{"id":8471,"date":"2025-05-03T08:41:12","date_gmt":"2025-05-02T23:41:12","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8471"},"modified":"2025-05-03T08:45:48","modified_gmt":"2025-05-02T23:45:48","slug":"pombeorfen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/researchen\/pombeorfen","title":{"rendered":"S. pombe ORFeome plasmid"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><br \/>\n<i>Schizosaccharomyces pombe<\/i> ORFeome plasmids were deposited by Dr. Minoru Yoshida, Chemical Genetics Lagoratory, RIKEN; These consists of 4,900 entry plasmids. Plasmids are provided individually.<\/p>\n<p><a href=\"http:\/\/www.riken.jp\/~\/media\/riken\/pr\/press\/2006\/20060626_1\/20060626_1_en.pdf\">Establishing an entire protein assay system of &#8220;fission yeast&#8221;: Trends from genetic information to entire products in genomic analysis [2006.06.26 RIKEN Press release (pdf)]<\/a><br \/>\nLocalization data is available at<br \/>\n<a href=\"http:\/\/www.riken.jp\/SPD\/index.html\">S. pombe Postgenome Database<\/a><\/p>\n<ul>\n<li>The <i>Schizosaccharomyces pombe<\/i> Postgenome Database (SPD) contains the results of systematic analysis of protein expression and localization based on fission yeast ORFeome cloning as well as basic information on genes and proteins.<\/li>\n<li>The ORF collection currently covers 99.2% of the fission yeast protein-coding genes and pseudogenes that are reported by the Wellcome Trust Sanger Institute.<\/li>\n<\/ul>\n<h3>Distribution of plasmids<\/h3>\n<p><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0054j\">in Japanese<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/jpn.gif\" border=\"0\" \/><\/a><\/p>\n<h4>1. . Distribution of plasmids<\/h4>\n<h5>1.1 Terms and conditions for distribution<\/h5>\n<p>In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must cite the following literature(s). (Matsuyama et al., 2006, Nature Biotech., 24, 841-847.).<br \/>\nIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must state an acknowledgement to the DEPOSITOR.<br \/>\nWhen the USER wishes to sell the BIOLOGICAL RESOURCE and\/or its DERIVATIVES on commercial basis, the USER must obtain a prior written permission from the DEPOSITOR .<br \/>\nWhen requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the USER&#8217;s name and his\/her affiliation.<\/p>\n<h5>1.2 How to obtain plasmids <\/h5>\n<p>Plasmids are shipped as DNA solution in TE buffer in an individual tube.<\/p>\n<h5><span style=\"text-decoration: underline;\">Order Forms<\/span><\/h5>\n<p>Please specify ID of individual plasmids.<\/p>\n<form action=\"https:\/\/knowledge.brc.riken.jp\/resource\/dna\/list\" target=\"_blank\" accept-charset=\"utf-8\"><input name=\"query2\" type=\"hidden\" value=\"pombe\"><input type=\"search\" class=\"field\" name=\"query\" placeholder=\"gene name\"><input  type=\"hidden\" name=\"__lang__\" value=\"ja\"><button class=\"button\"  type=\"submit\">Search<\/button>\n<\/form>\n<p>There are three kinds of plasmids as follows.<br \/>\nEntry plasmid (SpENT), Cat#. RDB 5820<br \/>\nFLAG2-His6-tagged ORF (SpFFH), Cat#. RDB 6106<br \/>\nYFP-FLAG-His6-tagged ORF (SpYFH), Cat#. RDB 6107<br \/>\nPlease reffer <a href=\"#datasheet\">Data Sheet <img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/datasheet1.png\" width=\"145\" height=\"30\" \/><\/a> for datail.<\/p>\n<div class=\"text02j\">Please write plasmid ID in FormA, for example,<br \/>\n01\/A01 SPBC1711.15c (SpENT)<br \/>\n01\/A01 SPBC1711.15c (SpYFH)<br \/>\n01\/B11 SPAC16A10.05c (SpYFH)<br \/>\n01\/E01 SPCC191.04c (SpFFH)\n<\/div>\n<ul>\n<li>Order Form<br \/>\n\n<table id=\"tablepress-13\" class=\"tablepress tablepress-id-13\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\">Order Form:<br \/>Please complete the form with your shipping information including your account number of an international courier<br \/>(FedEx. World Courier, TNT Express, DHL Global Forwarding and others).<br \/>See detail in <a href=\"\/en\/orderingen\" target=\"_blank\" rel=\"noopener noreferrer\">Information of Request for Distribution<\/a><br \/><br \/>Note:<br \/>The resident of the European Economic Area (EEA) and China, please read <a href=\"\/en\/orderingen#sdirfc\">Special distribution Information to Residents of the Foreign Countries<\/a><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><a href=\"https:\/\/brc.riken.jp\/dna\/frmeacrd\">Order Form for Credit Card Payment. (Visa or Master Card only) [Word]<\/a>&nbsp;[<a href=\"https:\/\/brc.riken.jp\/dna\/frmeexord\">Example of order form<\/a>]<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><a href=\"https:\/\/brc.riken.jp\/dna\/frmead\">Order Form for Bank Transfer Payment. [Word]<\/a>&nbsp;[<a href=\"https:\/\/brc.riken.jp\/dna\/frmeexord\">Example of order form<\/a>]<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-13 from cache -->\n<\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/frmecaspdd\">Material Transfer Agreement<\/a> for S. pombe ORFeome plasmids:<br \/>\nWe would like to ask a signature of the Authorized Representative of a recipient institution.<\/li>\n<li>Regarding description of specificpurpose in the Section 2(a):<br \/>\nPlease write your research purpose in detail. We need description specifically how and for what purpose you are going to use the DNA Bank resource(s). If the information is considered insufficient, we may ask you to add more or rewrite it.<br \/>\nWe can check whether the documents are filled out correctly or not in advance. Please email documents to us.<\/li>\n<\/ul>\n<p>Please visit <a href=\"\/en\/orderingen\">Ordering Information<\/a> for further information of distribution and fees.<\/p>\n<p>Send forms by regular mail or scanned PDF by e-mail:<\/p>\n<div class=\"text02j\" style=\"padding-left: 60px;\">The DNA Bank, RIKEN BioResource Research Center,<br \/>\n3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan<br \/>\nE-mail: &#100;&#110;&#97;&#98;&#97;&#110;&#107;&#46;&#98;&#114;&#99;&#64;&#114;&#105;&#107;&#101;&#110;&#46;&#106;&#112;<\/p>\n<\/div>\n\n<table id=\"tablepress-20\" class=\"tablepress tablepress-id-20\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><u>Distribution fee per clone<\/u>:<br \/>Please refer <a href=\"https:\/\/dna.brc.riken.jp\/en\/orderingen\/order_feeen\">Fee and Payment<\/a><ul><li>The cost of shipping containers, dry ice and freight will be charged separately. These are not included in the fee.<\/li><li>For details for fee and payment, please visit <a href=\"\/en\/orderingen\">Information of Request for Distribution<\/a>.<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-20 from cache -->\n<p><a name=\"datasheet\"><\/a><\/p>\n<h3>DATA SHEET&nbsp;<img loading=\"lazy\" decoding=\"async\" alt=\"data sheet\" src=\"https:\/\/dnaconda.riken.jp\/images\/datasheet1.png\" width=\"145\" height=\"30\" \/><\/h3>\n<table border=\"0\" width=\"700\" cellspacing=\"0\" cellpadding=\"4\">\n<tbody>\n<tr>\n<td colspan=\"2\">1. <i>Schizosaccharomyces pombe<\/i> ORFeome plasmids<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\">1.2 Plasmid information<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\">\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"4\">\n<tbody>\n<tr>\n<td colspan=\"2\">Entry plasmids<\/td>\n<\/tr>\n<tr>\n<td>Vector<\/td>\n<td>pDONR201 (Kan<sup>r<\/sup>), if not specified individually<\/td>\n<\/tr>\n<tr>\n<td>Insert<\/td>\n<td>PCR amplification of <b><span style=\"text-decoration: underline;\">GENOMIC<\/span><\/b> DNA of the wild-type strain JY3 Amplified with 5&#8242;-G GGG ACA AGT TTG TAC AAA AAA GCA GGC TCT CAT ATG-(template specific sequence)-3&#8242; and 5&#8242;-GGG GAC CAC TTT GTA CAA GAA AGC TGG GTA-(template specific sequence)-3&#8242; as primers.<\/td>\n<\/tr>\n<tr>\n<td>Host<\/td>\n<td>DH5alpha, E. coli<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\">\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"4\">\n<tbody>\n<tr>\n<td colspan=\"2\">FLAG2-His6-tagged ORFs<\/td>\n<\/tr>\n<tr>\n<td>Vector<\/td>\n<td>pDUAL-FFH1c (Amp<sup>r<\/sup>), if not specified individually<\/td>\n<\/tr>\n<tr>\n<td>Insert<\/td>\n<td>Corresponding gene was cloned from the entry plasmid to pDUAL-FFH1c (RDB06142) using the Gateway technology.<\/td>\n<\/tr>\n<tr>\n<td>Host<\/td>\n<td>DH5alpha, E. coli<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\">\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"4\">\n<tbody>\n<tr>\n<td colspan=\"2\">YFH-tagged ORFs<\/td>\n<\/tr>\n<tr>\n<td>Vector<\/td>\n<td>pDUAL-YFH1c (Amp<sup>r<\/sup>), if not specified individually<\/td>\n<\/tr>\n<tr>\n<td>Insert<\/td>\n<td>Corresponding gene was cloned from the entry plasmid to pDUAL-YFH1c (RDB06151) using the Gateway technology.<\/td>\n<\/tr>\n<tr>\n<td>Host<\/td>\n<td>DH5alpha, E. coli<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\">1.2 Reference<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\"><a href=\"https:\/\/dnaconda.riken.jp\/DownLoad\/ORFeome_Localizome_data.csv\">ORFeome_Localizome_data.csv<\/a> (1.2 MB; 2007.07.02), provided by the depositor of S. pombe ORFeome plasmid.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Reference<\/h4>\n<ul>\n<li>Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Horaoka Y., Horinouchi S., Yoshida M. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast <i>Schizosaccharomyces pombe<\/i>. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/16823372\">Nature Biotech., 24, 841-847.(link)<\/a><\/li>\n<li>A dataset including the cloning status of the ORFeome entry clones, protein expression levels, and localizome data is available. Please obtain &#8220;Supplementary Table 6&#8221; of Nat. Biotechnol. 24 (7): 841-847 (2006).<\/li>\n<li>Bauer F, Matsuyama A, Candiracci J, Dieu M, Scheliga J, Wolf DA, Yoshida M, Hermand D. (2012) Translational control of cell division by elongator. Cell Rep. 1 (5): 424-433.<\/li>\n<li>Bauer F, Matsuyama A, Yoshida M, Hermand D. (2012) Determining proteome-wide expression levels using reverse protein arrays in fission yeast. Nat. Protoc. 7 (10): 1830-1835.<\/li>\n<li>Users&#8217; publication Informaion site<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05820\">Entry plasmid <img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/datasheet097.gif\" width=\"30\" \/><\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06106\"> FLAG2-His6-tagged ORF <img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/datasheet097.gif\" width=\"30\" \/><\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06107\">YFP-FLAG-His6-tagged ORF <img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/datasheet097.gif\" width=\"30\" \/><\/a>.<\/li>\n<\/ul>\n<h4>Related information<\/h4>\n<p>pDUAL vector series<br \/>\npDUAL2 vector series<br \/>\npREP vector series<br \/>\nPlease find <a href=\"https:\/\/brc.riken.jp\/dna\/GRP0027e\">vectors<\/a>.<br \/>\nPlasmid is shipped as DNA solution in TE buffer in an individual tube.<br \/>\nPlease specify ID of individual plasmid.<br \/>\n&nbsp;<br \/>\n<a href=\"#top\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/goup.gif\" border=\"0\" \/> go to Head<\/a><\/p>\n<hr \/>\n<p style=\"text-align: left;\">2025.05.03 (T.M. GRP0054e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Schizosaccharomyces pombe ORFeome plasmids were deposited by Dr. Minoru Yoshida, Chemical Genetics Lagoratory, RIKEN; These consists of 4,900 entry plasmids. Plasmids are provided individually. Establishing an entire protein assay system of &#8220;fission yeast&#8221;: Trends from genetic information to entire products in genomic analysis [2006.06.26 RIKEN Press release (pdf)] Localization data is available at S. pombe Postgenome Database The Schizosaccharomyces pombe Postgenome Database (SPD) contains the results of systematic analysis of protein expression and localization based on fission yeast ORFeome cloning as well as basic information on genes and proteins. The ORF collection currently covers 99.2% of the fission yeast protein-coding genes and pseudogenes that are reported by the Wellcome Trust Sanger Institute. Distribution of plasmids in Japanese 1. . Distribution of plasmids 1.1 Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must cite the following literature(s). (Matsuyama et al., 2006, Nature Biotech., 24, 841-847.). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must state an acknowledgement to the DEPOSITOR. When the USER wishes to sell the BIOLOGICAL RESOURCE and\/or its DERIVATIVES on commercial basis, the USER must obtain a prior written permission from [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8163,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8471","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8471","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8471"}],"version-history":[{"count":3,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8471\/revisions"}],"predecessor-version":[{"id":8476,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8471\/revisions\/8476"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8163"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8471"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}