{"id":8565,"date":"2025-05-03T11:24:12","date_gmt":"2025-05-03T02:24:12","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8565"},"modified":"2025-06-05T17:47:48","modified_gmt":"2025-06-05T08:47:48","slug":"luciferaseen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/luminescenceen\/luciferaseen","title":{"rendered":"Luciferase"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>Luciferase<\/h2>\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"#akaluc\">Akaluc luciferase providing brighter and red-shifted luminescence<\/a><\/li>\n<li><a href=\"#l\">Brighter than commonly used luciferase!<\/a><\/li>\n<li><a href=\"#aluc\">Artificial Luciferase Variants<\/a><\/li>\n<li><a href=\"#bacteria\">Bacterial luciferase<\/a><\/li>\n<li><a href=\"#JNC\">JNC Corporation&#8217;s Resource<\/a><\/li>\n<li><a href=\"#control\">Positeve control vector<\/a><\/li>\n<\/ul>\n<p><a name=\"akaluc\"><\/a><\/p>\n<h3>Akaluc luciferase providing brighter and red-shifted luminescence<\/h3>\n<p>Firefly bioluminescence systems have been commonly used as a imaging tool of biological phenomena. However, it have not been strong enough for imaging signals in deep tissues, because of low permeabilization of substrates. Recently, Dr. Atsushi Miyawaki and Dr. Satoshi Iwano of the RIKEN Center for Brain Science, and Dr. Shojiro Maki of the University of Electro-Communications developed a system of artificial bioluminescence AkaBLI that enables noninvasive signal observation in deep tissue of living animals. <\/p>\n<p>The AkaBLI system consists of an artificial substrate AkaLumine with improved tissue permeability and an artificial luciferase Akaluc optimized to AkaLumine. The intensity of the luminescence of AkaBLI system is 100 to 1000 folds brighter than the conventional systems.<br \/>\nThe expression vector of artificial luciferase Akaluc has been deposited by Dr. Miyawaki&#8217;s lab and it is now available from the DNA Bank.<\/p>\n<ul>\n<li>Reference<br \/>\nNakashiba, T. et al., Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab. Anim. (NY). 2023 Sep 7. doi: 10.1038\/s41684-023-01238-6. Epub ahead of print. PMID 37679611.<br \/>\nIwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.<\/li>\n<li>Press Release and Blog<br \/>\n<a href=\"https:\/\/web.brc.riken.jp\/en\/archives\/news\/20230908_01\">Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging.<\/a> (2023\/9\/8, RIKEN BRC)<script>newImage(2023,09,13)<\/script><br \/>\n<a href=\"http:\/\/www.riken.jp\/en\/pr\/press\/2018\/20180223_1\/\">In living color: seeing cells from outside the body with synthetic bioluminescence<\/a> (2018\/2\/23, RIKEN)<br \/>\n<a href=\"http:\/\/itaintmagic.riken.jp\/hot-off-the-press\/synthetic-bioluminescence\/\">In living color: imaging the brain with synthetic bioluminescence<\/a> (2018\/2\/23, It Ain&#8217;t Magic Blog)<\/li>\n<li>DNA resource<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15781\">pcDNA3 Venus-Akaluc (cat# RDB15781)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15782\">pAAV2 SynTetOff Venus-Akaluc (cat# RDB15782)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15783\">pAAV2 TRE Venus-Akaluc (cat# RDB15783)<\/a><\/li>\n<\/ul>\n<p style=\"text-align: right;\">(2018.05.07 N.N.)<\/p>\n<hr \/>\n<p><a name=\"l\"><\/a><\/p>\n<h3>Brighter than commonly used luciferase!<\/h3>\n<p>These luciferases have at most four-time maximum luminous intensity than that of widely used luciferase of the <i>Photinus pyralis<\/i>, a common North American firefly. These luciferases as a reporter, allow to monitor gene expression with high sensitivity and time-lapse observation.<\/p>\n<table>\n<tbody>\n<tr valign=\"top\">\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_001.png\" width=\"300\">\n<\/td>\n<td valign=\"top\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_002.png\" width=\"300\">\n<\/td>\n<\/tr>\n<tr>\n<td  colspan=\"2\" valign=\"top\">\nObservation of luminescence of transfected HeLa cells.<br \/>\nHeLa cells were transfected with expression vectors harbouring luciferase coding regions, respectively. Luminescence was observed under the condition of 2 mM D-luciferin. Left, PmatLuc1, DkumLuc1, MALuci2Luc. Right, SflaRE1Luc, PsagRE1Luc.\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table width=\"100%\" border=\"1\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#ffffff\">\n<tbody>\n<tr>\n<th rowspan=\"2\">Catalog no.<\/th>\n<th rowspan=\"2\">Name of clone<\/th>\n<th rowspan=\"2\">Origin<\/th>\n<th rowspan=\"2\">Intracellular luminescence intensity(vie, Phothinus pyralis)<\/th>\n<th colspan=\"2\">Maximal absorption wavelength (pH8, in vitro)<\/th>\n<th rowspan=\"2\">misc.<\/th>\n<\/tr>\n<tr>\n<th>25\u2103<\/th>\n<th>37\u2103<\/th>\n<tr>\n<td>RDB14359<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14359\">pPmatLuc1<\/a><\/td>\n<td>Pyrocoelia matsumurai<\/a><\/td>\n<td>4 times or more<\/td>\n<td>560 nm<\/td>\n<td>567 nm<\/td>\n<td rowspan=\"5\">luciferase cDNA is cloned in pUC19 vector.<\/td>\n<\/tr>\n<tr>\n<td>RDB14360<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14360\">pDkumLuc1<\/a><\/td>\n<td>Drilaster kumejimensis<\/a><\/td>\n<td>4 times<\/td>\n<td>558 nm<\/td>\n<td>562 nm<\/td>\n<\/tr>\n<tr>\n<td>RDB14363<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14363\">pMALuci2Luc<\/a><\/td>\n<td>Malaysian Luciola sp. (1)<\/a><\/td>\n<td>2 times<\/td>\n<td>556 nm<\/td>\n<td>557 nm<\/td>\n<\/tr>\n<tr>\n<td>RDB14362<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14362\">pSflaRE1Luc<\/a><\/td>\n<td>Stenocladius flavipennis<\/a><\/td>\n<td>2 times<\/td>\n<td>608 nm<\/td>\n<td>609 nm<\/td>\n<\/tr>\n<tr>\n<td>RDB14361<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14361\">pPsagRE1Luc<\/a><\/td>\n<td>Pristolycus sagulatus<\/a><\/td>\n<td>2 times<\/td>\n<td>605 nm<\/td>\n<td>606 nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><sup>1<\/sup>The luciferase gene originated from Malaysian firefly was developed together with Perak State Development Corporation and Nimura Genetic Solutions in conformity with the Convention on Biological Diversity.\n<\/p>\n<h3>Multiple colors with one substrate!<\/h3>\n<p>Green-, Yellow- or Red-emission can be obtained by using D-luciferin as a substrate of each luciferase. Simultaneous monitoring of two genes can be allowed by using green- and red-luciferases.<\/p>\n<h3>High stabilities under various pH conditions!<\/h3>\n<p>These luciferases are less susceptible under variety of pH or temperature and the stable results can be obtained. Please visit our web site to see results of luminescence at 25&#8451; and 37&#8451;.\n<\/p>\n<table>\n<tbody>\n<tr>\n<td colspan=\"2\">Purified recombinant luciferases were mixed with GTA Buffer (final conc. 200 mM), ATP (final conc. 2 mM), MgSO<sub>4<\/sub> (final conc. 2 mM), D-Luciferin (final conc. 1 mM) and luminescence under the different pH conditions were observed. Left, 25&#8451;. Right, 37&#8451;.\n<\/td>\n<\/tr>\n<tr>\n<td>PmatLuc1<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_003.png\" width=\"500\"><\/td>\n<\/tr>\n<tr>\n<td>DkumLuc1<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_004.png\" width=\"500\"><\/td>\n<\/tr>\n<tr>\n<td>MALuci2Luc<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_007.png\" width=\"500\"><\/td>\n<\/tr>\n<tr>\n<td>PsagRE1Luc<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_005.png\" width=\"500\"><\/td>\n<\/tr>\n<tr>\n<td>SflaRE1Luc<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_006.png\" width=\"500\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul>\n<li>Reference<br \/>\nNakashiba, T. et al., Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab. Anim. (NY). 2023 Sep 7. doi: 10.1038\/s41684-023-01238-6. Epub ahead of print. PMID 37679611.<br \/>\nAkiyoshi R, Ogoh K, Suzuki H. 2012. Firefly luciferase. Japan patent unregistered 2012-235756<br \/>\nAkiyoshi R, Ogoh K, Suzuki H. 2013. Firefly luciferase. Japan patent unregistered 2013-74861<br \/>\nOgoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent unregistered 2013-138668<br \/>\nOgoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent unregistered 2013-81459<br \/>\nAkiyoshi R, Ogoh K, Suzuki H. 2015. FIREFLY LUCIFERASE, Patent publication US2015\/0291938 A1<\/p>\n<li>Press Release<br \/>\n<a href=\"https:\/\/web.brc.riken.jp\/en\/archives\/news\/20230908_01\">Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging.<\/a> (2023\/9\/8, RIKEN BRC)\n<\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"aluc\"><\/a><\/p>\n<h3>Artificial Luciferase Variants<\/h3>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19616<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19616\">ALuc23<\/a><\/td>\n<td>Expression vector of artificial luciferase variant ALuc23 derived from the copepod gene database for mammalian cell imaging.<\/td>\n<td>Kim, S.B. et al., 2013<\/td>\n<\/tr>\n<tr>\n<td>RDB19617<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19617\">ALuc49<\/a><\/td>\n<td>Expression vector of artificial luciferase variant ALuc49 derived from the copepod gene database for mammalian cell imaging.<\/td>\n<td>Kim, S.B. et al., 2017<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Reference<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Kim, S.B. et al. (2013) Bioconjug. Chem. 24 (12): 2067-2075. PMID 24237362<\/li>\n<li>Kim, S.B. et al. (2017) ACS Comb. Sci. 19 (9): 594-599. PMID 28742969<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<hr \/>\n<p><a name=\"bacteria\"><\/a><\/p>\n<h3>Bacterial luciferase<\/h3>\n<h4>Vibrio harveyi luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB18951<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18951\">pHLUX20<\/a><\/td>\n<td>Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain.<\/td>\n<td>Iizumi, T., 1998; Iizumi, T., 1997<\/td>\n<\/tr>\n<\/tbody>\n<table>\n<h4>Reference<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Iizumi, T. et al. (1998) Appl. Environ. Microbiol. 64 (10): 3656-3662. PMID 9758781<\/li>\n<li>Iizumi, T. and Nakamura, K. (1997) Appl. Environ. Microbiol. 63 (5): 1777-1784. PMID 9143112<\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"JNC\"><\/a><\/p>\n<h3>JNC Corporation&#8217;s Resource<\/h3>\n<h4>Aequorea  victoria Aequorin<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19836<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19836\">pAQ-B<\/a><\/td>\n<td>Expression vector of A. victoria Aequorin, wild type.<\/td>\n<td>Inouye, S. et al., 1985<\/td>\n<\/tr>\n<tr>\n<td>RDB19830<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19830\">pJN-opAQ<\/a><\/td>\n<td>Expression vector of A. victoria Aequorin, codon-optimized for human (opAQ).<\/td>\n<td>Inouye, S. et al., 1985<\/td>\n<\/tr>\n<tr>\n<td>RDB19847<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19847\">pJNC-opAQ<\/a><\/td>\n<td>Mammalian expression vector of A. victoria Aequorin, codon-optimized (opAQ).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<tr>\n<td>RDB19848<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19848\">pJNC-COsp-opAQ<\/a><\/td>\n<td>Mammalian expression vector of human cytochrome C oxidase signal peptide sequence(COsp) and A. victoria Aequorin, codon-optimized (opAQ).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Clytia gregaria ClytinI and ClytinII<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19837<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19837\">pCL41<\/a><\/td>\n<td>Expression vector of C. gregaria ClytinI, wild type.<\/td>\n<td>Inouye, S. et al., 1993<\/td>\n<\/tr>\n<tr>\n<td>RDB19838<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19838\">pCL31<\/a><\/td>\n<td>Expression vector of C. gregaria ClytinII, wild type.<\/td>\n<td>Inouye, S., 2008<\/td>\n<\/tr>\n<tr>\n<td>RDB19831<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19831\">pJN-opCLII<\/a><\/td>\n<td>Expression vector of C. gregaria ClytinII, codon-optimized for human (opCLII).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<tr>\n<td>RDB19849<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19849\">pJNC-opCLII<\/a><\/td>\n<td>Mammalian expression vector of C. gregaria ClytinII, codon-optimized (opCLII).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<tr>\n<td>RDB19850<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19850\">pJNC-COsp-opCLII<\/a><\/td>\n<td>Mammalian expression vector of human cytochrome C oxidase signal peptide sequence(COsp) and C. gregaria ClytinII, codon-optimized (opCLII).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Renilla reniformis luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19853<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19853\">pJNC-opRL<\/a><\/td>\n<td>Mammalian expression vector of R. reniformis luciferase, codon-optimized (opRLuc). <\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<tr>\n<td>RDB19854<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19854\">pJNC-opRL547<\/a><\/td>\n<td>Mammalian expression vector of R. reniformis Red-shiffted luciferase, codon-optimized (opRLuc547), 547 nm peak.<\/td>\n<td>Unpublished bioresource<\/td>\n<\/tr>\n<tr>\n<td>RDB19832<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19832\">pJN-RLBP<\/a><\/td>\n<td>Expression vector of R. reniformis luciferin-binding protein (RLBP).<\/td>\n<td>Inouye, S. et al., 2007<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Luciola cruciata luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19833<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19833\">pJN-opLcLuc<\/a><\/td>\n<td>Expression vector of L. cruciata firefly luciferase, codon-optimized for human (opLcLuc).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4> Photinus pyralis luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19834<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19834\">pJN-opPyLuc<\/a><\/td>\n<td>Expression vector of P. pyralis firefly luciferase, codon-optimized for human (opPyLuc).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Oplophorus gracilorostris luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19840<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19840\">pKAZ-412<\/a><\/td>\n<td>Expression vector of O. gracilorostris 19 kDa Protein of Oplophorus Luciferase (KAZ), catalytic component.<\/td>\n<td>Inouye, S. et al., 2000<\/td>\n<\/tr>\n<tr>\n<td>RDB19841<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19841\">pJN-eKAZ<\/a><\/td>\n<td>Expression vector of O. gracilorostris enhanced 19 kDa Protein of Oplophorus Luciferase (eKAZ).<\/td>\n<td>Inouye, S. et al., 2014<\/td>\n<\/tr>\n<tr>\n<td>RDB19851<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19851\">pJNC-eKAZ<\/a><\/td>\n<td>Mammalian expression vector of O. gracilorostris enhanced 19 kDa Protein of Oplophorus Luciferase (eKAZ).<\/td>\n<td>Inouye, S. et al., 2014<\/td>\n<\/tr>\n<tr>\n<td>RDB19852<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19852\">pJNC-GLsp-eKAZ<\/a><\/td>\n<td>Mammalian expression vector of signal peptide sequence of G. princeps Gaussia luciferase (GLsp) and O. gracilorostris enhanced 19 kDa Protein of Oplophorus Luciferase (eKAZ).<\/td>\n<td>Inouye, S. et al., 2014<\/td>\n<\/tr>\n<tr>\n<td>RDB19842<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19842\">pOL23<\/a><\/td>\n<td>Expression vector of O. gracilorostris 35 kDa protein of Oplophorus luciferase, unknown function.<\/td>\n<td>Inouye, S. et al., 2000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Gaussia princeps luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19845<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19845\">pJNC-hINS-GLuc<\/a><\/td>\n<td>Mammalian expression vector of human preproinsulin (hINS) and G. princeps Gaussia luciferase (GLuc).<\/td>\n<td>Suzuki, T. et al., 2011a<\/td>\n<\/tr>\n<tr>\n<td>RDB19846<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19846\">pJNC-hMMP2-GLuc<\/a><\/td>\n<td>Mammalian expression vector of human matrix metalloprotease-2 (hMMP2) and G. princeps Gaussia luciferase (GLuc).<\/td>\n<td>Suzuki, T. et al., 2011b<\/td>\n<\/tr>\n<tr>\n<td>RDB19855<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19855\">pJNC-pGLuc<\/a><\/td>\n<td>Mammalian expression vector of G. princeps Gaussia luciferase, codon-optimized (GLuc).<\/td>\n<td>Inouye, S. et al., 2016<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Vibrio fischeri NAD(P)H-flavin oxidoreductase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19835<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19835\">pJN-FRase<\/a><\/td>\n<td>Expression vector of V. fischeri NAD(P)H-flavin oxidoreductase (FRase).<\/td>\n<td>Inouye, S., 1994<\/td>\n<\/tr>\n<\/tbody>\n<table>\n<h4>Reference<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Inouye, S. et al. (1985) Proc. Natl. Acad. Sci. U S A. 82 (10): 3154-3158. PMID 3858813<\/li>\n<li>Inouye, S. et al. (1993) FEBS Lett. 315 (3): 343-346. PMID 8422928<\/li>\n<li>Inouye, S. (1994) FEBS Lett. 347 (2-3): 163-168. PMID 8033996<\/li>\n<li>Inouye, S. et al. (2000) FEBS Lett. 481 (1): 19-25. PMID 10984608<\/li>\n<li>Inouye, S. et al. (2007) Protein Expr. Purif. 52: 66-73. PMID 16997571<\/li>\n<li>Inouye, S. J. Biochem. 143 (5):711-717, 2008. PMID 18296715<\/li>\n<li>Inouye, S. et al. (2014)Biochem. Biophys. Res. Commun. 445 (1): 157-162. PMID 24491536<\/li>\n<li>Inouye, S. et al. (2015) Protein Expr. Purif. 109: 47-54. PMID 25665506<\/li>\n<li>Inouye, S. et al. (2016) Protein Expr. Purif. 128: 93-100. PMID 27506135<\/li>\n<li>Suzuki, T. et al. (2011a) Anal. Bichem. 415 (2): 182-189. PMID 21477579<\/li>\n<li>Suzuki, T. et al. (2011b) Plos One. 6 (9): e25243. PMID 21969874<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<hr \/>\n<p><a name=\"control\"><\/a><\/p>\n<h3>Positeve control vector<\/h3>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name of resource<\/th>\n<th>Type<\/th>\n<\/tr>\n<tr>\n<td>RDB19350<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19350\">pGL4.14-E1bTATA<\/a><br \/>\nLuciferase reporter vector of the minimal adenovirus E1b promoter sequence.<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB03988<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03988\">pCMVluc+<\/a><br \/>\nExpression vector of firefly luciferase<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB06411<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06411\">pGL3-RSV<\/a><br \/>\nExpression vector of firefly luciferase, RSV promoter<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB03989<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03989\">pCAluc+<\/a><br \/>\nExpression vector of firefly luciferase<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB02443<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02443\">pCAcc-Luc+<\/a><br \/>\nExpressing luciferase under the control of CAG promoter<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB02444<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02444\">pLRICALuc+<\/a><br \/>\nShuttle vector to generate recombinant adenovirus<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB07031<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07031\">ptk-luc<\/a><br \/>\nReporter construct with luciferase expression under tk promoter<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB02453<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02453\">AxCA Luc+<\/a><br \/>\nRecombinant adenovirus expressing luciferase gene<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB04026<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB04026\">pKM2L<\/a><br \/>\nReporter construct with Renilla luciferase gene<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB05549<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB05549\">pKM2L-pvTK<\/a><br \/>\nHSV herpes simplex virus thymidine kinase (HSV-TK)<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB05550<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB05550\">pKM2L-pvSV40<\/a><br \/>\nSV40 simian virus 40 early enhancer\/promoter region (SV40)<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB05551<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB05551\">pKM2L-pvCMV<\/a><br \/>\nCMV cytomegalovirus immediate enhancer\/promoter (CMV)<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB06408<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06408\">pRL-CMV mut1<\/a><br \/>\nExpression vector of Renilla luciferase, mutated CMV promoter<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB06409<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06409\">pRL-CMV mut2<\/a><br \/>\nExpression vector of Renilla luciferase, mutated CMV promoter<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB06410<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06410\">pRL-RSV<\/a><br \/>\nExpression vector of Renilla luciferase, RSV promoter<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<hr \/>\n<p style=\"text-align: left;\">2025.06.03 (N.N. GRP0025e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Luciferase Akaluc luciferase providing brighter and red-shifted luminescence Brighter than commonly used luciferase! Artificial Luciferase Variants Bacterial luciferase JNC Corporation&#8217;s Resource Positeve control vector Akaluc luciferase providing brighter and red-shifted luminescence Firefly bioluminescence systems have been commonly used as a imaging tool of biological phenomena. However, it have not been strong enough for imaging signals in deep tissues, because of low permeabilization of substrates. Recently, Dr. Atsushi Miyawaki and Dr. Satoshi Iwano of the RIKEN Center for Brain Science, and Dr. Shojiro Maki of the University of Electro-Communications developed a system of artificial bioluminescence AkaBLI that enables noninvasive signal observation in deep tissue of living animals. The AkaBLI system consists of an artificial substrate AkaLumine with improved tissue permeability and an artificial luciferase Akaluc optimized to AkaLumine. The intensity of the luminescence of AkaBLI system is 100 to 1000 folds brighter than the conventional systems. The expression vector of artificial luciferase Akaluc has been deposited by Dr. Miyawaki&#8217;s lab and it is now available from the DNA Bank. Reference Nakashiba, T. et al., Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab. Anim. (NY). 2023 Sep 7. doi: [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8551,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8565","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8565","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8565"}],"version-history":[{"count":3,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8565\/revisions"}],"predecessor-version":[{"id":9007,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8565\/revisions\/9007"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8551"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8565"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}