{"id":8478,"date":"2025-05-03T08:47:45","date_gmt":"2025-05-02T23:47:45","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8478"},"modified":"2025-06-16T17:59:44","modified_gmt":"2025-06-16T08:59:44","slug":"thermoen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/researchen\/thermoen","title":{"rendered":"Thermophile Gene Expression Plasmid"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h3>Outline<\/h3>\n<p><i>Aeropyrum pernix<\/i>, <i>Pyrococcus horikoshii<\/i>, <i>Sulfolobus tokodaii<\/i> and <i>Thermus thermophilus<\/i> gene expression plasmids and <i>T thermophilus<\/i> gene disruption plasmids were deposited by Dr.Yutaka Kawarabayasi, National Institute of Advanced Industrial Science and Dr. Seiki Kuramitsu, Osaka University. <\/p>\n<hr \/>\n<ul>\n<li>Plasmids for producing recombinant protein of thermophile in <i>E. coli<\/i>.\n<ul>\n<li><a href=\"\/en\/researchen\/thermoen\/thermo_ape\"><i>Aeropyrum pernix<\/i><\/a><\/li>\n<li><a href=\"\/en\/researchen\/thermoen\/thermo_pho\"><i>Pyrococcus horikoshii<\/i><\/a><\/li>\n<li><a href=\"\/en\/researchen\/thermoen\/thermo_sto\"><i>Sulfolobus tokodaii<\/i><\/a><\/li>\n<li><a href=\"\/en\/researchen\/thermoen\/thermo_tth\"><i>Thermus thermophilus<\/i><\/a><\/li>\n<\/ul>\n<\/li>\n<li>Plasmids for gene disruption of <a href=\"\/en\/researchen\/thermoen\/thermo_tth\"><i>Thermus thermophilus<\/i><\/a>.<\/li>\n<li>Plasmids are shipped as DNA solution in TE buffer in individual tube.<\/li>\n<li>Please visit Informaion site <img decoding=\"async\" alt=\"data sheet\" src=\"https:\/\/dnaconda.riken.jp\/images\/datasheet097.gif\" width=\"30\" \/> for articles published by using this materials, references and tips.\n<ul>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07353\"><i>Aeropyrum pernix<\/i> deposited by Dr. Kuramitsu <\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07352\"><i>Sulfolobus tokodaii<\/i> deposited by Dr. Kuramitsu<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15969\">Thermophile Gene Expression Plasmid deposited by Dr. Kawarabayasi<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3>How to obtain plasmids<\/h3>\n<p>Please specify Catalog number and Plasmid ID (ex. THR041204, StoEx03A04).<br \/>\nPlease indicate Terms and Conditions for Distribution in MTA as follows:<\/p>\n<ul>\n<li>In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER is expected to cite the literature(s) specified by the DEPOSITOR.<\/li>\n<\/ul>\n<p><!-- Begin of Form Menu --><br \/>\n\n<table id=\"tablepress-13\" class=\"tablepress tablepress-id-13\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\">Order Form:<br \/>Please complete the form with your shipping information including your account number of an international courier<br \/>(FedEx. 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[Word]<\/a>&nbsp;[<a href=\"https:\/\/brc.riken.jp\/dna\/frmeexord\">Example of order form<\/a>]<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-13 from cache --><br \/>\n\n<table id=\"tablepress-15\" class=\"tablepress tablepress-id-15\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><a href=\"https:\/\/brc.riken.jp\/dna\/frmecad\">Material Transfer Agreement (Category I MTA) [Word]<\/a><br \/><br \/>\nFor the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization.<br \/><ul><li>Regarding Section 2(a):<br \/>Please write your research purpose in detail. We need description specifically how and for what purpose you are going to use the DNA Bank resource(s). If the information is considered insufficient, we may ask you to add more or rewrite it.<br \/>We can check whether the documents are filled out correctly or not in advance. 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Therefore, we ask your Authorized Representative to be an authority listed above.<\/li><\/ul><\/td><td class=\"column-2\"><a href=\"https:\/\/brc.riken.jp\/dna\/frmecpd\">Material Transfer Agreement (Category II MTA) [Word]<\/a><br \/><br \/>\nFor the use of our bioresource in research for the following cases:<ul><li>For research to be conducted by for-profit organizations.<\/li><li>For collaborative research between for-profit organization and not-for-profit organization.<\/li><li>For research by not-for- organization outsourced and sponsored by for-profit organization.<\/li><li>For for-profit research by not-for-profit organization including R&amp;D with the aim of patent acquisition.<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-15 from cache --><br \/>\nSend forms:<br \/>\n\n<table id=\"tablepress-1\" class=\"tablepress tablepress-id-1\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\">The DNA Bank, RIKEN BioResource Research Center (BRC),<br \/>\n3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan<br \/>\n E-mail: &#100;&#110;&#97;&#95;&#115;&#101;&#99;&#46;&#98;&#114;&#99;&#64;&#114;&#105;&#107;&#101;&#110;&#46;&#106;&#112;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-1 from cache --><\/p>\n<p>Please visit <a href=\"\/en\/orderingen\">further information of distribution and fees<\/a>.<\/p>\n<hr \/>\n<p style=\"text-align: left;\">2025.06.15 (T.M. GRP0062e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Outline Aeropyrum pernix, Pyrococcus horikoshii, Sulfolobus tokodaii and Thermus thermophilus gene expression plasmids and T thermophilus gene disruption plasmids were deposited by Dr.Yutaka Kawarabayasi, National Institute of Advanced Industrial Science and Dr. Seiki Kuramitsu, Osaka University. Plasmids for producing recombinant protein of thermophile in E. coli. Aeropyrum pernix Pyrococcus horikoshii Sulfolobus tokodaii Thermus thermophilus Plasmids for gene disruption of Thermus thermophilus. Plasmids are shipped as DNA solution in TE buffer in individual tube. Please visit Informaion site for articles published by using this materials, references and tips. Aeropyrum pernix deposited by Dr. Kuramitsu Sulfolobus tokodaii deposited by Dr. Kuramitsu Thermophile Gene Expression Plasmid deposited by Dr. Kawarabayasi How to obtain plasmids Please specify Catalog number and Plasmid ID (ex. THR041204, StoEx03A04). Please indicate Terms and Conditions for Distribution in MTA as follows: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER is expected to cite the literature(s) specified by the DEPOSITOR. Send forms: Please visit further information of distribution and fees. 2025.06.15 (T.M. GRP0062e)<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8163,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8478","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8478"}],"version-history":[{"count":4,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8478\/revisions"}],"predecessor-version":[{"id":9020,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8478\/revisions\/9020"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8163"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}