{"id":8595,"date":"2025-05-03T15:56:48","date_gmt":"2025-05-03T06:56:48","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8595"},"modified":"2025-05-03T15:57:32","modified_gmt":"2025-05-03T06:57:32","slug":"fucci01en","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/biosensor01en\/fucci01en","title":{"rendered":"Fucci cell cycle indicator"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>Fucci &#8211; Fluorescent Ubiquitination-based Cell Cycle Indicator<\/h2>\n<p><a name=\"dep102388\"><\/a><\/p>\n<h3><span style=\"color: #0000ff;\">Fucci5<\/span><\/h3>\n<p style=\"text-decoration: underline;\">Ando, R., et al. Cell Struct. Funct. 48 (2): 135-144 (2023). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37394513\">PMID 37394513<\/a>.<\/p>\n<h4>DNA materials<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB18220<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18220\">tFucci(SA)5\/pCS2<\/a><\/td>\n<td>CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3<\/td>\n<\/tr>\n<tr>\n<td>RDB18221<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18221\">tFucci(CA)5\/pCS2<\/a><\/td>\n<td>CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Cell line<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<\/tr>\n<tr>\n<td>RCB4917<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB4917\">HeLa\/Fucci(SA)5#16<\/a><\/td>\n<\/tr>\n<tr>\n<td>RCB4919<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB4919\">HeLa\/Fucci(CA)5#16<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><span style=\"color: #0000ff;\">Cell cycle indicator Fucci(CA) and Fucci(SA)<\/span><\/h3>\n<p style=\"text-decoration: underline;\">Sakaue-Sawano, A. et al., Mol. Cell 68 (3): 626-640.e5 (2017). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29107535\">PMID 29107535<\/a>.<\/p>\n<h4>DNA materials<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB15459<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15459\">mCherry-hCdt1(1\/100)Cy(-)\/pcDNA3<\/a><\/td>\n<td>CMV promoter, hCdt1, mCherry<\/td>\n<\/tr>\n<tr>\n<td>RDB15273<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15273\">mCherry-hCdt1(30\/120) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15441<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15441\">mCherry-hCdt1(1\/100)Cy(-)\/pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15442<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15442\">mCherry-hCdt1(1\/100)\/pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15271<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15271\">mVenus-hGeminin(1\/110) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hGeminin, mVenus, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15446<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15446\">tFucci(CA)2\/pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15452<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15452\">tFucci(CA)2\/pCSII-CMV<\/a><\/td>\n<td>CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15447<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15447\">tFucci(CA)2.1\/pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15453<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15453\">tFucci(CA)2.1\/pCSII-CMV<\/a><\/td>\n<td>CMV promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15448<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15448\">tFucci(CA)2.2\/pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15454<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15454\">tFucci(CA)2.2\/pCSII-CMV<\/a><\/td>\n<td>CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15443<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15443\">tFucci(SA)2\/pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15449<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15449\">tFucci(SA)2\/pCSII-CMV<\/a><\/td>\n<td>CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15444<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15444\">tFucci(SA)2.1\/pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15450<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15450\">tFucci(SA)2.1\/pCSII-CMV<\/a><\/td>\n<td>CMV promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15445<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15445\">tFucci(SA)2.2\/pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15451<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15451\">tFucci(SA)2.2\/pCSII-CMV<\/a><\/td>\n<td>CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Cell line<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<\/tr>\n<tr>\n<td>RCB4676<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB4676\">HeLa\/Fucci(CA)2<\/a><\/td>\n<\/tr>\n<tr>\n<td>RCB4675<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB4675\">NMuMG\/Fucci(CA)2.1<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a name=\"fucci2a\"><\/a><\/p>\n<h3><span style=\"color: #0000ff;\">Cell cycle indicator Fucci2a<\/span><\/h3>\n<p style=\"text-decoration: underline;\">Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30458140\">PMID 30458140<\/a>.<br \/>\nMort, R.L. et al., Cell Cycle 13 (17): 2681-2696 (2014). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25486356\">PMID 25486356<\/a>.<\/p>\n<h4>DNA materials<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB16057<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16057\">pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a<\/a><\/td>\n<td>CAG promoter, Arl13b, Cerulean, hGem(1\/110), mVenus, hCdt1(30\/120), mCherry<\/td>\n<\/tr>\n<tr>\n<td>RDB16058<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16058\">pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a<\/a><\/td>\n<td>>CAG promoter, Arl13b, Cerulean, hGem(1\/110), mVenus, hCdt1(30\/120), mCherry<\/td>\n<\/tr>\n<tr>\n<td>RDB16059<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16059\">pCAG-Arl13bCerulean-Fucci2a<\/a><\/td>\n<td>>CAG promoter, Arl13b, Cerulean, hGem(1\/110), mVenus, hCdt1(30\/120), mCherry<\/td>\n<\/tr>\n<tr>\n<td>RDB13080<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13080\">pCAG-Fucci2a<\/a><\/td>\n<td>CAG promoter, hGem(1\/110), mVenus, hCdt1(30\/120), mCherry<\/td>\n<\/tr>\n<tr>\n<td>RDB13081<\/td>\n<td>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB13081\">pROSA-floxNeo-Fucci2a<\/a><\/td>\n<td>CAG promoter, hGem(1\/110), mVenus, hCdt1(30\/120), mCherry<\/td>\n<\/tr>\n<tr>\n<td>RDB16056<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16056\">pCDNA5-Frt-Fucci2a<\/a><\/td>\n<td>CMV promoter, hGem(1\/110), mVenus, hCdt1(30\/120), mCherry<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Mouse<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<\/tr>\n<tr>\n<td>RBRC 06511<\/td>\n<td><a href=\"https:\/\/knowledge.brc.riken.jp\/resource\/animal\/card?brc_no=RBRC06511\">B6;129-Gt(ROSA)26Sor&lt;tm1(Fucci2aR)Jkn&gt;<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Cell line<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<\/tr>\n<tr>\n<td>AES0223<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=AES0223\">MESC Fucci2aR<\/a><\/td>\n<\/tr>\n<tr>\n<td>AES0224<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=AES0224\">MESC Fucci2a<\/a><\/td>\n<\/tr>\n<tr>\n<td>RCB5029<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB5029\">NIH3T3 Flp Arl13bCerulean-Fucci2a<\/a><\/td>\n<\/tr>\n<tr>\n<td>RCB5030<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB5030\">NIH3T3 Flp Fucci2a<\/a><\/td>\n<\/tr>\n<tr>\n<td>AES0225<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=AES0225\">MESC Arl13b-Fucci2aR<\/a><\/td>\n<\/tr>\n<tr>\n<td>AES0226<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=AES0226\">MESC Arl13b-Fucci2a<\/a>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><span style=\"color: #0000ff;\">Cell cycle indicator Fucci2<\/span><\/h3>\n<p style=\"text-decoration: underline;\">Sakaue-Sawano, A. et al., BMC Cell Biol. 12: 2 (2011). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/21226962\">PMID 21226962<\/a>.<br \/>\nSakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/9101468\">PMID 9101468<\/a>.<\/p>\n<h4>DNA materials<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB15457<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15457\">mCherry-hCdt1(30\/120)\/pcDNA3<\/a><\/td>\n<td>CMV promoter, hCdt1, mCherry<\/td>\n<\/tr>\n<tr>\n<td>RDB15458<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15458\">mVenus-hGeminin(1\/110)\/pcDNA3<\/a><\/td>\n<td>CMV promoter, hGeminin, mVenus<\/td>\n<\/tr>\n<tr>\n<td>RDB15273<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15273\">mCherry-hCdt1(30\/120) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mCherry, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15271<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15271\">mVenus-hGeminin(1\/110) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hGeminin, mVenus, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15274<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15274\">AmCyan-hGem(1\/110) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hGeminin, AmCyan, lentivirus vector<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Cell line<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<\/tr>\n<tr>\n<td>RCB2867<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB2867\">HeLa\/Fucci2<\/a><\/td>\n<\/tr>\n<tr>\n<td>RCB2868<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB2868\">NMuMG\/Fucci2<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><span style=\"color: #0000ff;\">Cell cycle indicator Fucci<\/span><\/h3>\n<p style=\"text-decoration: underline;\">Sakaue-Sawano, A. et al., Development 140 (22): 4624-4632 (2013). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/24154524\">PMID 24154524<\/a>.<br \/>\nAiba, Y, et al., Proc. Natl. Acad. Sci. USA. 107 (27): 12192-12197 (2010). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/20547847\">PMID 20547847<\/a>.<br \/>\nSakaue-Sawano, A., Cell 132 (3): 487-498 (2008). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/18267078\">PMID 18267078<\/a>.<\/p>\n<h4>DNA materials<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB15267<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15267\">mKO2-hCdt1(30\/120) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hCtd1, mKO2, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15268<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15268\">mAG-hGeminin(1\/110) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hGeminin, mAG, lentivirus vector<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Mouse<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<\/tr>\n<tr>\n<td>RBRC02706<\/td>\n<td><a href=\"https:\/\/knowledge.brc.riken.jp\/resource\/animal\/card?brc_no=RBRC02706\">B6.Cg-Tg (FucciS\/G2\/M) #504Bsi<\/a><\/td>\n<\/tr>\n<tr>\n<td>RBRC02707<\/td>\n<td><a href=\"https:\/\/knowledge.brc.riken.jp\/resource\/animal\/card?brc_no=RBRC02707\">B6.Cg-Tg (FucciG1) #596Bsi<\/a><\/td>\n<\/tr>\n<tr>\n<td>RBRC02704<\/td>\n<td><a href=\"https:\/\/knowledge.brc.riken.jp\/resource\/animal\/card?brc_no=RBRC02704\">B6.Cg-Tg(FucciS\/G2\/M)#474Bsi<\/a><\/td>\n<\/tr>\n<tr>\n<td>RBRC02708<\/td>\n<td><a href=\"https:\/\/knowledge.brc.riken.jp\/resource\/animal\/card?brc_no=RBRC02708\">B6.Cg-Tg(FucciG1)#610Bsi<\/a><\/td>\n<\/tr>\n<tr>\n<td>RBRC02705<\/td>\n<td><a href=\"https:\/\/knowledge.brc.riken.jp\/resource\/animal\/card?brc_no=RBRC02705\">B6.Cg-Tg(FucciS\/G2\/M)#492Bsi<\/a><\/td>\n<\/tr>\n<tr>\n<td>RBRC02709<\/td>\n<td><a href=\"https:\/\/knowledge.brc.riken.jp\/resource\/animal\/card?brc_no=RBRC02709\">B6.Cg-Tg(FucciG1)#639Bsi<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Cell line<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<\/tr>\n<tr>\n<td>RCB2812<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB2812\">HeLa\/Fucci<\/a><\/td>\n<\/tr>\n<tr>\n<td>RCB2813<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB2813\">NMuMG\/Fucci<\/a><\/td>\n<\/tr>\n<tr>\n<td>RCB2814<\/td>\n<td><a href=\"https:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB2814\">COS\/Fucci<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><span style=\"color: #0000ff;\">Cell cycle indicator<\/span><\/h3>\n<p style=\"text-decoration: underline;\">Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19101468\">PMID 19101468<\/a>.<\/p>\n<h4>DNA materials<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB15269<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15269\">mCherry-hGeminin(1\/110) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hGeminin, mCherry, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15270<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15270\">mCherry-hGeminin(1\/60) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hGeminin, mCherry, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15272<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15272\">mVenus-hGeminin(1\/60) \/ pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hGeminin, mVenus, lentivirus vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15274<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15274\">AmCyan-hGem(1\/110)\/pCSII-EF<\/a><\/td>\n<td>EF-1\u03b1 promoter, hGeminin, AmCyan, lentivirus vector<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><span style=\"color: #0000ff;\">Tol2 plasmid<\/span><\/h3>\n<p style=\"text-decoration: underline;\">Sugiyama, M. et al., Proc. Natl. Acad. Sci. USA. 106 (49): 20812-20817 (2009). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19923430\">PMID 19923430<\/a>.<\/p>\n<h4>DNA materials<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Cat. no.<\/th>\n<th>Resource name<\/th>\n<\/tr>\n<tr>\n<td>RDB15278<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15278\">mCherry-zCdt1(1\/190) \/ pT2KXIGdeltain<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15279<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15279\">mVenus-zGeminin (1\/100) \/ pT2KXIGdeltain<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15275<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15275\">mKO2-zCdt1(1\/190) \/ pT2KXIGdeltain<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15276<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15276\">mAG-zGeminin(1\/100) \/ pT2KXIGdeltain<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB15277<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15277\">mAG-hGeminin (1\/60) \/ pT2KXIGdeltain<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><span style=\"color: #0000ff;\">Other Fucci related materials<\/h3>\n<p><a href=\"https:\/\/cfds.riken.jp\/material\/fucci\">RIKEN Center for Brain Science (CBS), Dr. Miyawaki lab.<\/a><\/p>\n<hr \/>\n<h2>References<\/h2>\n<h3>Important notice regarding nomenclature of Fucci<\/h3>\n<ul>\n<li>Please read this <a href=\"https:\/\/cfds.riken.jp\/material\/fucci\">statement by Dr. Miyawaki [link]<\/a><\/li>\n<\/ul>\n<h3>Mouse of the Month<\/h3>\n<ul>\n<li><a href=\"https:\/\/mus.brc.riken.jp\/en\/mouse_of_month\/mar_2009_mm\">March 2009: Fucci mice for visualizing cell-cycle in vivo [link]<\/a><\/li>\n<\/ul>\n<p style=\"text-align:left;\">2025.05.03 (T.M. GRP0044e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fucci &#8211; Fluorescent Ubiquitination-based Cell Cycle Indicator Fucci5 Ando, R., et al. Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513. DNA materials Cat. no. Resource name Description RDB18220 tFucci(SA)5\/pCS2 CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3 RDB18221 tFucci(CA)5\/pCS2 CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3 Cell line Cat. no. Resource name RCB4917 HeLa\/Fucci(SA)5#16 RCB4919 HeLa\/Fucci(CA)5#16 Cell cycle indicator Fucci(CA) and Fucci(SA) Sakaue-Sawano, A. et al., Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. DNA materials Cat. no. Resource name Description RDB15459 mCherry-hCdt1(1\/100)Cy(-)\/pcDNA3 CMV promoter, hCdt1, mCherry RDB15273 mCherry-hCdt1(30\/120) \/ pCSII-EF EF-1\u03b1 promoter, hCtd1, mCherry, lentivirus vector RDB15441 mCherry-hCdt1(1\/100)Cy(-)\/pCSII-EF EF-1\u03b1 promoter, hCtd1, mCherry, lentivirus vector RDB15442 mCherry-hCdt1(1\/100)\/pCSII-EF EF-1\u03b1 promoter, hCtd1, mCherry, lentivirus vector RDB15271 mVenus-hGeminin(1\/110) \/ pCSII-EF EF-1\u03b1 promoter, hGeminin, mVenus, lentivirus vector RDB15446 tFucci(CA)2\/pCSII-EF EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector RDB15452 tFucci(CA)2\/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector RDB15447 tFucci(CA)2.1\/pCSII-EF EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector RDB15453 tFucci(CA)2.1\/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector RDB15448 tFucci(CA)2.2\/pCSII-EF EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector RDB15454 tFucci(CA)2.2\/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector RDB15443 tFucci(SA)2\/pCSII-EF EF-1\u03b1 promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector RDB15449 tFucci(SA)2\/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8590,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8595","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8595","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8595"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8595\/revisions"}],"predecessor-version":[{"id":8599,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8595\/revisions\/8599"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8590"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8595"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}