{"id":3911,"date":"2015-01-14T14:23:08","date_gmt":"2015-01-14T05:23:08","guid":{"rendered":"http:\/\/dna1.dna.rbrc.jp\/en\/?page_id=3911"},"modified":"2024-04-08T16:56:27","modified_gmt":"2024-04-08T07:56:27","slug":"furnish60","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/orderingen\/furnish60","title":{"rendered":"Quality control"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>Quality control<\/h2>\n<p>We distribute<br \/>\n(1) plasmid and cosmid clones,<br \/>\n(2) BAC, YAC, P1, fosmid, cosmid from genomic clone libraries,<br \/>\n(3) recombinant adenoviruses,<br \/>\n(4) bacterial host,<br \/>\n(5) cDNA library,<br \/>\n(6) DNA clone set,<br \/>\n(7) microbial genome DNA, and<br \/>\n(8) mouse genome DNA.<br \/>\nEach product is tested in order to ensure the reproducibility of experimental results.<\/p>\n<h3>1.<span style=\"text-decoration: underline;\"> Plasmid and cosmid clones<\/span><\/h3>\n<p style=\"padding-left: 30px;\">Plasmids and cosmids are provided as DNA solution in TE buffer at ambient temperature. Plasmids and cosmids are amplified in transformed bacteria.<br \/>\nInformation on nucleotide sequence, protein production and other properties obtained by the depositor\/developer are shown in the web catalog.<\/p>\n<p style=\"padding-left: 30px;\"><span style=\"text-decoration: underline;\">Quality Test:<\/span> Based on resource information provided by the depositor\/developer, we perform the following tests:<\/p>\n<ul>\n<li><span style=\"text-decoration: underline;\">Performed:<\/span>\n<ul>\n<li>Growth and antibiotics resistance of the recombinant bacteria in the culture conditions specified by the depositor\/developer<\/li>\n<li>Restriction enzyme mapping<\/li>\n<li>High Throughput Sequencing and\/or Sanger sequencing around vector-insert junction and important portion(s) of vector<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Not Performed:<\/span>\n<ul>\n<li>Protein production<\/li>\n<li>Promoter activity, etc.<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Supplemental Tests:<\/span>\n<ul>\n<li>When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3>2.<span style=\"text-decoration: underline;\">BAC, YAC, P1, fosmid, cosmid from genomic clone libraries<\/span><\/h3>\n<p style=\"padding-left: 30px;\">BAC, YAC, P1, fosmids, cosmids from genomic clone libraries are provided as recombinant bacteria.<br \/>\nInformation on nucleotide sequence and other properties obtained by the depositor\/developer are shown in the web catalog.<\/p>\n<p style=\"padding-left: 30px;\"><span style=\"text-decoration: underline;\">Quality Test:<\/span> Based on resource information provided by the depositor\/developer, we perform the following tests:<\/p>\n<ul>\n<li><span style=\"text-decoration: underline;\">Performed:<\/span>\n<ul>\n<li>Growth and antibiotics resistance of the recombinant bacteria in the culture conditions specified by the depositor\/developer<\/li>\n<li>PCR to detect endo sequence registered in the DNA sequence database<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li><span style=\"text-decoration: underline;\">Not Performed:<\/span>\n<ul>\n<li>Restriction enzyme mapping<\/li>\n<li>Full length or terminal nucleotide sequencing, etc.<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Supplemental Tests:<\/span>\n<ul>\n<li>When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3>3.<span style=\"text-decoration: underline;\"> Recombinant adenoviruses<\/span><\/h3>\n<p style=\"padding-left: 30px;\">Recombinant adenovirus is amplified by infection to HEK293 cells. An aliquot of crude lysates of infected cells is provided.<br \/>\nInformation on nucleotide sequence and other properties obtained by the depositor\/developer are shown in the web catalog.<\/p>\n<p style=\"padding-left: 30px;\"><span style=\"text-decoration: underline;\">Quality Test:<\/span> Based on resource information provided by the depositor\/developer, we perform the following tests:<\/p>\n<ul>\n<li><span style=\"text-decoration: underline;\">Performed:<\/span>\n<ul>\n<li>Restriction enzyme mapping<\/li>\n<li>PCR amplification of E1 region to confirm that replication competent adenovirus (RCA) is under the detection level<\/li>\n<li>Nucleotide sequencing of vector-insert junction area and portions of vector sequences<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Not Performed:<\/span>\n<ul>\n<li>Full length nucleotide sequencing of virus<\/li>\n<li>Protein production, etc.<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Supplemental Tests:<\/span>\n<ul>\n<li>When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>4.<span style=\"text-decoration: underline;\">Bacterial host<\/span><\/h3>\n<p style=\"padding-left: 30px;\">Bacterial host is shipped as a stab-culture in a plastic vial at ambient temperature. Information on nutrient requirement and other properties obtained by the depositor\/developer are shown in the web catalog.<\/p>\n<p style=\"padding-left: 30px;\"><span style=\"text-decoration: underline;\">Quality Test:<\/span> Based on resource information provided by the depositor\/developer, we perform the following tests:<\/p>\n<ul>\n<li><span style=\"text-decoration: underline;\">Performed:<\/span>\n<ul>\n<li>Nutrient requiring, growth and antibiotics resistance of the host bacteria in the culture conditions specified by the depositor\/developer<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Not Performed:<\/span>\n<ul>\n<li>Genotyping, etc.<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Supplemental Tests:<\/span>\n<ul>\n<li>When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>5.<span style=\"text-decoration: underline;\"> cDNA Library<\/span><\/h3>\n<p style=\"padding-left: 30px;\">cDNA libraries are amplified once. Information on number of independent clones, range and average of the insert size and other properties obtained by the depositor\/developer are shown in the web catalog.<\/p>\n<p style=\"padding-left: 30px;\"><span style=\"text-decoration: underline;\">Quality Test:<\/span> Based on resource information provided by the depositor\/developer, we perform the following tests:<\/p>\n<ul>\n<li><span style=\"text-decoration: underline;\">Performed:<\/span>\n<ul>\n<li>Growth and antibiotics resistance of the recombinant bacteria in the culture conditions specified by the depositor\/developer<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Not Performed:<\/span>\n<ul>\n<li>Number of independent clones<\/li>\n<li>Range and average of the insert size, etc.<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Supplemental Tests:<\/span>\n<ul>\n<li>When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3>6.<span style=\"text-decoration: underline;\">DNA clone set<\/span><\/h3>\n<p style=\"padding-left: 30px;\">Master replica plate (the first copy from the originally deposited plate) is copied from the original set. Provided set is made from the master replica plates as a copy. Information on nucleotide sequence, protein production and other properties obtained by the depositor\/developer are shown in the web catalog.<\/p>\n<p style=\"padding-left: 30px;\"><span style=\"text-decoration: underline;\">Quality Test:<\/span> Based on resource information provided by the depositor\/developer, we perform the following tests:<\/p>\n<ul>\n<li><span style=\"text-decoration: underline;\">Performed:<\/span>\n<ul>\n<li>Growth and antibiotics resistance of the recombinant bacteria in the culture conditions specified by the depositor\/developer<\/li>\n<li>Restriction enzyme mapping of randomly picked up clones<\/li>\n<li>Nucleotide sequencing around vector-insert junction area of randomly picked up clones<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Not Performed:<\/span>\n<ul>\n<li>Restriction mapping of individual clones of entire set<\/li>\n<li>Full length or terminal nucleotide sequences of individual clones of the entire set<\/li>\n<li>Protein production, etc.<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Supplemental Tests:<\/span>\n<ul>\n<li>When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3>7.<span style=\"text-decoration: underline;\"> Microbial genome DNA<\/span><\/h3>\n<p style=\"padding-left: 30px;\">Microorganisms are cultured by the Microbe Division (JCM). Genomic DNA is extracted by the phenol-chloroform extraction by the Gene Engineering Division. Information on nucleotide sequence and other properties obtained by the depositor\/developer are shown in the web catalog.<\/p>\n<p style=\"padding-left: 30px;\"><span style=\"text-decoration: underline;\">Quality Test:<\/span> Based on resource information provided by the depositor\/developer, we perform the following tests:<\/p>\n<ul>\n<li><span style=\"text-decoration: underline;\">Performed:<\/span>\n<ul>\n<li>Concentration (A260) and purity (A260\/A280) of genomic DNA measured by a spectrophotometer<\/li>\n<li>Agarose gel electrophoresis to determine concentration (comparing to standard sample) and DNA fragmentation<\/li>\n<li>PCR amplification of 16S rRNA gene<\/li>\n<li>Nucleotide sequence of 16S rRNA gene<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Not Performed:<\/span>\n<ul>\n<li>Genetic back ground of strain by genetic marker, etc.<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Supplemental Tests:<\/span>\n<ul>\n<li>When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3>8.<span style=\"text-decoration: underline;\">Mouse genome DNA<\/span><\/h3>\n<p style=\"padding-left: 30px;\">Mice for DNA extraction are maintained by the Experimental Animal Division. Genomic DNA is extracted by the phenol-chloroform extraction by the Gene Engineering Division. Information on any other properties obtained by the depositor\/developer are shown in the web catalog.<\/p>\n<p style=\"padding-left: 30px;\"><span style=\"text-decoration: underline;\">Quality Test:<\/span> Based on resource information provided by the depositor\/developer, we perform the following tests:<\/p>\n<ul>\n<li><span style=\"text-decoration: underline;\">Performed:<\/span>\n<ul>\n<li>Concentration (A260) and purity (A260\/A280) of genomic DNA measured by a spectrophotometer<\/li>\n<li>Agarose gel electrophoresis to determine concentration (comparing to standard sample) and DNA fragmentation<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Not Performed:<\/span>\n<ul>\n<li>Genetic back ground of a strain by genetic markers, etc.<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Supplemental Tests:<\/span>\n<ul>\n<li>When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p style=\"text-align:right;\">(2014.09.22 T.M.)<\/p>\n<p style=\"text-align:left;\">2024.04.08<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Quality control We distribute (1) plasmid and cosmid clones, (2) BAC, YAC, P1, fosmid, cosmid from genomic clone libraries, (3) recombinant adenoviruses, (4) bacterial host, (5) cDNA library, (6) DNA clone set, (7) microbial genome DNA, and (8) mouse genome DNA. Each product is tested in order to ensure the reproducibility of experimental results. 1. Plasmid and cosmid clones Plasmids and cosmids are provided as DNA solution in TE buffer at ambient temperature. Plasmids and cosmids are amplified in transformed bacteria. Information on nucleotide sequence, protein production and other properties obtained by the depositor\/developer are shown in the web catalog. Quality Test: Based on resource information provided by the depositor\/developer, we perform the following tests: Performed: Growth and antibiotics resistance of the recombinant bacteria in the culture conditions specified by the depositor\/developer Restriction enzyme mapping High Throughput Sequencing and\/or Sanger sequencing around vector-insert junction and important portion(s) of vector Not Performed: Protein production Promoter activity, etc. Supplemental Tests: When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog. 2.BAC, YAC, P1, fosmid, cosmid from genomic clone libraries BAC, YAC, P1, fosmids, cosmids from genomic clone libraries are provided as recombinant [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":3901,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-3911","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3911","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=3911"}],"version-history":[{"count":9,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3911\/revisions"}],"predecessor-version":[{"id":8184,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3911\/revisions\/8184"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3901"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=3911"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}