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Thermus thermophilus Expression plasmid


Thermus thermophilus HB8 expression plasmids were constructed by “Whole-Cell Project of a Model Organism, Thermus thermophilus HB8” (http://www.thermus.org/) and deposited by Dr. Seiki Kuramitsu.

How to obtain Expression clones


This page

Plasmid clones for producing recombinant protein of Thermus thermophilus HB8 gene products in E. coli.
Clone(s) is shipped as DNA solution in TE buffer in individual tube.

Outside database

Please find ID of individual clone(s) using the following outside database
http://www.tanpaku.org/ThermusDB/index.html
or this summary table.

Forms

Please specify ID (ex. TEx01A01 or PC010526-41) of individual clone(s).

Please indicate Terms and Conditions for Distribution in MTA as follows:

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER is expected to cite the following literature.
(Yokoyama et al., 2000, Nature Struct. Biol. 7, 943-945).

Please complete forms:

Order Form, FormA (MS-Word)
Material Transfer Agreement, FormC (pdf)
Order Form (FormA) [Open a new window!!]
Please complete the form with your shipping information including your account number of an international courier (FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
NOTE: User registration on our system is required before download the Order Form for credit card (Visa / Master).
Material Transfer Agreement (Category I MTA) [Word]
For the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization.

We ask a signature of the Authorized Representative of a recipient institution.
In the Section 2(a), please write your purpose of use of clone in 10 to 20 words.

(Please e-mail to dnabank.brc@riken.jp to obtain MTA in MS-Word format)
Material Transfer Agreement (Category II MTA) [Word]
For the use of our bioresource in research for the following cases:
 1. For research to be conducted by for-profit organizations.
 2. For collaborative research between for-profit organization and not-for-profit organization.
 3, For research by not-for- organization outsourced and sponsored by for-profit organization.
 4. For for-profit research by not-for-profit organization including R&D with the aim of patent acquisition.

Send forms:

The DNA Bank, RIKEN BioResource Research Center,
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
E-mail: dnabank.brc@riken.jp
FAX: (+81)-29-836-9120

Please visit further information of distribution and fees.

Distribution fee per clone:
8,640 YEN (For use in research for not-for-profit academic purpose).
17,280 YEN (For use in research for-profit-research purpose).
(Shipping cost is not included.)

For details for fee and payment, please visit Information of Request for Distribution.

Personal data protection policy

For protection of personal data at RIKEN BioResource Research Center, please reffer Personal data protection policy.

Data Sheet

1. Thermus thermophilus HB8 expression plasmid
1.1 Strain information
Species Thermus thermophilus
Strain Thermus thermophilus HB8
1.2 Clone information
Vector pET-11a (Ampr), pET-11b (Ampr) or pET-3a (Ampr)
Insert genomic DNA
Host Plasmid was amplified using DH5alpha, E. coli
Clone data is available at the “Download” page at? https://dna.brc.riken.jp/en/thermus_4en.html
1.3 Reference
1 Yokoyama, S., Hirota, H., Kigawa, T., Yabuki, T., Shirouzu, M., Terada, T., Ito, Y., Matsuno, Y. Kuroda, Y., Nishimura, Y., Kyogoku, Y., Miki, K., Masui, R., Kuramitsu, S. (2000)? Structual genomics projects in Japan. Nature Struct. Biol. 7, 943-945.
2 http://www.thermus.org/
3 Summary of constructed clones https://www.brc.riken.jp/lab/dna/en/thermus_4en.html
4 Whole Cell Project Database http://www.srg.harima.riken.jp/h_db/index.html

List of publication using this resource

Catalog No. Title Journal Author PMID
(7723) T. thermophilus HB8 Disruption Plasmid TTHA1619 N7-Methylguanine at position 46 (m7G46) in tRNA from Thermus thermophilus is required for cell viability at high temperatures through a tRNA modification network. Nucleic Acids Res. 2009 Nov 24. [Epub ahead of print] (2009). Tomikawa, C., Yokogawa, T., Kanai, T., Hori, H. 19934251
(6078) Thermus thermophilus expression plasmids

TTHA0132? TTHA0271 TTHA0281? TTHA0341

TTHA1053? TTHA1091 TTHA1199? TTHA1275

TTHA1359? TTHA1671 TTHA1699? TTHA1713

TTHA1838? TTHA1897 TTHB192

Thermus thermophilus-derived protein tags that aid in preparation of insoluble viral proteins. Anal. Biochem. 385 (2): 278-285 (2009). Kondo, N., Ebihara, A., Ru, H., Kuramitsu, S., Iwamoto, A., Rao, Z., Matsuda, Z. 19084492

List of Related Articles of This Resource

Title Journal Author PMID
Molecular mechanisms of the whole DNA repair system: a comparison of bacterial and eukaryotic systems. J. Nucleic Acids 2010:179594 (2010). Morita, R., Nakane, S., Shimada, A., Inoue, M., Iino, H., Wakamatsu, T., Fukui, K., Nakagawa, N., Masui, R., Kuramitsu, S. 20981145
Discovery and Analysis of Cofactor-dependent Phosphoglycerate Mutase Homologs as Novel Phosphoserine Phosphatases in Hydrogenobacter thermophilus. J Biol Chem. 287 (15): 11934-11941 (2012). Chiba, Y., Oshima, K., Arai, H., Ishii, M., Igarashi, Y. 22337887

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(GRP0051e 2012.10.22 T.M.)

2018.10.05