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pCMV-MvChR1-Venus

Mammalian expression vector encoding Met1-Arg323 of channelrhodopsin 1 from Mesostigma viride (MvChR1).

Catalog number RDB16786
Resource name pCMV-MvChR1-Venus
Clone info. Mammalian expression vector encoding Met1-Arg323 of channelrhodopsin 1 from Mesostigma viride (MvChR1) tagged with Venus at the C-terminus under CMV promoter.
Comment MvChR1 cDNA was a gift from Dr. JL. Spudich (Govorunova, G. et al., mBio, 2(3): e00115-11, 2011). Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Mesostigma viride MvChR1 cDNA
Depositor|Developer Ishizuka, Toru |
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External Database
Mesostigma viride MvChR1
            Reference sequence
              JF922293
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, S. et al., Photochem. Photobiol. Sci., 15: 365-374, 2016) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Watanabe, S. et al., Photochem. Photobiol. Sci., 15: 365-374, 2016)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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            RDB16786 pCMV-MvChR1-Venus Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pCMV-MvChR1-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16786).

            Reference section:

            Watanabe, S., Ishizuka, T., Hososhima, S., Zamani, A., Hoque, M.R., Yawo, H., The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1). Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PMID 26853505. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Watanabe, S., The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1). Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PMID 26853505. [link to RRC of NBRP]

            2023.11.23

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