*********************************************************
\nRIKEN BioResource Center DNA Bank Mail News
\n*********************************************************<\/p>\n
:: CONTENT ::::::::::::::::::::::::::::::::
\n– B6N Mouse BAC clone is now available
\n– Addition of data of immunofluorescent staining for the full CDS expression clones
\n:::::::::::::::::::::::::::::::::::::::::::::<\/p>\n
=======================================================
\n– B6N Mouse BAC clone is now available
\n=======================================================
\nThe Gene Engineering Division has constructed a BAC library of the B6N substrain and the RIKEN BioResource Center completed BAC end sequencing of B6N and register the sequence data with DDBJ in a collaboration with the National Institute of Genetics under the Genome Information Upgrading Program of MEXT NBRP. The B6N Mouse BAC library consisted of 62,000 clones representing an estimated coverage of 2.6-fold haploid genome with 90% provability.<\/p>\n
A new database, ‘Mouse BAC Brower’ is also available at the site of the National Institute of Genetics . This database provides information of
\ngenomic locations of the B6N Mouse BAC clones on a B6J’s genome sequence as well as the context of other features, such as genes.<\/p>\n
Using the database, researchers can be find BAC clones in silico with direct querying (ex. gene symbol, name of gene product, gene ID and so on).<\/p>\n
If you are interested in our BAC clones, please feel free to contact us.<\/p>\n
The germ cell competent C57BL\/6N ES cell lines are available from the Cell Engineering Division of RIKEN BRC. <\/p>\n
=======================================================
\n– Addition of immunofluorescent staining data for the full CDS expression clones
\n=======================================================
\nThe Gene Engineering Division provides full CDS expression clones that were constructed by our division. Their entire nucleotide sequences were confirmed. Furthermore, their expression proteins were also confirmed by the western blotting and the immunofluorescent staining. <\/p>\n
We opened additional data of immunofluorescent staining for cells introduced expression vectors listed below.<\/p>\n
If you are interested in our expression clones, please feel free to contact us.<\/p>\n
List of genes
\nRDB 6068 human PPARD\/PPAR delta
\nRDB 6069 human ROR beta
\nRDB 6070 human TR beta; ErbA-beta
\nRDB 6108 human CREB3
\nRDB 6111 human NFE2
\nRDB 6295 human ER beta
\nRDB 6598 human Oct3\/4 isoform1
\nRDB 6600 human Sox2
\nRDB 6601 human Nanog homeobox
\nRDB 6602 human LIN28
\nRDB 6670 human Klf4
\nRDB 6671 human c-Myc
\nRDB 6998 human IL6
\nRDB 6999 human CPSF5\/CPSF25
\nRDB 7012 human CREB2; ATF4
\nRDB 7013 human NRF1; NFE2L1
\nRDB 7014 human NRF1; NFE2L1
\nRDB 7015 human NRF3; NFE2L3
\nRDB 7016 human E2F8
\nRDB 7017 human NFKB2
\nRDB 7018 human NFKB1
\nRDB 7019 human SMAD3
\nRDB 7020 human Sap-1
\nRDB 7026 human MYCL1, transcript variant 3
\nRDB 7027 human neural cell adhesion molecule 1, transcript variant 1
\nRDB 7028 human AKT2
\nRDB 7029 human v-myc myelocytomatosis viral oncogene homolog (avian)
\nRDB 7270 human PEA3
\nRDB 7271 human SRY(sex determining region Y)-box 11<\/p>\n
2010.03.01<\/p>\n","protected":false},"excerpt":{"rendered":"
********************************************************* RIKEN BioResource Center DNA Bank Mail News ********************************************************* :: CONTENT :::::::::::::::::::::::::::::::: – B6N Mouse BAC clone is now available – Addition of data of immunofluorescent staining for the full CDS expression clones ::::::::::::::::::::::::::::::::::::::::::::: ======================================================= – B6N Mouse BAC clone is now available ======================================================= The Gene Engineering Division has constructed a BAC library of the B6N substrain and the RIKEN BioResource Center completed BAC end sequencing of B6N and register the sequence data with DDBJ in a collaboration with the National Institute of Genetics under the Genome Information Upgrading Program of MEXT NBRP. The B6N Mouse BAC library consisted of 62,000 clones representing an estimated coverage of 2.6-fold haploid genome with 90% provability. A new database, ‘Mouse BAC Brower’ is also available at the site of the National Institute of Genetics . This database provides information of genomic locations of the B6N Mouse BAC clones on a B6J’s genome sequence as well as the context of other features, such as genes. Using the database, researchers can be find BAC clones in silico with direct querying (ex. gene symbol, name of gene product, gene ID and so on). If you are interested in our BAC clones, please feel free to contact […]<\/p>\n","protected":false},"author":9,"featured_media":0,"parent":7019,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-5009","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/5009","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=5009"}],"version-history":[{"count":17,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/5009\/revisions"}],"predecessor-version":[{"id":6866,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/5009\/revisions\/6866"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/7019"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=5009"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}