{"id":8516,"date":"2025-05-03T10:52:36","date_gmt":"2025-05-03T01:52:36","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8516"},"modified":"2025-05-03T10:53:23","modified_gmt":"2025-05-03T01:53:23","slug":"regurationen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/regurationen","title":{"rendered":"Genome editing &amp; Expression Regulation"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>Expression regulation<\/h2>\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"https:\/\/dna.brc.riken.jp\/en\/regurationen\/crisperen\">Genome Editing by CRISPR\/Cas9<\/a>\n<ul style=\"line-height: 1.5em;\">\n<li>Expression vector of Cas9 enzyme<\/li>\n<li>Plasmids for the evaluation of the efficiency of genome editing<\/li>\n<li>Knock-in markers with CRISPR\/Cas9 genome editing<\/li>\n<div align=\"center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB13130_01.png\" width=\"550\" \/>\n<\/div>\n<\/ul>\n<\/li>\n<li><a href=\"https:\/\/dna.brc.riken.jp\/en\/regurationen\/auxindegronen\">Auxin Inducible Degron (AID) System<\/a>\n<ul style=\"line-height: 1.5em;\">\n<li>Using the AID System, you can degrade proteins fused with a degron (a small domain that can induce protein degradation) in a very short period of time.<\/li>\n<\/ul>\n<\/li>\n<li><a href=\"https:\/\/dna.brc.riken.jp\/en\/regurationen\/creloxpen\">Cre-loxP system<\/a><\/li>\n<li><a href=\"https:\/\/dna.brc.riken.jp\/en\/regurationen\/flpfrten\">FLP-FRT system<\/a><\/li>\n<li><a href=\"https:\/\/dna.brc.riken.jp\/en\/regurationen\/tetsystemen\">Tet inducible system<\/a><\/li>\n<\/ul>\n<hr \/>\n<p style=\"text-align: left;\">2025.05.03 (T.M. GRP0073e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Expression regulation Genome Editing by CRISPR\/Cas9 Expression vector of Cas9 enzyme Plasmids for the evaluation of the efficiency of genome editing Knock-in markers with CRISPR\/Cas9 genome editing Auxin Inducible Degron (AID) System Using the AID System, you can degrade proteins fused with a degron (a small domain that can induce protein degradation) in a very short period of time. Cre-loxP system FLP-FRT system Tet inducible system 2025.05.03 (T.M. GRP0073e)<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8516","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8516","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8516"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8516\/revisions"}],"predecessor-version":[{"id":8520,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8516\/revisions\/8520"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8516"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}