{"id":8600,"date":"2025-05-03T15:58:13","date_gmt":"2025-05-03T06:58:13","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8600"},"modified":"2025-05-03T15:58:48","modified_gmt":"2025-05-03T06:58:48","slug":"atpimaging01en","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/biosensor01en\/atpimaging01en","title":{"rendered":"ATP imaging"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h4>ATP imaging<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform.<br \/>\nZhong, C., Arai, S., Okada, Y.<br \/>\nCell Rep. Methods 4 (11): 100902 (2024). PubMed PMID 39561716.<\/p>\n<table>\n<tr>\n<th>Catalog number<\/Th><\/p>\n<th>Name of Resource<\/Th><\/p>\n<th>Description<\/Th><br \/>\n   <\/tr>\n<tr>\n<td>RDB20448<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB20448\">pcDNA3.1(-)-qmTQ2-ATP<\/a><\/td>\n<td>Mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging.<\/td>\n<\/tr>\n<tr>\n<td>RDB20447<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB20447\">pRSETA-qmTQ2-ATP<\/a><\/td>\n<td>Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging.<\/td>\n<\/tr>\n<\/table>\n<\/li>\n<\/ul>\n<hr width=\"50%\" \/>\n<ul style=\"line-height: 1.5em;\">\n<li>Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging.<br \/>\nYaginuma, H., Kawai, S., Tabata, K.V., Tomiyama, K., Kakizuka, A., Komatsuzaki, T., Noji, H., Imamura, H.<br \/>\nSci. Rep. 4: 6522 (2014). PubMed PMID 25283467.<\/p>\n<table>\n<tr>\n<th>Catalog number<\/Th><\/p>\n<th>Name of Resource<\/Th><\/p>\n<th>Description<\/Th><br \/>\n   <\/tr>\n<tr>\n<td>RDB17766<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17766\">pN1-QUEEN7mu<\/a><\/td>\n<td>Low concentration ATP measurement<\/td>\n<\/tr>\n<tr>\n<td>RDB17783<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17783\">pN1-ITPKA-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, actin<\/td>\n<\/tr>\n<tr>\n<td>RDB17777<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17777\">pN1-COX-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, mitochondria matrix<\/td>\n<\/tr>\n<tr>\n<td>RDB17784<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17784\">pN1-TOMM20-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, mitochondria outer membrane<\/td>\n<\/tr>\n<tr>\n<td>RDB17771<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17771\">pN1-NLS-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, nucleus<\/td>\n<\/tr>\n<tr>\n<td>RDB17758<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17758\">pN1-PTS2-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, peroxisome<\/td>\n<\/tr>\n<tr>\n<td>RDB17773<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17773\">pN1-SSTRv2-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, primary cilia<\/td>\n<\/tr>\n<tr>\n<td>RDB17760<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17760\">pN1-Tubbeta5-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, Tubulin<\/td>\n<\/tr>\n<tr>\n<td>RDB17762<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17762\">pT2P-UB-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, Tol2 transposon<\/td>\n<\/tr>\n<tr>\n<td>RDB17761<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17761\">pT2P-EF-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, Tol2 transposon<\/td>\n<\/tr>\n<tr>\n<td>RDB17780<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17780\">pT2MP.EF-ITPKA-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, actin, Tol2 transposon<\/td>\n<\/tr>\n<tr>\n<td>RDB17772<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17772\">pT2MP.EF-TOMM-Q7mu<\/a><\/td>\n<td>Low concentration ATP measurement, mitochondria outer membrane, Tol2 transposon<\/td>\n<\/tr>\n<tr>\n<td>RDB17770<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17770\">pRS-QUE7mu<\/a><\/td>\n<td>Low concentration ATP measurement<\/td>\n<\/tr>\n<tr>\n<td>RDB17767<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17767\">pN1-QUE2m<\/a><\/td>\n<td>QUEEN ATP sensor for 25degC<\/td>\n<\/tr>\n<tr>\n<td>RDB17781<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17781\">pN1-ITPKA-Q2m<\/a><\/td>\n<td>QUEEN ATP sensor for 25degC, actin<\/td>\n<\/tr>\n<tr>\n<td>RDB17779<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17779\">pN1-COX-Q2m<\/a><\/td>\n<td>QUEEN ATP sensor for 25degC, mitochondria matrix<\/td>\n<\/tr>\n<tr>\n<td>RDB17775<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17775\">pN1-NLS-Q2m<\/a><\/td>\n<td>QUEEN ATP sensor for 25degC, nucleus<\/td>\n<\/tr>\n<tr>\n<td>RDB17769<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17769\">pT2MP.EF-Q2m<\/a><\/td>\n<td>QUEEN ATP sensor for 25degC, Tol2 transposon<\/td>\n<\/tr>\n<tr>\n<td>RDB17763<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17763\">pT2MP.UB-Q2m<\/a><\/td>\n<td>QUEEN ATP sensor for 25degC, Tol2 transposon<\/td>\n<\/tr>\n<tr>\n<td>RDB17785<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17785\">pT2MP.EF-NLS-Q2m<\/a><\/td>\n<td>QUEEN ATP sensor for 25degC, nucleus, Tol2 transposon<\/td>\n<\/tr>\n<tr>\n<td>RDB17768<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17768\">pN1-QNA<\/a><\/td>\n<td>Negative control for ATP measurement<\/td>\n<\/tr>\n<tr>\n<td>RDB17755<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17755\">pN1-QUEEN7mu R122K R126K<\/a><\/td>\n<td>Negative control for ATP measurement<\/td>\n<\/tr>\n<tr>\n<td>RDB17782<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17782\">pN1-ITPKA-QNA<\/a><\/td>\n<td>Negative control for ATP measurement, actin<\/td>\n<\/tr>\n<tr>\n<td>RDB17778<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17778\">pN1-COX-QNA<\/a><\/td>\n<td>Negative control for ATP measurement, mitochondria matrix<\/td>\n<\/tr>\n<tr>\n<td>RDB17757<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17757\">pN1-TOMM20-QNA<\/a><\/td>\n<td>Negative control for ATP measurement, mitochondria outer membrane<\/td>\n<\/tr>\n<tr>\n<td>RDB17774<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17774\">pN1-NLS-QNA<\/a><\/td>\n<td>Negative control for ATP measurement, Nucleus<\/td>\n<\/tr>\n<tr>\n<td>RDB17756<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17756\">pN1-PTS2-QNA<\/a><\/td>\n<td>Negative control for ATP measurement, Peroxisome<\/td>\n<\/tr>\n<tr>\n<td>RDB17759<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17759\">pN1-Tubbeta5-QNA<\/a><\/td>\n<td>Negative control for ATP measurement, Tubulin<\/td>\n<\/tr>\n<tr>\n<td>RDB17764<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17764\">pRSET-QNA<\/a><\/td>\n<td>Negative control for ATP measurement, G3C mutation fixed (2019.11.19)<\/td>\n<\/tr>\n<tr>\n<td>RDB17765<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17765\">pT2MP.EF-QNA<\/a><\/td>\n<td>Negative control for ATP measurement, Tol2 transposon<\/td>\n<\/tr>\n<tr>\n<td>RDB17776<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17776\">pT2MP.EF-TOMM-QNA<\/a><\/td>\n<td>Negative control for ATP measurement, mitochondria outer membrane, Tol2 transposon<\/td>\n<\/tr>\n<\/table>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p style=\"text-align:left;\">2025.05.03 (T.M. GRP0066e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ATP imaging Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform. Zhong, C., Arai, S., Okada, Y. Cell Rep. Methods 4 (11): 100902 (2024). PubMed PMID 39561716. Catalog number Name of Resource Description RDB20448 pcDNA3.1(-)-qmTQ2-ATP Mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging. RDB20447 pRSETA-qmTQ2-ATP Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging. Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging. Yaginuma, H., Kawai, S., Tabata, K.V., Tomiyama, K., Kakizuka, A., Komatsuzaki, T., Noji, H., Imamura, H. Sci. Rep. 4: 6522 (2014). PubMed PMID 25283467. Catalog number Name of Resource Description RDB17766 pN1-QUEEN7mu Low concentration ATP measurement RDB17783 pN1-ITPKA-Q7mu Low concentration ATP measurement, actin RDB17777 pN1-COX-Q7mu Low concentration ATP measurement, mitochondria matrix RDB17784 pN1-TOMM20-Q7mu Low concentration ATP measurement, mitochondria outer membrane RDB17771 pN1-NLS-Q7mu Low concentration ATP measurement, nucleus RDB17758 pN1-PTS2-Q7mu Low concentration ATP measurement, peroxisome RDB17773 pN1-SSTRv2-Q7mu Low concentration ATP measurement, primary cilia RDB17760 pN1-Tubbeta5-Q7mu Low concentration ATP measurement, Tubulin RDB17762 pT2P-UB-Q7mu Low concentration ATP measurement, Tol2 transposon RDB17761 pT2P-EF-Q7mu Low concentration ATP measurement, Tol2 transposon RDB17780 pT2MP.EF-ITPKA-Q7mu Low concentration ATP measurement, actin, Tol2 transposon RDB17772 pT2MP.EF-TOMM-Q7mu [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8590,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8600","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8600","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8600"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8600\/revisions"}],"predecessor-version":[{"id":8604,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8600\/revisions\/8604"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8590"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8600"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}