP-HS<\/td>\n<td class=\"t02\">102<\/td>\n<td class=\"t02\">4.1<\/td>\n<td class=\"t02\">High-Ca<sup>2+<\/sup> affinity version (ie: mammalian cell, zebrafish).<\/td>\n<td class=\"t02\">PMID 21383146, 27239939<\/td>\n<td class=\"t02\">G-CaMP-HS (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14607\">RDB14607<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">G-CaMP6<\/td>\n<td class=\"t02\">158<\/td>\n<td class=\"t02\">11.4 to 27.9<\/td>\n<td class=\"t02\">Sensitivity improved version. Quick ON\/OFF response allow to observe neuronal-cell calcium spikes (ie: mouse brain section, mouse B cell, C. elegans).<\/td>\n<td class=\"t02\">PMID 23240011, 27385517, 27428827<\/td>\n<td class=\"t02\">G-CaMP6 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14609\">RDB14609<\/a>)<br \/>\npMXs-IRES-hNGFR_G-CaMP6 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14513\">RDB14513<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">G-CaMP7.09<\/td>\n<td class=\"t02\">212<\/td>\n<td class=\"t02\">19.3<\/td>\n<td class=\"t02\">Enhanced brightness allow to observe flashing fluorescence of iPS cell-derived cardiomyocytes of cynomolgus monkey.<\/td>\n<td class=\"t02\">PMID 27723741<\/td>\n<td class=\"t02\">G-CaMP7.09 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14612\">RDB14612<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">G-CaMP9a<\/td>\n<td class=\"t02\">59<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">Fast kinetics and a high signal-to-noise ratio (ie: tg-mouse).<\/td>\n<td class=\"t02\">Cell Reports Methods 2: 100168 (2022)<\/td>\n<td class=\"t02\">G-CaMP9a (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19172\">RDB19172<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">R-CaMP1.07<\/td>\n<td class=\"t02\">192<\/td>\n<td class=\"t02\">14.4 to 28.7<\/td>\n<td class=\"t02\">CaMP with a red fluorescent protein.<\/td>\n<td class=\"t02\">PMID 22808076, 25419959<\/td>\n<td class=\"t02\">R-CaMP1.07 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14608\">RDB14608<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">R-CaMP2<\/td>\n<td class=\"t02\">69<\/td>\n<td class=\"t02\">4.8<\/td>\n<td class=\"t02\">High affinity Ca\/CaM sensor (ie: cultured hippocampal neurons).<\/td>\n<td class=\"t02\">PMID 25419959<\/td>\n<td class=\"t02\">pBS-CaMKIIpro-R-CaMP2-WPRE (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19475\">RDB19475<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">RCaMP3<\/td>\n<td class=\"t02\">101<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">PMID 38514778<\/td>\n<td class=\"t02\">pAAV-eSyn-RCaMP3-WPRE (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19694\">RDB19694<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">XCaMP-R<\/td>\n<td class=\"t02\">97<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">One of a series of multicolor calcium sensors XCaMP<\/td>\n<td class=\"t02\">PMID 31080068<\/td>\n<td class=\"t02\">pBS-CaMKIIpro-XCaMP-R-WPRE (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19474\">RDB19474<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">XCaMP-G<\/td>\n<td class=\"t02\">200<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">One of a series of multicolor calcium sensors XCaMP<\/td>\n<td class=\"t02\">PMID 31080068<\/td>\n<td class=\"t02\">pBS-CaMKIIpro-XCaMP-G-WPRE (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19471\">RDB19471<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">XCaMP-Gf<\/td>\n<td class=\"t02\">128<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">One of a series of multicolor calcium sensors XCaMP<\/td>\n<td class=\"t02\">PMID 31080068<\/td>\n<td class=\"t02\">pBS-CaMKIIpro-XCaMP-Gf-WPRE (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19470\">RDB19470<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">XCaMP-Y<\/td>\n<td class=\"t02\">81<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">One of a series of multicolor calcium sensors XCaMP<\/td>\n<td class=\"t02\">PMID 31080068<\/td>\n<td class=\"t02\">pBS-CaMKIIpro-XCaMP-Y-WPRE (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19472\">RDB19472<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">XCaMP-B<\/td>\n<td class=\"t02\">71<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">One of a series of multicolor calcium sensors XCaMP<\/td>\n<td class=\"t02\">PMID 31080068<\/td>\n<td class=\"t02\">pBS-CaMKIIpro-XCaMP-B-WPRE (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19473\">RDB19473<\/a>)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Yellow Cameleon<\/h3>\n<\/tr>\n<table cellspacing=\"0\" cellpadding=\"5\">\n<tbody>\n<tr valign=\"top\">\n<td class=\"t02\" style=\"text-align: center;\"><strong>probe name<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>Ca<sup>2+<\/sup>affinity (K<sub>d<\/sub>, nM)<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>dynamic range (F<sub>max<\/sub>\/F<sub>min<\/sub>)<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>features&amp;application<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>refference<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>plasmid clone<br \/>\n(cat. num)<\/strong><\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">YC2.12 Yellow cameleon<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">Ca<sup>2+<\/sup> indicator based on calmodulin and FRET by CFP-Venus pair (ie. Purkinje neuron of mouse cerebellar slice).<\/td>\n<td class=\"t02\">PMID 11753368, 15247428<\/td>\n<td class=\"t02\">YC2.12\/pCS2 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15132\">RDB15132<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">YC3.12 Yellow cameleon<\/td>\n<td class=\"t02\">1,500<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">Low-affinity Ca<sup>2+<\/sup> indicator based on calmodulin and FRET by CFP-Venus pair. <\/td>\n<td class=\"t02\">PMID 15247428<\/td>\n<td class=\"t02\">YC3.12\/pCS2 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15133\">RDB15133<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">YC2.60 Yellow cameleon<\/td>\n<td class=\"t02\">40<\/td>\n<td class=\"t02\">5.6<\/td>\n<td class=\"t02\">Ca<sup>2+<\/sup> indicator based on calmodulin and FRET by CFP-Venus pair. <\/td>\n<td class=\"t02\">PMID 15247428<\/td>\n<td class=\"t02\">YC2.60\/pRSETB (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15134\">RDB15134<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">YC3.60 Yellow cameleon<\/td>\n<td class=\"t02\">250<\/td>\n<td class=\"t02\">5.6<\/td>\n<td class=\"t02\">Low-affinity Ca<sup>2+<\/sup> indicator based on calmodulin and FRET by CFP-Venus pair (ie. HeLa cells and hippocampal brain slice of transgenic mouse). <\/td>\n<td class=\"t02\">PMID 15247428<\/td>\n<td class=\"t02\">YC3.60\/pcDNA3 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15135\">15135<\/a>)<br \/>\nYC3.60\/pcDNA3 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15136\">15136<\/a>)<br \/>\nYC3.60pm\/pRSETB (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15137\">15137<\/a>) <\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">YC4.60 Yellow cameleon<\/td>\n<td class=\"t02\">58<\/td>\n<td class=\"t02\">3.6<\/td>\n<td class=\"t02\">Low-affinity Ca<sup>2+<\/sup> indicator based on calmodulin and FRET by CFP-Venus pair. <\/td>\n<td class=\"t02\">PMID 15247428<\/td>\n<td class=\"t02\">YC4.60\/ pRSETB (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15138\">RDB15138<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">GeuSapVC2.60<script>newImage(2025,7,13)<\/script><\/td>\n<td class=\"t02\">71<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">Ca<sup>2+<\/sup> indicator based on FRET-induced Angular Displacement Evaluation via Dim donor<\/td>\n<td class=\"t02\">PMID 33953187<\/td>\n<td class=\"t02\">pcDNA3-GeuSapVC2.60 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB20914\">RDB20914<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">GeuSapVC3.60<script>newImage(2025,7,13)<\/script><\/td>\n<td class=\"t02\">502<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">Ca<sup>2+<\/sup> indicator based on FRET-induced Angular Displacement Evaluation via Dim donor<\/td>\n<td class=\"t02\">PMID 33953187<\/td>\n<td class=\"t02\">pcDNA3-GeuSapVC3.60 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB20915\">RDB20915<\/a>)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Pericam<\/strong><\/h3>\n<table cellspacing=\"0\" cellpadding=\"5\">\n<tbody>\n<tr valign=\"top\">\n<td class=\"t02\" style=\"text-align: center;\"><strong>probe name<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>Ca<sup>2+<\/sup>affinity (K<sub>d<\/sub>, nM)<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>dynamic range (F<sub>max<\/sub>\/F<sub>min<\/sub>)<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>features&amp;application<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>refference<\/strong><\/td>\n<td class=\"t02\" style=\"text-align: center;\"><strong>plasmid clone<br \/>\n(cat. num)<\/strong><\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">Flash-pericam<\/td>\n<td class=\"t02\">700<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">Designed to work as a single wavelength indicator of intracellular Ca<sup>2+<\/sup>. Better at 28-30<sup>o<\/sup>C than 37<sup>o<\/sup>C. (HeLa cells)<\/td>\n<td class=\"t02\">PMID 11248055<\/td>\n<td class=\"t02\">Flash pericam\/pcDNA3 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15139\">RDB15139<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">Ratiometric-pericam<\/td>\n<td class=\"t02\">1,700<\/td>\n<td class=\"t02\">10<\/td>\n<td class=\"t02\">Heat stable version. Work at 37<sup>o<\/sup>C. (HeLa cells)<\/td>\n<td class=\"t02\">PMID 11248055<\/td>\n<td class=\"t02\">Ratiometric pericam\/pcDNA3 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15143\">RDB15143<\/a>)<br \/>\nRatiometric pericam-mt\/pcDNA3 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15144\">RDB15144<\/a>)<br \/>\nRatiometric pericam-nu\/pcDNA3 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15145\">RDB15145<\/a>)<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td class=\"t02\" nowrap=\"nowrap\">Inverse pericam<\/td>\n<td class=\"t02\">200<\/td>\n<td class=\"t02\">&nbsp;<\/td>\n<td class=\"t02\">Becoming dimmed with Ca<sup>2+<\/sup>. (HeLa cells)<\/td>\n<td class=\"t02\">PMID 11248055<\/td>\n<td class=\"t02\">Inverse pericam\/pcDNA3 (<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15140\">RDB15140<\/a>)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h3>G-CaMP and R-CaMP clones<\/h3>\n<div align=\"Left\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB06747_400.png\" width=\"400\" border=\"0\" \/>\n<\/div>\n<table width=\"100%\">\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of clone<\/th>\n<th>Publication<\/th>\n<\/tr>\n<tr>\n<td><script>newImage(2022,4,12)<\/script>RDB19475<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19475\">pBS-CaMKIIpro-R-CaMP2-WPRE<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/69995\">Inoue, M., Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12 (1): 64-70 (2015). PMID 25419959.<\/a><\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2022,4,12)<\/script>RDB19474<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19474\">pBS-CaMKIIpro-XCaMP-R-WPRE<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/69996\">Inoue, M., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068.<\/a><\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2022,4,12)<\/script>RDB19473<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19473\">pBS-CaMKIIpro-XCaMP-B-WPRE<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/69996\">Inoue, M., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068.<\/a><\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2022,4,12)<\/script>RDB19472<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19472\">pBS-CaMKIIpro-XCaMP-Y-WPRE<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/69996\">Inoue, M., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068.<\/a><\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2022,4,12)<\/script>RDB19471<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19471\">pBS-CaMKIIpro-XCaMP-G-WPRE<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/69996\">Inoue, M., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068.<\/a><\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2022,4,12)<\/script>RDB19470<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19470\">pBS-CaMKIIpro-XCaMP-Gf-WPRE<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/69996\">Inoue, M., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068.<\/a><\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2022,4,12)<\/script>RDB19172<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19172\">G-CaMP9a <\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/69634\">Sakamoto, M., A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods 2: 100168 (2022).<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB14612<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14612\">G-CaMP7.09<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/47680\">Shiba, Y., Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538 (7625): 388-391 (2016). PMID 27723741.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB14611<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14611\">G-CaMP8<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/47679\">Ohkura, M., Genetically encoded green fluorescent Ca<sup>2+<\/sup> indicators with improved detectability for neuronal Ca<sup>2+<\/sup> signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB14610<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14610\">G-CaMP7<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/47679\">Ohkura, M., Genetically encoded green fluorescent Ca<sup>2+<\/sup> indicators with improved detectability for neuronal Ca<sup>2+<\/sup> signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB14609<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14609\">G-CaMP6<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/47679\">Ohkura, M., Genetically encoded green fluorescent Ca<sup>2+<\/sup> indicators with improved detectability for neuronal Ca<sup>2+<\/sup> signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB14608<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14608\">R-CaMP1.07<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/47678\">Ohkura, M., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB14607<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14607\">G-CaMP-HS<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/47677\">Muto, A., Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). PMID 21383146.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB14606<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14606\">G-CaMP4.1<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/47676\">Shindo, A., Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation. PLoS One 5 (2): e8897 (2010). PMID 20126393.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB06750<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06750\">pN1-G-CaMP2<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/28654\">Tallini, Y.N., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca<sup>2+<\/sup>indicator GCaMP2. Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB06749<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06749\">pN1-G-CaMP1.6<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/28665\">Ohkura, M., Genetically encoded bright Ca<sup>2+<\/sup> probe applicable for dynamic Ca<sup>2+<\/sup> imaging of dendritic spines. Anal. Chem., 77, 5861-5869 (2005). PMID 16159115.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB06748<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06748\">pN1-G-CaMP1.3<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/29151\">Nakai, J., A high signal-to-noise Ca<sup>2+<\/sup> probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB06747<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06747\">pN1-G-CaMP<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/29151\">Nakai, J., A high signal-to-noise Ca<sup>2+<\/sup> probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Related Articles<\/h4>\n<ul>\n<li>Arizono, M., Bannai, H., Nakamura, K., Niwa, F., Enomoto, M., Matsu-ura, T., Miyamoto, A., Sherwood, M.W., Nakamura, T., Mikoshiba, K. Receptor-Selective Diffusion Barrier Enhances Sensitivity of Astrocytic Processes to Metabotropic Glutamate Receptor Stimulation. Science Signaling, 2012. doi: 10.1126\/scisignal.2002498<\/li>\n<li><a href=\"http:\/\/www.riken.jp\/r-world\/info\/release\/press\/2012\/120330\/index.html\">Press Release (2012, in Japanese)<\/a><\/li>\n<li><a href=\"http:\/\/www.riken.jp\/r-world\/research\/results\/2008\/080508\/index.html\">Press Release (2008, in Japanese)<\/a><\/li>\n<\/ul>\n<hr \/>\n<h3>Yellow Cameleon clones (Dr. Atsushi Miyawaki, RIKEN BSI)<\/h3>\n<table>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of clone<\/th>\n<th>Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB15132<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15132\">YC2.12\/pCS2<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49219\">Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15134<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15134\">YC2.60\/pRSETB<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49218\">Nagai, T., Expanded dynamic range of fluorescent indicators for Ca<sup>2+<\/sup> by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15455<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15455\">YCX2.60\/pCS2<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49627\">Madisen, L., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15133<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15133\">YC3.12\/pCS2<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49218\">Nagai, T., Expanded dynamic range of fluorescent indicators for Ca<sup>2+<\/sup> by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15135<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15135\">YC3.60\/pcDNA3<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49218\">Nagai, T., Expanded dynamic range of fluorescent indicators for Ca<sup>2+<\/sup> by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15136<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15136\">YC3.60\/pRSETB<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49218\">Nagai, T., Expanded dynamic range of fluorescent indicators for Ca<sup>2+<\/sup> by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15137<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15137\">YC3.60pm\/pRSETB<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49218\">Nagai, T., Expanded dynamic range of fluorescent indicators for Ca<sup>2+<\/sup> by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15138<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15138\">YC4.60\/pRSETB<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49218\">Nagai, T., Expanded dynamic range of fluorescent indicators for Ca<sup>2+<\/sup> by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.<\/a><\/td>\n<\/tr>\n<\/table>\n<h4>Related Articles<\/h4>\n<ul>\n<li><a href=\"http:\/\/www.tmd.ac.jp\/mri\/press\/press34\/index.html\">Press Release (2016, in Japanese)<\/a><\/li>\n<li><a href=\"http:\/\/www.tmd.ac.jp\/mri\/press\/press32\/index.html\">Press Release (2016, in Japanese)<\/a><\/li>\n<li><a href=\"http:\/\/www.naist.jp\/pressrelease\/2015\/09\/000632.html\">Press Release (2015, in Japanese)<\/a><\/li>\n<\/ul>\n<hr \/>\n<h3>Pericam clones (Dr. Atsushi Miyawaki, RIKEN BSI)<\/h3>\n<table>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of clone<\/th>\n<th>Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB15139<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15139\">Flash pericam\/pcDNA3<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49237\">Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15140<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15140\">Inverse pericam\/pcDNA3<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49237\">Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15141<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15141\">Split pericam-N\/pcDNA3<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49237\">Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15142<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15142\">Split pericam-C\/pcDNA3<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49237\">Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15143<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15143\">Ratiometric pericam\/pcDNA3<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49237\">Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15144<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15144\">Ratiometric pericam-mt\/pcDNA3<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49237\">Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15145<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15145\">Ratiometric pericam-nu\/pcDNA3<\/a><\/td>\n<td><a href=\"https:\/\/rrc.nbrp.jp\/references\/49237\">Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.<\/a><\/td>\n<\/tr>\n<\/table>\n<h4>Related Articles<\/h4>\n<ul>\n<li><\/li>\n<\/ul>\n<hr \/>\n<p style=\"text-align:left;\">2025.07.13 (T.M. GRP0029e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Calcium-ion sensor clones Fluorescent protein-based Ca2+ sensors, which are composed with calmodulin, fluorescent protein and M13 peptide (CaM binding domain of myosin light chain kinase), are designated to visualize intracellular [Ca2+] dynamics. Various improvements of the Ca2+ sensors have been made for optimum temperature, signal\/noise ratio, susceptibility and so on. Many papers using Ca2+ sensors have been published reporting the estimation of calcium concentration. G-CaMP and R-CaMP probe name Ca2+affinity (Kd, nM) dynamic range (Fmax\/Fmin) features&amp;application refference plasmid clone (cat. num) G-CaMP1.6 146 4.9 Brightness improved version. Work at 25oC (hippocampal slice of rat) to 28oC (HEK293 cell, HeLa cell) but not at 37oC (HEK293 cell). PMID 11175727, 16159115, 16537386 pN1-G-Camp1.6 (RDB06749) G-CaMP2 146 3.9 to 4.9 Heat stable version. Work at 37oC (HEK293 cell, heart of mouse embryo, primary culture of hippocampal astrocytes). PMID 16537386, 22472649 pN1-G-CaMP2 (RDB06750) G-CaMP-HS 102 4.1 High-Ca2+ affinity version (ie: mammalian cell, zebrafish). PMID 21383146, 27239939 G-CaMP-HS (RDB14607) G-CaMP6 158 11.4 to 27.9 Sensitivity improved version. Quick ON\/OFF response allow to observe neuronal-cell calcium spikes (ie: mouse brain section, mouse B cell, C. elegans). PMID 23240011, 27385517, 27428827 G-CaMP6 (RDB14609) pMXs-IRES-hNGFR_G-CaMP6 (RDB14513) G-CaMP7.09 212 19.3 Enhanced brightness allow to observe flashing fluorescence of iPS [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8590,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8605","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8605","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8605"}],"version-history":[{"count":3,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8605\/revisions"}],"predecessor-version":[{"id":9028,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8605\/revisions\/9028"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8590"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8605"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}