{"id":6182,"date":"2017-10-03T18:37:14","date_gmt":"2017-10-03T09:37:14","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=6182"},"modified":"2021-05-27T08:26:33","modified_gmt":"2021-05-26T23:26:33","slug":"rvd_sopen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/manualen\/rvd_sopen","title":{"rendered":"Recombinant virus protocols"},"content":{"rendered":"<p><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0002j\">in Japanese<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/jpn.gif\" alt=\"\" border=\"0\" \/><\/a><\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"6\" bgcolor=\"#FFFFD0\">\n<tbody>\n<tr>\n<th colspan=\"4\" align=\"Left\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/vicon02.gif\" width=\"13\" height=\"13\" border=\"0\" \/><span style=\"font-size: small;\">Adenovirus vector<\/span><\/th>\n<\/tr>\n<tr bgcolor=\"#D0D0FF\">\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Title<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Language<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Format<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">PDF<\/span><\/th>\n<\/tr>\n<tr>\n<td width=\"620\">SOP for High-sensitivity Detection Recombinant Adenovirus DNA by PCR<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Adv\/SOP-AdV-002EM.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Adv\/SOP-AdV-002E.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"620\">Purification of recombinant adenoviruses<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Adv\/Adv-003en.html\">HTML<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Adv\/Adv-003en.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a name=\"Ade-ass\"><\/a><\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"6\" bgcolor=\"#FFFFD0\">\n<tbody>\n<tr>\n<th colspan=\"4\" align=\"Left\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/vicon02.gif\" width=\"13\" height=\"13\" border=\"0\" \/><span style=\"font-size: small;\">Adeno-associated Virus vector<\/span><\/th>\n<\/tr>\n<tr bgcolor=\"#D0D0FF\">\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Title<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Language<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Format<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">PDF<\/span><\/th>\n<\/tr>\n<tr>\n<td width=\"620\">SOP for High-sensitivity Detection of Adeno-Associated Virus(AAV)Vecter by PCR<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/SOP-AAV-001EM.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/SOP-AAV-001E.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"620\">SOP for Preparation of the AAV Vector Standard<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/SOP-AAV-002EM.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/SOP-AAV-002E.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"620\">SOP for Measurement of the Copy Number of the AAV Vector in Genomic DNA Using the AAV Vector Standard<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/SOP-AAV-003EM.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/SOP-AAV-003E.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"620\">A Protocol for production and purification of AAV vector<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/AAVProtocol.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/AAVProtocol.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"620\">A protocol for enhancement of the AAV-mediated expression of transgenes<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/enhancement.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/enhancement.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"620\">Construction of pAAneo adeno-associated virus vector<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/pAAneo.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/AAV\/pAAneo.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a name=\"Retro\"><\/a><\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"6\" bgcolor=\"#FFFFD0\">\n<tbody>\n<tr>\n<th colspan=\"4\" align=\"Left\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/vicon02.gif\" width=\"13\" height=\"13\" border=\"0\" \/><span style=\"font-size: small;\">Retrovirus vector<\/span><\/th>\n<\/tr>\n<tr bgcolor=\"#D0D0FF\">\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Title<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Language<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Format<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">PDF<\/span><\/th>\n<\/tr>\n<tr>\n<td width=\"620\">SOP for Detection by PCR of a Recombinant Gene in Cells Infected with the MFG Retrovirus Vector (psi-CRIP series)<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Retro\/SOP-MFG-002EM.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Retro\/SOP-MFG-002E.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"620\">Construction of retroviral vectors, pMFGneo and pRx-nZiresNeo<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Retro\/pMFGneo.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Retro\/pMFGneo.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a name=\"Hepatitis\"><\/a><\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"6\" bgcolor=\"#FFFFD0\">\n<tbody>\n<tr>\n<th colspan=\"4\" align=\"Left\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/vicon02.gif\" width=\"13\" height=\"13\" border=\"0\" \/><span style=\"font-size: small;\">Hepatitis C virus vector<\/span><\/th>\n<\/tr>\n<tr bgcolor=\"#D0D0FF\">\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Title<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Language<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Format<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">PDF<\/span><\/th>\n<\/tr>\n<tr>\n<td width=\"620\">SOP for Quantitation of the Hapatitis C Virus(HCV)Genome by RT-PCR<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/HCV\/SOP-HCV-001EM.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/HCV\/SOP-HCV-001E.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a name=\"Others\"><\/a><\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"6\" bgcolor=\"#FFFFD0\">\n<tbody>\n<tr>\n<th colspan=\"4\" align=\"Left\"><span style=\"font-size: small;\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/vicon02.gif\" width=\"13\" height=\"13\" border=\"0\" \/>Others<\/span><\/th>\n<\/tr>\n<tr bgcolor=\"#D0D0FF\">\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Title<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Language<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">Format<\/span><\/th>\n<th nowrap=\"nowrap\"><span style=\"color: purple;\">PDF<\/span><\/th>\n<\/tr>\n<tr>\n<td width=\"620\">SOP for Extraction of Genomic DNA from Cultured Cells Using the DNAzol<sup>(R)<\/sup>Genomic DNA-Isolation Reagent<\/td>\n<td align=\"center\" nowrap=\"nowrap\">English<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Others\/SOP-MFG-001EM.doc\">MS-Word<\/a><\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Others\/SOP-MFG-001E.pdf\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/pdficon.gif\" alt=\"PDF\" border=\"0\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<p style=\"text-align: right;\">(MAN0002e 2010.05.19 T.M.)<\/p>\n<p style=\"text-align: left;\">2021.05.27<\/p>\n","protected":false},"excerpt":{"rendered":"<p>in Japanese Adenovirus vector Title Language Format PDF SOP for High-sensitivity Detection Recombinant Adenovirus DNA by PCR English MS-Word Purification of recombinant adenoviruses English HTML Adeno-associated Virus vector Title Language Format PDF SOP for High-sensitivity Detection of Adeno-Associated Virus(AAV)Vecter by PCR English MS-Word SOP for Preparation of the AAV Vector Standard English MS-Word SOP for Measurement of the Copy Number of the AAV Vector in Genomic DNA Using the AAV Vector Standard English MS-Word A Protocol for production and purification of AAV vector English MS-Word A protocol for enhancement of the AAV-mediated expression of transgenes English MS-Word Construction of pAAneo adeno-associated virus vector English MS-Word Retrovirus vector Title Language Format PDF SOP for Detection by PCR of a Recombinant Gene in Cells Infected with the MFG Retrovirus Vector (psi-CRIP series) English MS-Word Construction of retroviral vectors, pMFGneo and pRx-nZiresNeo English MS-Word Hepatitis C virus vector Title Language Format PDF SOP for Quantitation of the Hapatitis C Virus(HCV)Genome by RT-PCR English MS-Word Others Title Language Format PDF SOP for Extraction of Genomic DNA from Cultured Cells Using the DNAzol(R)Genomic DNA-Isolation Reagent English MS-Word (MAN0002e 2010.05.19 T.M.) 2021.05.27<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":3960,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6182","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/6182","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=6182"}],"version-history":[{"count":5,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/6182\/revisions"}],"predecessor-version":[{"id":7494,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/6182\/revisions\/7494"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3960"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=6182"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}