Please review the QC test results indicated by icon below as well as clone information before placing your order.

tFucci(CA)5/pCS2

New color variant of Fucci: Fucci(CA)5.

Sequence (full)	RDB18221zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18221_B0Fbp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F (Pr0506) Region: CMV IE94 pro, SP6 pro, insert 5' Sequence file: RDB18221_B0Fba.seq
>D07168A4_B0Fb_3_pCMV-IE94_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN GNCTATATAA GCAATGCTCG TTTAGGGAAC    61 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG   121 CTACTTGTTC TTTTTGCAGG ATCCGCCACC ATGGAGAACG TCAGGAGAAA GACAGGCATT   181 CAGACGGAAA TGAAAACGAA GCTCCACATG GATGGTATGG TGAATGGACA CAGCTTTGAG   241 ATCAAAGGGG AGGGGAAGGG AAGCCCGTAC GAGGGGGTGC AGACAATGAA GCTGAAGGTT   301 ACTAAGGGCG CCCCACTTCC CTTCTCTATA GACATTCTTT TGCCTCAGTG CATGTATGGC   361 AGCAAACCTT TCATTAAATA TCCTGAGAAC ATCCCCGATT ATTTGAAACT CTCATTTCCA   421 GAGGGTATCA CTTGGGAGAG GACAATGACA TTCGAGGACG GCGCAGTATG CGATGTATCT   481 AATGATTCCA GACTGGTCGG CAATTGCTTT ATCTACACTG TGAAGTTTCA GGGAGTCAAT   541 TTTCCACTTG ATGGCCCCGT TATGCAAAAG AAGACCCGAG GCTGGGAACC CTCCACTGAA   601 GTGCTGTACG AATGTGATGG GTGGATGCGC GGGTTGGTGG ACATAGCTCT GAAACTGGAA   661 AACGGTGGAC ATTATATGTG TAATTTTAAG ACCACCTACA AATCCAAGAA AGGTCTTGAG   721 GTGCCACCTT ACCATTTCGT GGATCATAAA CTCGACCTGC TCAGTCATAA CACCGACGGA   781 NCCACCTTTG AGGAGTTCGA ACAANGTGAA ATCGCTCACG CGCACTTGAN TAAACTGGCC   841 GGANATNCAT CACACNGGCG GCCGCTCNNN ATGGANCANC GCCGCGNCAC CGANTTCTTC   901 GCGCGCCGCC GCCCCGGGCC CCCCCCGNAT CGCGCCGCCN AANCNGGNNN NNCGCACCCC 961 CNANCCCCNC CAGGNCNGCN NNNNNNGNCC CGGNCNNNCN NNNNNNNNN //
Primer: pGCAP_R (Pr0074) Region: T7 pro, insert 3' Sequence file: RDB18221_B0Fbb.seq
>D07168A4_B0Fb_3_pGCAP_R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNCG NNAATACGAC TCACTATAGG GCTGCAGAAT    61 CTAGATTACA GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA   121 TTTTCTTTAA TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA   181 CTAGATTCTG GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA   241 TGTTTCCTTT TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT   301 GCAGAAGGCT GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT   361 TTGATTTCTT CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT   421 GGATATCCCT TATTCAGGTT CTTTTCAGGC AGCAGGCTGT ACCTGGCCAC AGCCCCTGCG   481 TAGAGCTTGA CAGGGCGTCC TTCGGGCTTC TCCAGGATCT CGATCCGGTG GTCCACGAAG   541 TGGTAATCTG GCATCTTCTC CACCTTATTT TTGGGAATAT AAGAAGTGTT GAAATCGACT   601 CTGAGGTGAC CACCATCCTT CAACAGCAGG GCCATATTCA CGTCGCCCTT CAGCACTCCA   661 TCGTTTTTAT CGTCCTTGTA CATCACCTCG GTGGAAGGTT CCCATTTGAC TGTTTCCTTT   721 TGCATGACTG GGCCGTGGGG GGGGAANTTT CCGTTGAATT GTATTTTATA ATGGAAGCAG   781 TCCCCTTGAA CGCTGATATC GGANTCAACG GTTGANACGC CGCCNNCTTC ATAGGTCATT   841 GTTCTTTTCC ATGTGTATCC CCCGCCNCNG NAANGNNTGT TTGAAGAANT CTGGTATGTT   901 NNCGGGGNAC TNNGNNAACG CCCTATTGNC GNACTGAAAN GNTGGGNNCA GNNNATCNAA   961 NCTGNANNTC ANNGNNNTTN CNNNCNNNNN NNNNCNNGNN AANNGNNNGA NNNNNNNNNN //
Primer: Azalea5B_F (Pr0865) Region: insert mid Sequence file: RDB18221_B0Fbc.seq
>D07168A4_B0Fb_3_Azalea5B_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNTNCNA ATCNNAGAAA GGNCTTGAGG TGCCNCCTTA CCNTTTCGTG    61 GATCATAAAC TCGACCTGCT CAGTCATAAC ACCGACGGAG CCACCTTTGA GGAGTTCGAA   121 CAAGGTGAAA TCGCTCACGC GCACTTGAGT AAACTGGCCG GATATCCATC ACACTGGCGG   181 CCGCTCGAGA TGGAGCAGCG CCGCGTCACC GACTTCTTCG CGCGCCGCCG CCCCGGGCCC   241 CCCCGCATCG CGCCGCCCAA GCTGGCCTGC CGCACCCCCA GCCCCGCCAG GCCCGCACTC   301 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC   361 GGACGCGACC AGGCCAGGCC ACCGGCCCGC GCGGCCGCGC GGCTGTCGGT GGACGAGGTT   421 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG   481 ATAAAGAAAA CCGGTCGCGC CAAGCGCGGC AGCGGCGCCA CCAACTTCAG CCTGCTGAAG   541 CAGGCCGGCG ACGTGGAGGA GAACCCCGGC CCCCGCGAAT TCATGAGTAA ACTCCAGAAA   601 GGTGTGGAGA AAGAGATGAA GATTAAGCTC ACTATGAACT GCACCGTGAA CGAACGGAAC   661 TTTGTGATCA CAGGGCAGGG GGCTGGGGAA CCTTATGATG GAACTCAGAC ACTTTACCTG   721 ACAGTGGANG GCGGAAAGAC ACTGAACTTC AGCTTCGATG TACTGACCCC AGCCTTTCAG   781 TACGGCAATA GGGCGTTTAC CAAGTACCCC GGTAACATAC CAGACTTCTT CAAACAGACC   841 TTCAGTGNCG GGGGGANACA CATGGAAAAG AACNATGACC TATGAAGACG GCGGCGTCTC   901 AACCGTNGAG NNCGATATCA GCGTTCAAGG GGGNNNGCTN NCNTNNTAAA ATNCNANTCN   961 NNGNAAANNT NCCCCCCCNN NNNGNCCNAG NCATGNNAAN NNAAACNNNN NANNNGGNNN 1021 NCNNNNNNNN NN //
Primer: tFucci_f1 (Pr0571) Region: insert mid Sequence file: RDB18221_B0Fbd.seq
>D07168A4_B0Fb_3_tFucci_f1_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTCTCNNN NNCNNAAGNN AAAGAAAAAC CGGTCGCGCC    61 NNAGCGCGGC AGCGGCGCCA CCAACTTCAG CCTGCTGAAG CAGGCCGGCG ACGTGGAGGA   121 GAACCCCGGC CCCCGCGAAT TCATGAGTAA ACTCCAGAAA GGTGTGGAGA AAGAGATGAA   181 GATTAAGCTC ACTATGAACT GCACCGTGAA CGAACGGAAC TTTGTGATCA CAGGGCAGGG   241 GGCTGGGGAA CCTTATGATG GAACTCAGAC ACTTTACCTG ACAGTGGAGG GCGGAAAGAC   301 ACTGAACTTC AGCTTCGATG TACTGACCCC AGCCTTTCAG TACGGCAATA GGGCGTTTAC   361 CAAGTACCCC GGTAACATAC CAGACTTCTT CAAACAGACC TTCAGTGGCG GGGGATACAC   421 ATGGAAAAGA ACAATGACCT ATGAAGACGG CGGCGTCTCA ACCGTTGAGT CCGATATCAG   481 CGTTCAAGGG GACTGCTTCC ATTATAAAAT ACAATTCAAC GGAAAGTTCC CCCCCCACGG   541 CCCAGTCATG CAAAAGGAAA CAGTCAAATG GGAACCTTCC ACCGAGGTGA TGTACAAGGA   601 CGATAAAAAC GATGGAGTGC TGAAGGGCGA CGTGAATATG GCCCTGCTGT TGAAGGATGG   661 TGGTCACCTC AGAGTCGATT TCAACACTTC TTATATTCCC AAAAATAAGG TGGAGAAGAT   721 GCCAGATTAC NACTTCGTGG ACCACCGGAT CGAGATCCTG GAGAAGCCCG AAGGACGCCC   781 TGTCAAGCTC TACGCAGGGG CTGTGGCCAG GTACAGCCNG CTGCCTGAAA AGAANCTGAA   841 TANNGGATAT CCATCACACT GGCGGCCGCT CGAGATGANT CCCAGTATGA AGCANAANCA 901 AGAANNAAAN TCAAAGANAN TATAAANNAA TAGTTNCTGN CCNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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