Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDEST/mCherry-PIP5KB

Expression vector of human phosphatidylinositol 4-phosphate 5-kinase beta (PIP5K beta).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012）の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16765_A8Kmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Red-C_F Sequence file: RDB16765_A8Kma.seq
>D05982A2_A8Km_2_Red-C_F_G08_20_ABI24.ab1     1 CTGCCACTCA GTTGGAATCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA    61 ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGG GAGGTGGAGG   121 TTCTAGAATG TCTTCTGCTG CTGAAAATGG AGAGGCAGCA CCTGGAAAAC AAAATGAAGA   181 AAAAACCTAT AAAAAGACTG CATCATCTGC TATTAAAGGT GCTATTCAGC TGGGAATAGG   241 ATACACAGTG GGTAATCTCA CTTCCAAGCC AGAACGAGAT GTTCTTATGC AAGACTTTTA   301 TGTGGTGGAA AGTGTGTTCC TACCCAGCGA AGGGAGCAAT CTGACCCCAG CACATCACTA   361 CCCAGACTTT AGATTTAAGA CATACGCTCC ATTAGCATTC CGATATTTCA GAGAACTTTT   421 TGGTATCAAG CCTGATGATT ACTTGTATTC CATCTGCAGT GAACCTCTAA TAGAACTGTC   481 TAACCCTGGA GCCAGTGGAT CCTTGTTTTT TGTGACCAGT GATGATGAAT TTATCATCAA   541 AACAGTTCAG CACAAAGAAG CTGAGTTTCT TCAGAAGCTA CTGCCAGGCT ATTACATGAA   601 TTTAAACCAG AATCCAAGGA CTCTTTTGCC AAAATTTTAC GGACTGTATT GTATGCAATC   661 AGGAGGCATT AATATCAGGA TTGTGGTGAT GAACAACGTT TTGCCACGCT CCATGAGAAT   721 GCACTTTACA TATGACTTGA AAGGCTCAAC GTATAAGCGA AGAGCATCCC GTAAAGAGAG   781 AGAGAAATCC AACCCCACAT TTAAGGACTT AGATTTCCTG CAAGACATGC ACGAAGGGTT   841 GTATTTTGAT ACGGAAACAT ACAACGCGCT TATGAAAACA CTTCAGAGAG ACTGCCGGGT   901 GCTAGAAAGC TTCAAGATCA TGGATTATAG CCTTCTGTTG GGAATTCATT TCCTGGACCA   961 TTCCCTCAAA GAGAAAGAGG AGGAGACCCC ACAAAATGTG CCCTGATGCT AAGCGGGACT  1021 GGGATGCAGA AGGTTCTTCT ACTCAACAGC CATGGCAATC TATCCAGGGT CCAGGGAATC  1081 TGGAGATGGG ATAATCACAG AGCACCCAGA CGCAATGGCA GCAGTTCAGC CTTAAAAGCC 1141 AATAGGGGGG AGAAAAAATC T //
Primer: F1ori_F Sequence file: RDB16765_A8Kmb.seq
>D05982A2_A8Km_2_f1ori_F_B11_02_ABI08.ab1     1 GAGGGGCCGT TAGGGCGCTG GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC    61 GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TGGGGATACC CCCTAGAGCC CCAGCTGGTT   121 CTTTCCGCCT CAGAAGCCAT AGAGCCCACC GCATCCCCAG CATGCCTGCT ATTGTCTTCC   181 CAATCCTCCC CCTTGCTGTC CTGCCCCACC CCACCCCCCA GAATAGAATG ACACCTACTC   241 AGACAATGCG ATGCAATTTC CTCATTTTAT TAGGAAAGGA CAGTGGGAGT GGCACCTTCC   301 AGGGTCAAGG AAGGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT AGAAGGCACA   361 GTCGAGGCTG ATCAGCGGGT TTAAACCTCG AGTTATAAAT AGACGTCAAG CACAGAAGCA   421 TTGTCATCCT GCACTTCCAG AGTGTTGGGC TCAGCGGTCA AGTAGATGGT CCCCTCTTCC   481 AAGGTAAATG TTGAACTGGA AGGTAACCCT TTGCTGTTTG AATAGAGTGT AGGCCTGTCG   541 TGTGGATAGT GCTCATTGAC GTATAAGGAA GAAGATGAAA TTGTGGTTGC CAAGGAAGCA   601 GCTTCAAACA GTGATGGAGT GCTAGGGACC AGGTCTGGCC TGTGTCGGGA TCCCAGGGCT   661 TCTTCATCAA GGCTGCTAAA ACTTTGTCCT TCAGTCAGCA AATCCCGCTT CTCATCCTTC   721 CATTCTTGGC TAATTGAGGA CACAATCTCC TGTGAAGTGG CCTTTAGGGC GGCGATTGAA   781 TTGCACCGTT TCTTAGACGG TGAAGCCTTC AAAGCTTGAA TTTTCTTGAA AACTCTGGAA   841 TTCATGAACT TAAGAAATCT GTCTGCATAA AAGCTTGGTC TATGAACAGA AACAGTGTCC   901 CCATCATAAA CAAGAGCTTT CCAGGAAATG TTCTAACTTC TTCATTAACC TATATGATTG   961 CAGAATGTCA TAATGCCCAT AAATAAAAGT AGTTTTTTCT CCCCTATGGC TTTTAGCTGG  1021 AATGCCCTCC CATTGTGTCT GGGATTCTCT GTGATTATCC CATCTCCAGA TTCCCTGGAC  1081 CTGGATAGGA TTTCATGGCT GTGGAGTAGA GAACATCTGC ATTCCAGTCG CTTAGCATCA 1141 AGGCACATTT GGTTGGGGTC TCCTTTCCTT TCCT //
Primer: CMV_Forward Sequence file: RDB16765_A8Kmc.seq
>D05982A2_A8Km_2_CMV_Forward_E08_14_ABI24.ab1     1 TGAACACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC   121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GTTAAGCTAT CAGGTACCAT GGTGAGCAAG   181 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG   241 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG   301 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC   361 ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC   421 CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC   481 GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC   541 TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG   601 ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC   661 GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC   721 ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG   781 GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC   841 CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGGGAG GTGGAGGTTC TAGAATGTCT   901 TCTGCTGCTG AAAATGGAGA GGCAGCACCT GGAAAACAAA ATGAAGAAAA AACCTATAAA   961 AAGACTGCAT CATCTGCTAT TAAAGGTGCT ATTCAGCTGG GAATAGGATA CACAGTGGGT  1021 AATCTCACTT CCAAGCCCAG AACGAGATGT TTCTTATGCA GACTTTTTTA TGTGGAGAAA  1081 GTGTGTTCCT ACCCCAGCGA AGAAGCCATC CTGGACCCCA GCCACATTCA ACTACCCCAG 1141 AAACCTTTTA GGAAATTTTA AGG //
Primer: SV40pro_F Sequence file: RDB16765_A8Kmd.seq
>D05982A2_A8Km_2_SV40pro_F_H08_23_ABI24.ab1     1 CCCTTGGTCC TGACCTTTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA    61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT   121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA   661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG   721 TGGAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG   901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTTC GCGAAATGAC CGACCAAGCG   961 ACGCCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCG CTTCTATGAA GTTGGGCTTC  1021 GGATCGTTTT TCGGGACGCG GCTGATGATC CTCAAGCGCG GGGATCTCAT GCCTGGAAGT 1081 CTCGCCTACT CAACTTGGTT AATGCCAGCT AATAATTGGT TTAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl