{"id":8453,"date":"2025-05-03T08:20:58","date_gmt":"2025-05-02T23:20:58","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8453"},"modified":"2025-05-03T08:21:54","modified_gmt":"2025-05-02T23:21:54","slug":"carotenoiden","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/researchen\/carotenoiden","title":{"rendered":"Biosynthesis of carotenoid"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>Series of plasmids that confere biosynthesis of carotenoids in E. coli.<\/h2>\n<h3>List of plasmids in Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847.<\/h3>\n<hr width=\"50%\" \/>\n<h4>pACYC184 (Cm<sup>r<\/sup>)-based plasmid (without IDI)<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of resource<\/th>\n<th>Carotenoid biosynthesis genes<\/th>\n<th>Running title<\/th>\n<th>Reference<\/th>\n<\/tr>\n<tr>\n<td>RDB19483<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19483.html\">pACCRT-EB<\/a><\/td>\n<td>crtE, crtB<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of phytoene in E. coli (crtE, crtB).<\/td>\n<td>Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72259\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19484<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19484.html\">pACCRT-EIB<\/a><\/td>\n<td>crtE, crtB, crtI<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of lycopene in E. coli (crtE, crtB, crtI).<\/td>\n<td>Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72259\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19485<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19485.html\">pACCRT-EB-RcI<\/a><\/td>\n<td>crtE, crtB, Rc-crtI<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of neurosporene in E. coli (crtE, crtB, Rc-crtI).<\/td>\n<td>Takaichi, S. et al. 241 (1): 291-296 (1996). PMID 8898919. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72260\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19486<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19486.html\">pACCAR16 delta crtX<\/a><\/td>\n<td>crtE, crtB, crtI, crtY<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of \u03b2-carotene in E. coli (crtE, crtB, crtI, crtY).<\/td>\n<td>Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72259\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19487<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19487.html\">pACCAR25 delta crtX<\/a><\/td>\n<td>crtE, crtB, crtI, crtY, crtZ<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of zeaxanthin in E. coli (crtE, crtB, crtI, crtY, crtZ).<\/td>\n<td>Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72259\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19488<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19488.html\">pAC-Cantha<\/a><\/td>\n<td>crtE, crtB, crtI, crtY, crtW<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of canthaxanthin in E. coli (crtE, crtB, crtI, crtY, crtW).<\/td>\n<td>Nishida, Y. et al. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72261\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19489<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19489.html\">pAC-Asta<\/a><\/td>\n<td>crtE, crtB, crtI, crtY, crtZ, crtW<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of astaxanthin in E. coli (crtE, crtB, crtI, crtY, crtZ, crtW).<\/td>\n<td>Nishida, Y. et al. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72261\">[link to RRC of NBRP]<\/a><br \/>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<h4>pRK404 (Tc<sup>r<\/sup>)-based plasmid<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of resource<\/th>\n<th>Carotenoid biosynthesis genes<\/th>\n<th>Running title<\/th>\n<th>Reference<\/th>\n<\/tr>\n<tr>\n<td>RDB19490<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19490.html\">pRK-idi<\/a><\/td>\n<td>XdIDI<\/td>\n<td>Expression plasmid of the isopentenyl diphoshate (IPP) isomerase gene (IDI), which is used to incerease carotenoid production with another carotenogenic plasmid in E. coli (XdIDI).<\/td>\n<td>Albrecht, M. et al. Biotechnology Letters 21: 791?795 (1999). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72269\">[link to RRC of NBRP]<\/a><br \/>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<h4>pACYC184 (Cm<sup>r<\/sup>)-based plasmid (with IDI)<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of resource<\/th>\n<th>Carotenoid biosynthesis genes<\/th>\n<th>Running title<\/th>\n<th>Reference<\/th>\n<\/tr>\n<tr>\n<td>RDB19491<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19491.html\">pACHP-Phy<\/a><\/td>\n<td>HpIDI, crtE, crtB<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of phytoene (addtionally including the HpIDI gene) in E. coli. plasmid pACCRT-EB with HpIDI (HpIDI, crtE, crtB).<\/td>\n<td>Takemura, M. et al. BMC Zoology 6:19 (2021). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72268\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19492<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19492.html\">pACHP-Lyc<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of lycopene (addtionally including the HpIDI gene) in E. coli. Plasmid pACCRT-EIB with HpIDI (HpIDI, crtE, crtB, crtI).<\/td>\n<td>Takemura, M. et al. BMC Zoology 6:19 (2021). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72268\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19493<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19493.html\">pACHP-Beta<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of \u03b2-carotene (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR16\u0394crtX with HpIDI (HpIDI, crtE, crtB, crtI, crtY).<\/td>\n<td>Takemura, M. et al. Planta 241 (3): 699-710 (2015). PMID 25467956. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72262\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19494<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19494.html\">pACHP-Zea<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY, crtZ<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of zeaxanthin (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR25\u0394crtX with HpIDI (HpIDI, crtE, crtB, crtI, crtY, crtZ).<\/td>\n<td>Takemura, M.et al. Tetrahedron Letters 56(44): 6063-6065 (2015). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72267\">[link to RRC of NBRP]<\/a><br \/>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<h4>pACYC184 (Tc<sup>r<\/sup>)-based plasmid (with IDI)<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of resource<\/th>\n<th>Carotenoid biosynthesis genes<\/th>\n<th>Running title<\/th>\n<th>Reference<\/th>\n<\/tr>\n<tr>\n<td>RDB19495<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19495.html\">pAC-HIEB<\/a><\/td>\n<td>HpIDI, crtE, crtB<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of phytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19496<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19496.html\">pAC-HIEBI<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of lycopene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI).<\/td>\n<td>Takemura, M. et al. BMC Zoology 6:19 (2021). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72268\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19497<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19497.html\">pAC-HIEBIA<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, cruA<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of \u03b3-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, cruA).<\/td>\n<td>Takemura, M. et al. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72265\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19498<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19498.html\">pAC-HIEBIY<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of \u03b2-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19499<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19499.html\">pAC-HIEBIYZ<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY, crtZ<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of zeaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19500<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19500.html\">pAC-HIEBIYZW<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19501<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19501.html\">pAC-HIEBIYZG<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of nostoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19502<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19502.html\">pAC-HIEBI-ApCrtYB3<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, ApCrtYB3<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of \u03b2,\u03b3-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, ApCrtYB3).<\/td>\n<td>Takemura, M. et al. BMC Zoology 6:19 (2021). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72268\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19503<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19503.html\">pAC-HIEBI-ApCrtYB1<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, ApCrtYB1<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of torulene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, ApCrtYB1).<\/td>\n<td>Takemura, M. et al. BMC Zoology 6:19 (2021). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72268\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19504<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19504.html\">pAC-HIM<\/a><\/td>\n<td>HpIDI, crtM<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of 15-cis-4,4&#8242;-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM).<\/td>\n<td>Takemura, M. et al. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72264\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19505<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19505.html\">pAC-HIMN<\/a><\/td>\n<td>HpIDI, crtM, crtN<\/td>\n<td>pACYC184 vector-based plasmid for biosynthesis of 4,4&#8242;-diaponeurosporene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM, crtN).<\/td>\n<td>Takemura, M. et al. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72264\">[link to RRC of NBRP]<\/a><br \/>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<h4>pRK404 (Tc<sup>r<\/sup>)-based plasmid (with IDI)<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of resource<\/th>\n<th>Carotenoid biosynthesis genes<\/th>\n<th>Running title<\/th>\n<th>Reference<\/th>\n<\/tr>\n<tr>\n<td>RDB19506<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19506.html\">pRK-HIEB<\/a><\/td>\n<td>HpIDI, crtE, crtB<\/td>\n<td>pRK404 vector-based plasmid for biosynthesis of phytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB).<\/td>\n<td>Albrecht, M. et al. Biotechnology Letters 21: 791?795 (1999). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19507<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19507.html\">pRK-HIEBI<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI<\/td>\n<td>pRK404 vector-based plasmid for biosynthesis of lycopene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19508<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19508.html\">pRK-HIEBIY<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY<\/td>\n<td>pRK404 vector-based plasmid for biosynthesis of \u03b2-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19509<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19509.html\">pRK-HIEBIYZ<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY, crtZ<\/td>\n<td>pRK404 vector-based plasmid for biosynthesis of zeaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19510<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19510.html\">pRK-HIEBIYZW<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW<\/td>\n<td>pRK404 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19511<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19511.html\">pRK-HIEBIYZG<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG<\/td>\n<td>pRK404 vector-based plasmid for biosynthesis of nostoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19512<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19512.html\">pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, MpLCYb, MpLCYe, crtZ, Pag-crtE<\/td>\n<td>pRK404 vector-based plasmid for biosynthesis of zeinoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, MpLCYb, MpLCYe, crtZ, Pag-crtE).<\/td>\n<td>Takemura, M. et al. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72265\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19513<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19513.html\">pRK-HIEBIYZ-FhCCD2<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2<\/td>\n<td>pRK404 vector-based plasmid for biosynthesis of crocetindial (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19514<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19514.html\">pRK-HIEBIYZ-FhCCD2-NcALD8<\/a><\/td>\n<td>HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2, NcALD8<\/td>\n<td>pRK404 vector-based plasmid for biosynthesis of crocetin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2, NcALD8).<\/td>\n<td>Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72266\">[link to RRC of NBRP]<\/a><br \/>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<h4>Complementary plasmids for a series of carotenoid biosynthesis<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of resource<\/th>\n<th>Carotenoid biosynthesis genes<\/th>\n<th>Running title<\/th>\n<th>Reference<\/th>\n<\/tr>\n<tr>\n<td>RDB19515<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19515.html\">pUCBre-W<\/a><\/td>\n<td>Bre-crtW<\/td>\n<td>crtW-expression plasmid for production of astaxanthin and other ketocarotenoids when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli.<\/td>\n<td>Nishida, Y. et al. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72261\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19516<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19516.html\">pUCBre-O11<\/a><\/td>\n<td>crtG (Bre-crtG)<\/td>\n<td>crtG-expression plasmid for production of nostoxanthin and caloxanthin when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli.<\/td>\n<td>Nishida, Y. et al. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72261\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19517<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19517.html\">pUC-ApTor<\/a><\/td>\n<td>ApTor<\/td>\n<td>ApTOR-expression plasmid for production of 3,4-didehydrolycopene and lycopene when coexpressed with a plasmid to biosynthesize phytoene in E. coli.<\/td>\n<td>Takemura, M. et al. BMC Zoology 6:19 (2021). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72268\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19518<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19518.html\">pUC-CaZEP<\/a><\/td>\n<td>CaZEP<\/td>\n<td>CaZEP-expression plasmid for production of violaxanthin and antheraxanthin when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli.<\/td>\n<td>Takemura, M. et al. Appl. Microbiol. Biotechnol. 103 (23-24): 9393-9399 (2019). PMID 31673744. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72263\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19519<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19519.html\">pETDuet-MpLCYb\/MpLCYe <\/a><\/td>\n<td>MpLCYb, MpLCYe<\/td>\n<td>MpLCYb- and MpLCYe-expression plasmid for production of \u03b1-carotene (and \u03b2-carotene) when coexpressed with a plasmid to biosynthesize lycopene in E. coli.<\/td>\n<td>Takemura, M. et al. Planta 241 (3): 699-710 (2015). PMID 25467956. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72262\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19520<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19520.html\">pRSFDuet-MpBHY<\/a><\/td>\n<td>MpBHY<\/td>\n<td>MpBHY-expression plasmid for production of zeinoxanthin (and zeaxanthin) when coexpressed with a plasmid to biosynthesize \u03b1-carotene (and \u03b2-carotene) in E. coli.<\/td>\n<td>Takemura, M. et al. Planta 241 (3): 699-710 (2015). PMID 25467956. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72262\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19521<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19521.html\">pRSFDuet-MpBHY\/MpCYP97C<\/a><\/td>\n<td>MpBHY, MpCYP97C<\/td>\n<td>MpBHY- and MpCYP97C-expression plasmid for production of lutein (and zeaxanthin) when coexpressed with a plasmid to biosynthesize \u03b1-carotene (and \u03b2-carotene) in E. coli.<\/td>\n<td>Takemura, M. et al. Planta 241 (3): 699-710 (2015). PMID 25467956. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72262\">[link to RRC of NBRP]<\/a><\/p>\n<tr>\n<td>RDB19522<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19522.html\">CDF-MpCYP97C-MpLCYe<\/a><\/td>\n<td>MpCYP97C, MpLCYe<\/td>\n<td>MpCYP97C- and MpLCYe-expression plasmid for production of lutein when coexpreessd with a plasmid to biosynthsize zeinoxanthin in E. coli.<\/td>\n<td>Takemura, M. et al. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=72265\">[link to RRC of NBRP]<\/a><br \/>\n<\/tbody>\n<\/table>\n<p>Please refer Fig. 1 of Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022) for the structure of plasmids.<\/p>\n<hr \/>\n<p style=\"text-align:left;\">2025.05.03 (T.M. GRP0075e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Series of plasmids that confere biosynthesis of carotenoids in E. coli. List of plasmids in Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. pACYC184 (Cmr)-based plasmid (without IDI) Catalog # Name of resource Carotenoid biosynthesis genes Running title Reference RDB19483 pACCRT-EB crtE, crtB pACYC184 vector-based plasmid for biosynthesis of phytoene in E. coli (crtE, crtB). Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP] RDB19484 pACCRT-EIB crtE, crtB, crtI pACYC184 vector-based plasmid for biosynthesis of lycopene in E. coli (crtE, crtB, crtI). Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP] RDB19485 pACCRT-EB-RcI crtE, crtB, Rc-crtI pACYC184 vector-based plasmid for biosynthesis of neurosporene in E. coli (crtE, crtB, Rc-crtI). Takaichi, S. et al. 241 (1): 291-296 (1996). PMID 8898919. [link to RRC of NBRP] RDB19486 pACCAR16 delta crtX crtE, crtB, crtI, crtY pACYC184 vector-based plasmid for biosynthesis of \u03b2-carotene in E. coli (crtE, crtB, crtI, crtY). Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP] RDB19487 pACCAR25 delta crtX crtE, crtB, crtI, crtY, crtZ pACYC184 vector-based plasmid for biosynthesis of zeaxanthin in E. [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8163,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8453","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8453","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8453"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8453\/revisions"}],"predecessor-version":[{"id":8457,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8453\/revisions\/8457"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8163"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8453"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}