{"id":8544,"date":"2025-05-03T11:07:16","date_gmt":"2025-05-03T02:07:16","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8544"},"modified":"2025-06-05T17:30:43","modified_gmt":"2025-06-05T08:30:43","slug":"tetsystemen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/regurationen\/tetsystemen","title":{"rendered":"Tetracycline inducible system"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><br \/>\n<a name=\"tet\"><\/a><\/p>\n<h2>Tetracycline inducible system<\/h2>\n<h3>Lentivirus vector<\/h3>\n<h4>Tet-inducible shRNA expression<\/h4>\n<table>\n<tbody>\n<tr>\n<th><span style=\"color: #000080;\">Cat. no.<\/span><\/th>\n<th><span style=\"color: #000080;\">Plasmid Name<\/span><\/th>\n<th><span style=\"color: #000080;\">Description<\/span><\/th>\n<th><span style=\"color: #000080;\">Data sheet<\/span><\/th>\n<\/tr>\n<tr>\n<td>RDB07916<\/td>\n<td>pENTR4-H1tetOx1<\/td>\n<td>Gateway entry vector, Human H1 promoter, tet operator<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07916\">[Data sheet]<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB07917<\/td>\n<td>CS-RfA-ETBsd<\/td>\n<td>Gateway, EF-1\u03b1 promoter, TetR-2A-Blasticidin<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07917\">[Data sheet]<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB08020<\/td>\n<td>CS-RfA-ETV<\/td>\n<td>Gateway, EF-1\u03b1 promoter, TetR-2A-Venus<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08020\">[Data sheet]<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB08362<\/td>\n<td>CS-RfA-ETR<\/td>\n<td>Gateway, EF-1\u03b1 promoter, TetR-2A-mRFP1<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08362\">[Data sheet]<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Tet-inducible cDNA expression<\/h4>\n<table>\n<tbody>\n<tr>\n<th><span style=\"color: #000080;\">Cat. no.<\/span><\/th>\n<th><span style=\"color: #000080;\">Plasmid Name<\/span><\/th>\n<th><span style=\"color: #000080;\">Description<\/span><\/th>\n<th><span style=\"color: #000080;\">Data sheet<\/span><\/th>\n<\/tr>\n<tr>\n<td>RDB12876<\/td>\n<td>CSIV-TRE-RfA-CMV-KT<\/td>\n<td>Tet-responsive promoter, Gateway, CMV promoter, hKO1-2A-rtTA, loxP<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12876\">[Data sheet]<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB12878<\/td>\n<td>CSIV-TRE-RfA-UbC-KT<\/td>\n<td>Tet-responsive promoter, Gateway, UbC promoter, hKO1-2A-rtTA, loxP<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12878\">[Data sheet]<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB12872<\/td>\n<td>CSIV-RfA-TRE-EF-KT<\/td>\n<td>Tet-responsive promoter, Gateway, EF-1\u03b1 promoter, hKO1-2A-rtTA, loxP<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12872\">[Data sheet]<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Tet-inducible GFP expression<\/h4>\n<table>\n<tbody>\n<tr>\n<th><span style=\"color: #000080;\">Cat. no.<\/span><\/th>\n<th><span style=\"color: #000080;\">Plasmid Name<\/span><\/th>\n<th><span style=\"color: #000080;\">Description<\/span><\/th>\n<th><span style=\"color: #000080;\">Data sheet<\/span><\/th>\n<\/tr>\n<tr>\n<td>RDB12875<\/td>\n<td>CSIV-TRE-GFP-CMV-KT<\/td>\n<td>Tet-responsive promoter, EGFP, CMV promoter, hKO1-2A-rtTA, loxP<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12875\">[Data sheet]<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB12877<\/td>\n<td>CSIV-TRE-GFP-UbC-KT<\/td>\n<td>Tet-responsive promoter, EGFP, UbC promoter, hKO1-2A-rtTA, loxP<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12877\">[Data sheet]<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB12871<\/td>\n<td>CSIV-GFP-TRE-EF-KT<\/td>\n<td>Tet-responsive promoter, EGFP, EF-1\u03b1 promoter, hKO1-2A-rtTA, loxP<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12871\">[Data sheet]<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Plasmid vector<\/h3>\n<h4>Vector backbone<\/h4>\n<table>\n<tbody>\n<tr>\n<td>RDB09232<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB09232\">pBlKS hCMV*-1 b-globinpA<\/a><br \/>Expression vector of tet-inducible CMV promoter b-globin poly(A) casette.<\/td>\n<\/tr>\n<tr>\n<td>RDB06012<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06012\">pAdex TRE-SwaI<\/a><br \/>Shuttle vector to generate rAd with Tet-responsive element (TRE) promoter<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4>Tet regulatory protein expression<\/h4>\n<table>\n<tbody>\n<tr>\n<td>RDB18685<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18685\">pAAV2-SYN-tTAad-BGHpA<\/a><br \/>AAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I.<\/td>\n<\/tr>\n<tr>\n<td>RDB15056<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15056\">pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova)<\/a><br \/>Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed.<\/td>\n<\/tr>\n<tr>\n<td>RDB15055<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15055\">pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova)<\/a><br \/>Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter. <\/td>\n<\/tr>\n<tr>\n<td>RDB15047<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15047\">pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)<\/a><br \/>Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter. <\/td>\n<\/tr>\n<tr>\n<td>RDB15045<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15045\">pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)<\/a><br \/>Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter.<\/td>\n<\/tr>\n<tr>\n<td>RDB13212<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13212\">pAAV-SP-FtTA<\/a><br \/>AAV vector of  FLAG-tTA under the control of Substance P promoter<\/td>\n<\/tr>\n<tr>\n<td>RDB13211<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13211\">pAAV-Enk-FtTA<\/a><br \/>AAV vector of  FLAG-tTA under the control of Enkephalin promoter<\/td>\n<\/tr>\n<tr>\n<td>RDB12109<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12109\">pBRPyCAG-tTAFF-IN<\/a><\/td>\n<\/tr>\n<tr>\n<td>RDB02351<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02351\">Ax1 CM-NtTA<\/a><br \/>Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal<\/td>\n<\/tr>\n<tr>\n<td>RDB01733<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01733\">Ax1 CM-NtTA<\/a><br \/>Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal<\/td>\n<\/tr>\n<tr>\n<td>RDB01732<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01732\">Ax1-CA-NtTA<\/a><br \/>Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal<\/td>\n<\/tr>\n<tr>\n<td>RDB01731<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01731\">Ax1-Tet NtTA<\/a><br \/>Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal<\/td>\n<\/tr>\n<tr>\n<td>RDB01730<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01730\">Ax1-RxNtTA<\/a><br \/>Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal<\/td>\n<\/tr>\n<tr>\n<td>RDB01711<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01711\">pAx1-CM-NtTA<\/a><br \/>Shuttle vector to generate rAd expressing NtTA<\/td>\n<\/tr>\n<tr>\n<td>RDB01710<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01710\">pAx1-CA-NtTA<\/a><br \/>Shuttle vector to generate rAd expressing NtTA<\/td>\n<\/tr>\n<tr>\n<td>RDB01709<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01709\">pAx1-Tet NtTA<\/a><br \/>Shuttle vector to generate rAd expressing NtTA<\/td>\n<\/tr>\n<tr>\n<td>RDB01708<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01708\">pAx1-RxNtTA<\/a><br \/>Recombinant adenoviral vector expressing NtTA (Tet-transactivator)<\/td>\n<\/tr>\n<tr>\n<td>RDB01684<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01684\">pCM-NtTA<\/a><br \/>CM-NtTA (Tet-transactivator driven by CM promoter)<\/td>\n<\/tr>\n<tr>\n<td>RDB01683<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01683\">pTet NtTA<\/a><br \/>Tet-NtTA (Tet-transactivator driven by tet promoter)<\/td>\n<\/tr>\n<tr>\n<td>RDB01583<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01583\">pAxCMtTA<\/a><br \/>Shuttle vector to generate rAd expressing E. coli Tet-inducible tTA gene<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4>Positive control vector<\/h4>\n<table>\n<tbody>\n<tr>\n<td>RDB01584<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01584\">pAxTetZ<\/a><br \/>Shuttle vector to generate rAd expressing tet-inducible lacZ<\/td>\n<\/tr>\n<tr>\n<td>RDB01574<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01574\">AxTetZ<\/a><br \/>Recombinant adenovirus expressing Tet-inducible lacZ gene<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<p style=\"text-align:left;\">2025.06.03 (T.M. GRP0085e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tetracycline inducible system Lentivirus vector Tet-inducible shRNA expression Cat. no. Plasmid Name Description Data sheet RDB07916 pENTR4-H1tetOx1 Gateway entry vector, Human H1 promoter, tet operator [Data sheet] RDB07917 CS-RfA-ETBsd Gateway, EF-1\u03b1 promoter, TetR-2A-Blasticidin [Data sheet] RDB08020 CS-RfA-ETV Gateway, EF-1\u03b1 promoter, TetR-2A-Venus [Data sheet] RDB08362 CS-RfA-ETR Gateway, EF-1\u03b1 promoter, TetR-2A-mRFP1 [Data sheet] Tet-inducible cDNA expression Cat. no. Plasmid Name Description Data sheet RDB12876 CSIV-TRE-RfA-CMV-KT Tet-responsive promoter, Gateway, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet] RDB12878 CSIV-TRE-RfA-UbC-KT Tet-responsive promoter, Gateway, UbC promoter, hKO1-2A-rtTA, loxP [Data sheet] RDB12872 CSIV-RfA-TRE-EF-KT Tet-responsive promoter, Gateway, EF-1\u03b1 promoter, hKO1-2A-rtTA, loxP [Data sheet] Tet-inducible GFP expression Cat. no. Plasmid Name Description Data sheet RDB12875 CSIV-TRE-GFP-CMV-KT Tet-responsive promoter, EGFP, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet] RDB12877 CSIV-TRE-GFP-UbC-KT Tet-responsive promoter, EGFP, UbC promoter, hKO1-2A-rtTA, loxP [Data sheet] RDB12871 CSIV-GFP-TRE-EF-KT Tet-responsive promoter, EGFP, EF-1\u03b1 promoter, hKO1-2A-rtTA, loxP [Data sheet] Plasmid vector Vector backbone RDB09232 pBlKS hCMV*-1 b-globinpAExpression vector of tet-inducible CMV promoter b-globin poly(A) casette. RDB06012 pAdex TRE-SwaIShuttle vector to generate rAd with Tet-responsive element (TRE) promoter &nbsp; Tet regulatory protein expression RDB18685 pAAV2-SYN-tTAad-BGHpAAAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I. RDB15056 pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova)Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8516,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8544","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8544","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8544"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8544\/revisions"}],"predecessor-version":[{"id":9002,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8544\/revisions\/9002"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8516"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8544"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}