{"id":8533,"date":"2025-05-03T11:02:13","date_gmt":"2025-05-03T02:02:13","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8533"},"modified":"2026-03-31T08:32:24","modified_gmt":"2026-03-30T23:32:24","slug":"creloxpen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/regurationen\/creloxpen","title":{"rendered":"Cre-loxP"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><br \/>\n<a name=\"cre\"><\/a><\/p>\n<h2>Conditional Gene Expression<\/h2>\n<h3>Cre-loxP system<\/h3>\n<h4>Vector backbone<\/h4>\n<table>\n<tbody>\n<tr>\n<td>RDB15114<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15114\">pBSIISK-hFLEX<\/a><br \/>Plasmid clone of Cre recombinase mediated flip-excision (FLEX) switch.<\/td>\n<\/tr>\n<tr>\n<td>RDB04397<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB04397\">pUSwS<\/a><br \/>A cassette plasmid for inserting DNA between two loxP S sequences in the same orientation<\/td>\n<\/tr>\n<tr>\n<td>RDB04396<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB04396\">pUVwV<\/a><br \/>A cassette plasmid for inserting DNA between two loxP V sequences in the same orientation<\/td>\n<\/tr>\n<tr>\n<td>RDB03446<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03446\">pUAd5LCALNL(W1975C23R)<\/a><br \/>Shuttle vector to generate recombinant adenovirus<\/td>\n<\/tr>\n<tr>\n<td>RDB01862<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01862\">pCALNL5<\/a><br \/>A cassette to introduce cDNA into mammalian chromosome<\/td>\n<\/tr>\n<tr>\n<td>RDB01679<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01679\">pCALwL<\/a><br \/>A cassette plasmid to insert cDNA into downstream CAG promoter<\/td>\n<\/tr>\n<tr>\n<td>RDB01677<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01677\">pULwL<\/a><br \/>A cassette plasmid to insert stuffer DNA between two loxP site<\/td>\n<\/tr>\n<tr>\n<td>RDB01674<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01674\">pCALNLw<\/a><br \/>A cassette to introduce cDNA into mammalian chromosome<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4>Cre expression<\/h4>\n<table>\n<tbody>\n<tr>\n<td>RDB20979<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB20979\">pscAAV-CAG-Cre<\/a><br \/>Recombinant AAV vector packaging self-complementary Cre under the CAG promoter.<\/td>\n<\/tr>\n<tr>\n<td>RDB16797<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16797\">pAcGFP-NCre<\/a><br \/>Mammalian expression vector encoding Cre recombinase tagged with AcGFP1.<\/td>\n<\/tr>\n<tr>\n<td>RDB15053<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15053\">pK073.TRE-Dre-WPRE (Supernova)<\/a><br \/>Dre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Dre recombinase. <\/td>\n<\/tr>\n<tr>\n<td>RDB15050<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15050\">pK170.AAV-TRE-Cre-WPRE (Supernova)<\/a><br \/>Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. <\/td>\n<\/tr>\n<tr>\n<td>RDB15043<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15043\">pK031.TRE-Cre (Supernova)<\/a><br \/>Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. <\/td>\n<\/tr>\n<tr>\n<td>RDB14533<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14533\">pCS2-OlCre<\/a><br \/>Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka).<\/td>\n<\/tr>\n<tr>\n<td>RDB12117<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12117\">pPyCAG-CreGR-IB<\/a><br \/>Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. Blasticidin S resistance.<\/td>\n<\/tr>\n<tr>\n<td>RDB09596<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB09596\">pPyCAG-Cre-IRESpuropA<\/a><br \/>Expression vector of Cre recombinase. Puromycin resistance.<\/td>\n<\/tr>\n<tr>\n<td>RDB08998<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08998\">pCAGGS-Cre<\/a><br \/>Expression clone of Cre recombinase.<\/td>\n<\/tr>\n<tr>\n<td>RDB07106<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07106\">pPyCAG-CreGR-ID<\/a><br \/>Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. HisD resistance.<\/td>\n<\/tr>\n<tr>\n<td>RDB06073<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06073\">pKM2C<\/a><br \/>Reporter construct with Cre recombinase gene<\/td>\n<\/tr>\n<tr>\n<td>RDB01748<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01748\">AxCANCre<\/a><br \/>Recombinant adenovirus expressing recombinase Cre with nuclear localization signal<\/td>\n<\/tr>\n<tr>\n<td>RDB01675<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01675\">pxCANCre<\/a><br \/>A plasmid expressing the recombinase Cre tagged with a nls<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4>Positive control vector<\/h4>\n<p>Plasmid clone <a href=\"https:\/\/brc.riken.jp\/dna\/RDB06232\">pxCALNLEYFP (RDB06232)<\/a> can be used for a positive control of Cre-mediated gene regulation. The pxCALNLEYFP contains CAG promoter, loxP-Neo-loxP stuffer sequence and coding region of EYFP. In this example, the pxCALNLEYFP was transfected to COS1 cells with or without <a href=\"https:\/\/brc.riken.jp\/dna\/RDB01675\">pxCANCre (RDB01675)<\/a>, which expresses Cre recombinase.<br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sugano1.png\" \/><br \/>\nExpression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).<\/p>\n<table>\n<tbody>\n<tr>\n<td>RDB16747<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16747\">pCALNL5:mCherry<\/a><br \/>Cre recombinase inducible expression cassette of mCherry.<\/td>\n<\/tr>\n<tr>\n<td>RDB06232<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06232\">pxCALNLEYFP<\/a><br \/>Expression vector of EYFP<\/td>\n<\/tr>\n<tr>\n<td>RDB03347<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03347\">AxCALNLEGFP (forward)<\/a><br \/>Recombinant adenovirus harboring enhanced green fluorescent protein cDNA<\/td>\n<\/tr>\n<tr>\n<td>RDB03262<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03262\">pAxCALNLEYFP (reverse)<\/a><br \/>Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA<\/td>\n<\/tr>\n<tr>\n<td>RDB03261<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03261\">pAxCALNLEYFP (forward)<\/a><br \/>Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA.<\/td>\n<\/tr>\n<tr>\n<td>RDB03260<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03260\">pAxCALNLEGFP (reverse)<\/a><br \/>Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA<\/td>\n<\/tr>\n<tr>\n<td>RDB03259<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03259\">pAxCALNLEGFP (forward)<\/a><br \/>Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA.<\/td>\n<\/tr>\n<tr>\n<td>RDB03252<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03252\">pAxCALNLBFP (reverse)<\/a><br \/>Shuttle vector for rAd harboring blue fluorescent protein cDNA<\/td>\n<\/tr>\n<tr>\n<td>RDB03251<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03251\">pAxCALNLBFP (forward)<\/a><br \/>Shuttle vector for rAd harboring blue fluorescent protein cDNA.<\/td>\n<\/tr>\n<tr>\n<td>RDB01750<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01750\">AxCALNLNZ<\/a><br \/>Recombinant adenovirus expressing E. coli lacZ<\/td>\n<\/tr>\n<tr>\n<td>RDB01680<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01680\">pCALNLZ<\/a><br \/>A control plasmid to introduce LacZ gene into mammalian chromosome<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<p style=\"text-align:left;\">2026.03.31 (T.M. GRP0086e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Conditional Gene Expression Cre-loxP system Vector backbone RDB15114 pBSIISK-hFLEXPlasmid clone of Cre recombinase mediated flip-excision (FLEX) switch. RDB04397 pUSwSA cassette plasmid for inserting DNA between two loxP S sequences in the same orientation RDB04396 pUVwVA cassette plasmid for inserting DNA between two loxP V sequences in the same orientation RDB03446 pUAd5LCALNL(W1975C23R)Shuttle vector to generate recombinant adenovirus RDB01862 pCALNL5A cassette to introduce cDNA into mammalian chromosome RDB01679 pCALwLA cassette plasmid to insert cDNA into downstream CAG promoter RDB01677 pULwLA cassette plasmid to insert stuffer DNA between two loxP site RDB01674 pCALNLwA cassette to introduce cDNA into mammalian chromosome &nbsp; Cre expression RDB20979 pscAAV-CAG-CreRecombinant AAV vector packaging self-complementary Cre under the CAG promoter. RDB16797 pAcGFP-NCreMammalian expression vector encoding Cre recombinase tagged with AcGFP1. RDB15053 pK073.TRE-Dre-WPRE (Supernova)Dre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Dre recombinase. RDB15050 pK170.AAV-TRE-Cre-WPRE (Supernova)Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. RDB15043 pK031.TRE-Cre (Supernova)Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8516,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8533","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8533","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8533"}],"version-history":[{"count":5,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8533\/revisions"}],"predecessor-version":[{"id":9136,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8533\/revisions\/9136"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8516"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8533"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}