Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSIV-TRE-HPV-E6/E7-UbC-KT

Lentivirus vector HPV16 E6 and E7 with Tet-responsive promoter.

Sequence (full)	RDB17719zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kurita R. et al. PLos One, 8(3): e59890, 2013) Additional terms and conditions: The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kurita R. et al. PLos One, 8(3): e59890, 2013)。 付加的提供条件： 本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17719_A9L9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_v2_F (Pr0303) Region: tight TRE pro (7xtetO), attB1, insert 5' Sequence file: RDB17719_A9L9h.seq
>D06794A2-1_A9L9_cPPT+CTS_v2_F_A07_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGA NTACNAAAAC NAATTACAAA AATTCNAAAT TTTCGGGTTT    61 ATTACAGGGN CAGCAGAAAT TCACTTTGAA TTCGGCCCTT TCGTCTTCAA TCGAGTTTAC   121 TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGAT   181 GTCGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA   241 TAGAGAACGT ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGTTTATC   301 CCTATCAGTG ATAGAGAACG TATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG   361 TCGAGGTAGG CGTGTACGGT GGGAGGCCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA   421 GATCGCCGCT AGCATCACAA GTTTGTACAA AAAAGCAGGC TCCACCATGT TTCAGGACCC   481 ACAGGAGCGA CCCAGAAAGT TACCACAGTT ATGCACAGAG CTGCAAACAA CTATACATGA   541 TATAATATTA GAATGTGTGT ACTGCAAGCA ACAGTTACTG CGACGTGAGG TATATGACTT   601 TGCTTTTCGG GATTTATGCA TAGTATATAG AGATGGGAAT CCATATGCTG TATGTGATAA   661 ATGTTTAAAG TTTTATTCTA AAATTAGTGA GTATAGACAT TATTGTTATA GTTTGTATGG   721 AACAACATTA GAACAGCAAT ACAACAAACC GTTGTGTGAT TTGTTAATTA NGTGTATTAA   781 CTGTCAAAAG CCACTGTNNC CTGAAGAAAA GCAAANNNAT CTGGACNAAA AGCNANNNTT   841 CCATAATATA AGGNGTCGGN GGANNNNNCN ATGTATGTCT TGNTGCANNT CATCAAGAAC 901 NCNNNNAGAA ACCNANCNNN NNNCANNNNN GNNNNTACAC NNACNTNNNN NNNNNN //
Primer: UbC-N_R (Pr0262) Region: UbC pro, attB2, insert 3' Sequence file: RDB17719_A9L9i.seq
>D06794A2-1_A9L9_UbC-N_R_B07_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN NTCCGGGCGG ANGNNCAGGN NGCTCGCTGC    61 GCCCTTCGTC TGACGTGGCA GCGCTCGCCG TGNGGAGGGG GGCGCCCGCG GGGGGCGCCA   121 AAACCCGGCG CGGANGCCAG ATCTCCCGAT CTCGAGACCG GTAGCATCAC CACTTTGTAC   181 AAGAAAGCTG GGTTATGGTT TCTGAGAACA GATGGGGCAC ACAATTCCTA GTGTGCCCAT   241 TAACAGGTCT TCCAAAGTAC GAATGTCTAC GTGTGTGCTT TGTACGCACA ACCGAAGCGT   301 AGAGTCACAC TTGCAACAAA AGGTTACAAT ATTGTAATGG GCTCTGTCCG GTTCTGCTTG   361 TCCAGCTGGA CCATCTATTT CATCCTCCTC CTCTGAGCTG TCATTTAATT GCTCATAACA   421 GTAGAGATCA GTTGTCTCTG GTTGCAAATC TAACATATAT TCATGCAATG TAGGTGTATC   481 TCCATGCATG ATTACAGCTG GGTTTCTCTA CGTGTTCTTG ATGATCTGCA ACAAGACATA   541 CATCGACCGG TCCACCGACC CCTTATATTA TGGAATCTTT GCTTTTTGTC CAGATGTCTT   601 TGCTTTTCTT CAGGACACAG TGGCTTTTGA CAGTTAATAC ACCTAATTAA CAAATCACAC   661 AACGGTTTGT TGTATTGCTG TTCTAATGTT GTTCCATACN AACTATNACA ATAATGTCTA   721 TACTCACTAA TTTTAGAATA AAACTTTANA CATTTATCAC ATACAGCATA TGGATTCCCA   781 TCTCTATATA CTATGNATAA ATCNCGAAAA GCAAANTCAT ATACNNCACG TCNCANNAAC   841 TGNTGCNNGC ANTACACACN TNCTAANATT ATATCANGNA TAGTNGTTNG CANCTCTNNN 901 NATAACNNNN NNNNNN //
Primer: UbCpro_F (Pr0158) Region: hKO 5' Sequence file: RDB17719_A9L9j.seq
>D06794A2-1_A9L9_UbCpro_F_C07_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NNNNNGTCCG CTAAATTCTG GCCGTTTTTG    61 GCTTTTTTGT TAGANCGGAC CGTGTTTAGC GCTACCGGTC CACCATGGTG AGCGTGATCA   121 AGCCCGAGAT GAAGATGAAG TACTTCATGG ACGGCAGCGT GAACGGCCAC GAGTTCACCG   181 TGGAGGGCGA GGGCACCGGC AAGCCCTACG AGGGCCACCA GGAGATGACC CTGAGGGTGA   241 CAATGGCCAA GGGCGGCCCC ATGCCCTTCA GCTTCGACCT GGTGAGCCAC ACCTTCTGCT   301 ACGGCCACAG GCCCTTCACC AAGTACCCCG AGGAGATCCC CGACTACTTC AAGCAGGCCT   361 TCCCCGAGGG CCTGAGCTGG GAGAGGAGCC TCCAGTTCGA GGACGGCGGC TTCGCCGCCG   421 TGAGCGCCCA CATCAGCCTG AGGGGCAACT GCTTCGAGCA CAAGAGCAAG TTCGTGGGCG   481 TGAACTTCCC CGCCGACGGC CCCGTGATGC AGAACCAGAG CAGCGACTGG GAGCCCAGCA   541 CCGAGAAGAT CACCACCTGC GACGGCGTGC TGAAGGGCGA CGTGACCATG TACCTGAAGC   601 TGGCCGGCGG CGGCAACCAC AAGTGCCAGT TCAAGACCAC CTACAAGGCC GCCAAGAAGA   661 TCCTGAAGAT GCCCCAGAGC CACTTCATCG GCCACAGGCT GGTGANGAAG ANCGANGGCA   721 ACATCACCGA GCTGGTGGAN GANGCCGTGG CCCACTGCGN NGGCAGANGA AGTCTTCTAA   781 CATGCGGTGA NGTGNANGAN AATCNNGGCC CTATGTCTAG ACTGGNCAAN ANCAAAGNCA   841 TAAACGGNGC TCNGGAATTA CTCANNNGAN NNNGNATCNN ANNNCCNNNN GANNNNGAAA 901 NNCGCNCAAA ANNNGGNANT NNANCANNNN ANCNNNNNNN NNN //
Primer: mKO2-M_F (Pr0578) Region: hKO 3', T2A, rtTA-Advenced Sequence file: RDB17719_A9L9k.seq
>D06794A2-1_A9L9_mKO2-M_F_D07_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG TNNNNNNNNN CCTACNAGGC CGCCAAGAAG    61 ANCCTGAAGA TGCCCCANNG CCACTTCATC GGCCACAGGC TGGTGAGGAA GACCGAGGGC   121 AACATCACCG AGCTGGTGGA GGACGCCGTG GCCCACTGCG AGGGCAGAGG AAGTCTTCTA   181 ACATGCGGTG ACGTGGAGGA GAATCCCGGC CCTATGTCTA GACTGGACAA GAGCAAAGTC   241 ATAAACGGCG CTCTGGAATT ACTCAATGGA GTCGGTATCG AAGGCCTGAC GACAAGGAAA   301 CTCGCTCAAA AGCTGGGAGT TGAGCAGCCT ACCCTGTACT GGCACGTGAA GAACAAGCGG   361 GCCCTGCTCG ATGCCCTGCC AATCGAGATG CTGGACAGGC ATCATACCCA CTTCTGCCCC   421 CTGGAAGGCG AGTCATGGCA AGACTTTCTG CGGAACAACG CCAAGTCATT CCGCTGTGCT   481 CTCCTCTCAC ATCGCGACGG GGCTAAAGTG CATCTCGGCA CCCGCCCAAC AGAGAAACAG   541 TACGAAACCC TGGAAAATCA GCTCGCGTTC CTGTGTCAGC AAGGCTTCTC CCTGGAGAAC   601 GCACTGTACG CTCTGTCCGC CGTGGGCCAC TTTACACTGG GCTGCGTATT GGANGAACAG   661 GAGCATCAAG TAGCAAAAGA GGAAAGAGAG ACACCTACCA CCGATTCTAT GCCCCCACTT   721 CTGANACAAG CAATTGAGCT GTTCGACCGG CAGGGAGCCG AACCTGCCTT CCTTTTCGGC   781 CTGNAACTAA TCATATGTGG CCNGGANAAN CAGCNAAAGT GCGAAAGCGG CGGGCCGGCC   841 NANGCCCTTG ACGATTTTGA CTTANACATG CTCCCAGCCN ATGCCCTNNN CGACTTNGNN 901 CNTGNNATGN TGNNNGNNNN CGCNNNNGNN NANTTNNNNN NNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5' LTR, HIV-1 psi Sequence file: RDB17719_A9L9l.seq
>D06794A2-1_A9L9_CMV-Forward_E07_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTN NNGCNNNTGG GCGGTANGCG TGTANGGTGG GNNGTCTATA    61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG   121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT   181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT   241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA   301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG   361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG   421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC   481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG   541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA   601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA   661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG   721 CTTTAGACAA GATAGAGGAA GAGCNAAACA AAAGTNAGAN CACCGCACAG CANGCGGCCG   781 CNNATCTTCA GANCTGGANG ANGANATATG AGGGANNNTT GGAGAAGTGA ATTATATAAA   841 TATAAAGTAN TAAANTTGAA NNNTTANGNN TANCNCCCAC CNAGGCAAAN ANAAGANNGN 901 NGCNNANNNN AAAAANNNAN CNNNNNNNNN N //
Primer: BGH_rev2 (Pr0606) Region: 3' LTR, loxP, PRE Sequence file: RDB17719_A9L9m.seq
>D06794A2-1_A9L9_BGH_rev2_F07_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGGGNNGG GCNNNNNNNN GATGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGNTCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT   121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT   181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA   241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA ATAACTTCGT   301 ATAATGTATG CTATACGAAG TTATGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC   361 CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG   421 TCTTAAAGGT ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG   481 CCAGGCACAA TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC   541 TAGGTCTCGA CGGTATCGAT GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG   601 GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC   661 CGCTGGATTG ANGGCCGAAN GGANGTANCA GAANGACGTC CCGCGCAGAA TCCAGGTGGC   721 AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTNNNC GACNACACCA CGGAATTGTC   781 AGTGCCCNAC AGCCGANCCC CTGTCCNGCA NCGGGCAAGG CANGCGGCGA TGANTTCCGC   841 CGNGNNNATA NGGANGGGGA AAGCGAAAGT CCCNGAAANG ANCTGANNNG NGGNGGCANN   901 GCCCNNNNCN GNGGGGGGTT GCGNCANCAA ACNCAGNGNN NNNCACGNCN NCNNNGCNNG 961 NNNNNNGNNN NNNANNNNNN NTNAAGNNNN NGNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, EM7 pro, Zeo Sequence file: RDB17719_A9L9n.seq
>D06794A2-1_A9L9_SV40pro_ori_F_G07_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCTGCCTC TGNNCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC   181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC   241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG   301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG   361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG   421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG   481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC   541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC   601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGNTGATC   661 CTCCAGCGCG GGGNTCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT   721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA   781 CTGCATTCTA GTTGTGGTTT GTCCNAACTC ATCAATGTAT CTTATCATGT CTGTATANNN   841 NCNANCTCTA NCTNGAGCTT GGNGNAATCN NNGNCATANC NGTTTTCCNN NNNGAAATNG 901 NTATCCNCNC ACNANTTNCN CNCNACNNNC NANNNNGNNN CATNAANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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