Gene Engineering Division

General Information

 
Genomic DNA is prepared in the Gene Engineering Division from mouse strains maintained in the RIKEN BRC Experimental Animal Division. High molecular weight DNA is prepared by phenol-chloroform extraction. The DNA is suitable for amplification by PCR. The concentration and purity of DNA samples are determined spectrophotometrically following electrophoresis. DNA concentrations generally range from 50 to 100 ng/μl. (The concentrations of individual samples are supplied with a data sheet.) The DNAs are stored in TE buffer (10 mM TRIS pH7.5, 1mM EDTA) at -30^oC.

Attention International Transfer – “Veterinary Permission” or “Permit to Import Quarantine Material” may be required for shipment of the biological materials. Please follow guide lines in your country.

Forms for Distribution

Forms required

Forms	Description
Form A	Order Form Please specify strain names with RDB numbers (catalog number).Please complete the form with your shipping information including your account number of an international courier (FedEx. World Courier, TNT Express, DHL Global Forwarding and others). List of DNA
Form C	Material Transfer Agreement We would like to ask a signature of the Authorized Representative of a recipient institution.In the the Section 2(a), please write your purpose of use of clone in 10 to 20 words.Please put the terms and condisions that is indicated in the individual data sheet of the genomic DNA.

Order Form: Please complete the form with your shipping information including your account number of an international courier (FedEx. World Courier, TNT Express, DHL Global Forwarding and others). See detail in Information of Request for Distribution Note: The resident of the European Economic Area (EEA) and China, please read Special distribution Information to Residents of the Foreign Countries

Order Fom for Credit Card Payment. (Visa or Master Card only) [Word]

Order Form for Bank Transfer Payment. [Word]

Material Transfer Agreement (Category I MTA) [Word] For the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization. Regarding Section 2(a): Please write your research purpose in detail. We need description specifically how and for what purpose you are going to use the DNA Bank resource(s). If the information is considered insufficient, we may ask you to add more or rewrite it. We can check whether the documents are filled out correctly or not in advance. Please email documents to us. Regarding signature line: "Authorized Representative" is a person who is responsible for intellectual property rights. We request that the candidate is in one of the following positions, he/she can sign the MTA as the Authorized Representative. For anything unclear, please contact us by email. College/University/Graduate School: President, Dean, Director or Head of Department Research Institution: Director Corporation: President, CEO, Director Any officer appointed as Intellectual Property Administrator by the organization The MTA is a formal contract to be executed between institutions. Therefore, we ask your Authorized Representative to be an authority listed above.

Material Transfer Agreement (Category II MTA) [Word] For the use of our bioresource in research for the following cases: For research to be conducted by for-profit organizations. For collaborative research between for-profit organization and not-for-profit organization. For research by not-for- organization outsourced and sponsored by for-profit organization. For for-profit research by not-for-profit organization including R&D with the aim of patent acquisition.

Please visit Information of Request for Distribution and Fee and Payment for further information.

Related Information

Reconstitution of DNA

• In the case you received plastic vials contains precipitated DNA with ethanol.
• A precipitant might stick to the lid or wall during transportation.
• Before opening the tube, please spin down a minute or two at 5,000 g (ca. 7,000 rpm, table top microfuge).
• Please remove ethanol with pipet (DO NOT tooch precipitate!)
• Immediately after removal of ethanol resuspend the DNA in an appropriate amount of TE buffer (10 mM Tris-Cl, pH 7.5, 1 mM EDTA), and dissolve the DNA pellet by gently tapping with fingers (DO NOT shake vigorously! A little amount of ethanol won’t affect to many applications).
• If the DNA is difficult to solve, leave the vial at 4 degreeC overnight to allow the DNA to dissolve.
• Once reconstituted in TE buffer, the DNA should be stored at -30 degreeC.

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(2014.06.18 T.M.)

2019.02.17