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Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

BRC News

[RIKEN BRC] DNA Bank Mail News 2015.03.18.en


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RIKEN BioResource Center DNA Bank Mail News
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:: CONTENT ::::::::::::::::::::::::::::::::
– The 2015 Annual Conference of the Japan Society for Bioscience,
Biotechnology and Agrochemistry
– Pick Up Resource – AID system –
– Focused Resource – Reference of materials –
– Link between KEGG and our genetic materials by the LinkDB
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– The 2015 Annual Conference of the Japan Society for Bioscience,
Biotechnology and Agrochemistry
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It is our pleasure to invite you to visit our booth in the event hall.

Date : March 27 (Fri) – March 29 (Sun), 2015 .
Place : Okayama University, Tsushima campus, Okayama
The 2015 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry

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– Pick Up Resource – AID system –
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To investigate the role of proteins regulated gene expression by chemicals in cells, and gene knock down method is widely applied. Here Dr. Kanemaki Masato offers a new reduction method of interested proteins, Auxin Inducible Degron (AID) system. By addition of auxin, you can degrade proteins in a very short period of time.

DNA Resource
pNHK60 (cat# RDB08468)
GFP fused with AID at the C-terminal end is expressed. This clone was used to demonstrate the decay of GFP by addition of auxin (ref. 1).

pMK106 (cat# RDB08469)
Centromere protein H (CENP-H) fused with the AID at the N-terminal end is expressed.

pMK107 (cat# RDB08470)
CENP-H fused with the AID at the C-terminal end is expressed.

Using these resources, the effectiveness of the AID system has been demonstrated (ref.1). Remarkably, both N-aid and C-aid cells ceased to grow immediately and subsequently started to die. Compared with the conventional method, it can efficiently remove the target protein.

Reference
1. Nishimura, K., et al. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat. Methods., 6, 917-922, 2009.
2. Kanemaki, M.T. Frontiers of protein expression control with conditional degrons. Eur. J. Physiol., 465, 419-425, 2013.

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– Focused Resource – Reference of materials –
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Vectors for specific expressed in cancer cells.

The HSV-TK gene in pTK5 plasmid was used to construct recombinantadenovirus having AFR promoter for the restricted expression of thymidine kinase in cancer cells.

Reference
Kurayoshi K, et al., Biochem Biophys Res Commun. 450(1):240-246 (2014).

DNA Resource
pTK5 (cat# RDB01027)

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– Link between KEGG and our genetic materials by the LinkDB
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The link between KEGG (Kyoto Encyclopedia of Genes and Genomes) database of pathways and orthologs among human, mouse and fission yeast and our resources are listed in the LinkDB by the efforts of Dr. Susumu Goto of the Bioinformatics Center, Institute for Chemical Research, Kyoto University.

Genetic resource of 16,000 of human, 11,000 of mouse and 5,000 of fission yeast can be searched through the KEGG. Our genetic resources can be searched associated with pathways and orthologs among human, mouse and fission yeast.

Please use the following address to enter a search window of the KEGG.

2015.03.18