RDB16767 - pDEST/mCherry-CeRhoA

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pDEST/mCherry-CeRhoA

Expression vector of C. elegans RhoA.

Catalog number	RDB16767
Resource name	pDEST/mCherry-CeRhoA
Clone info.	Expression vector of C. elegans RhoA fused with mCherry at N-terminus.
Comment	Expression was confirmed by the depositor with immunofluorescence. Insert gene, from C. elegans cDNA library by PCR amplification.Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone	pcDNA-DEST40 (plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	C. elegans RhoA cDNA
Depositor\|Developer	Sako, Yasushi \| Abe, Mitsuhiro \|

Other clones in our bank

C. elegans RhoA and ortholog

External Database
C. elegans RhoA

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012) is requested.

Ordering Please visit Information of Request for Distribution.[link]
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012) is requested.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献（Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012）の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16767	pDEST/mCherry-CeRhoA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16767_A8Kmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Red-C_F Sequence file: RDB16767_A8Kma.seq
>D05982A4_A8Km_2_Red-C_F_G09_21_ABI24.ab1 1 CAGTCAATCA AGTTGGACTC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG 61 AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG AAGCTTATGG 121 CTGCGATTAG AAAGAAGTTG GTGATCGTCG GCGACGGAGC CTGCGGTAAA ACTTGCCTGC 181 TCATCGTTTT CTCAAAGGAT CAGTTCCCAG ACGTGTATGT GCCGACTGTT TTCGAGAATT 241 ATGTTGCCGA CATTGAAGTT GACGGAAAGC AGGTCGAACT TGCTCTATGG GATACAGCTG 301 GACAAGAGGA CTATGATCGT CTGCGTCCAC TCTCTTATCC GGATACTGAC GTCATTCTGA 361 TGTGCTTCTC AATTGATTCA CCCGATTCAC TGGAGAATAT TCCAGAAAAA TGGACACCAG 421 AAGTCAGGCA TTTCTGTCCA AATGTTCCGA TTATTTTGGT CGGAAATAAA CGAGATCTTC 481 GTAGTGATCC ACAGACTGTT CGAGAATTGG CAAAAATGAA ACAGGAGCCC GTGAAGCCTG 541 AGCAGGGACG AGCAATTGCT GAGCAAATTG GAGCATTTGC ATATTTGGAG TGCTCTGCGA 601 AGACTAAGGA CGGAATTCGT GAGGTATTCG AGAAGGCGAC ACAGGCGGCA TTGCAACAGA 661 AGAAGAAAAA GAAGAGCAAG TGCATGATTT TGTAACTCGA GGTTTAAACC CGCTGATCAG 721 CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT 781 TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC 841 ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG 901 GAGGATTGGG AAGACAATAG CAGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG 961 CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA 1021 GCGCGGCGGG TGTGGTGGTT TACGCGCAGC GTGACCGCTA CACTTTGCCA GCGCCTAGCG 1081 CCCGCCTCTT TCGCTTTCTT CCATTCGTTC TCGCCACGTT CGCAGCTTTC CCCGATCAAG 1141 CTCTTAAAAT TCG //
Primer: CMV_Forward Sequence file: RDB16767_A8Kmb.seq
>D05982A4_A8Km_2_CMV_Forward_E09_15_ABI24.ab1 1 TGGTACACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA 121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA TCAGGTACCA TGGTGAGCAA 181 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA 241 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA 301 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA 361 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT 421 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT 481 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT 541 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA 601 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG 661 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC 721 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT 781 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG 841 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGAAG CTTATGGCTG CGATTAGAAA 901 GAAGTTGGTG ATCGTCGGCG ACGGAGCCTG CGGTAAAACT TGCCTGCTCA TCGTTTTCTC 961 AAAGGATCAG TTCCCAGACG TGTATGTGCC GACTGTTTTC GAGAATTATG TTGCCGACAT 1021 TGAAAGTTGA CGGGAAAGCA GGTCGAAACT TTGCTCTATG GGATACAGCT GGGACAAGAG 1081 GACTATGATC GTCTGCGTCC AACTCCTCTT AATCGCATAC TGAACGGTTC AATCCTGAAT 1141 GTGCATTCCC TCCAACTTGG AATTTCGAC //
Primer: SV40pro_F Sequence file: RDB16767_A8Kmc.seq
>D05982A4_A8Km_2_SV40pro_F_H09_24_ABI24.ab1 1 CCTGTCCCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT 121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC 661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGC GAAATGACCG ACCAAGCGAC 961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC CTTCTATGAA AGGTTGGGCT 1021 TCGGAATCGT TTTCCCGGGG ACGCCGGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 1081 TGGAGTTCTT TCGCCCACCC CGACCTGGTT AATGGCCAGC CTATAATTGA TTACGAATAA 1141 AAGCAATAGC CAATCCACCG AAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl