Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGedit

Plasmid vector for high efficiency genome editing oligo DNA containing target sequence.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagasaki, A et al. Plasmid. 98, 37-44, 2018) is requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Nagasaki, A et al. Plasmid. 98, 37-44, 2018）の引用を必要とする。
提供依頼	提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16763_A8Kkp1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: T7 pro,U6 pro,gRDA scaffold,T3 pro Sequence file: RDB16763_A8Kke.seq
>D05970A1_A8Kk_2_M13_-40_A04_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNANTNN CTATAGGGCG AATTGGGTAC CAAGGTCGGG    61 CAGGAAGAGG GCCTATTTCC CATGATTCCT TCATATTTGC ATATACGATA CAAGGCTGTT   121 AGAGAGATAA TTAGAATTAA TTTGACTGTA AACACAAAGA TATTAGTACA AAATACGTGA   181 CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT TAAAATGGAC   241 TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG   301 AAAGGACGAA ACACCGGAGA CGAATTCGTC TCTTTAAGAG CTAGAAATAG CAAGTTTAAA   361 TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTTCTAGA   421 GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTTCGAG   481 CTTGGCGTAA TCANGNNCAT NNCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC   541 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA   601 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA   661 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC   721 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC   781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCANGG GATAACGCAN GAAAGAACAT   841 GTGAGCAAAA GGCCAGCNAA AGGNCAGGAA NCGTAAAAAN GCCGCGTTGC TGGCGTTTTT   901 CCNTANGCNN CGCCCCCNNG ANNANCATCN CAAAAATCNA CGCTCNAGTC ANANGNGGNN   961 AANNCCGACN NGANTATAAA GANNCCANNN GTTTCCNNNT NNAANCNCCC NNNNGCNNTN 1021 NNNNGNNNNN ANNNNNNNNN N //
Primer: CMV_Forward (Pr0016) Region: CMV pro,Cas9 5' Sequence file: RDB16763_A8Kkf.seq
>D05970A1_A8Kk_2_CMV Forward_B04_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNATGGG CGGTAGGCGT GTANCGGTGG    61 GAGGNNNNNN NAAGCANNAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC ATGGACAAGA   121 AGTACAGCAT CGGCCTGGAC ATCGGCACCA ACTCTGTGGG CTGGGCCGTG ATCACCGACG   181 ACTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA CACCGACCGG CACAGCATCA   241 AGAAGAACCT GATCGGCGCC CTGCTGTTCG GCTCTGGCGA AACAGCCGAG GCCACCCGGC   301 TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA CCGGATCTGC TATCTGCAAG   361 AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT CTTCCACAGA CTGGAAGAGT   421 CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC CATCTTCGGC AACATCGTGG   481 ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA CCTGAGAAAG AAGCTGGCCG   541 ACAGCACCGA CAAGGCCGAC CTGAGACTGA TCTACCTGGC CCTGGCCCAC ATGATCAAGT   601 TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA CAACAGCGAC GTGGACAAGC   661 TGTTCATCCA GCTGGTGCAG ATCTACAATC AGCTGTTCGA GGAAAACCCC ATCAACGCCA   721 GCAGAGTGGA CGCCAANGCC ATCCTGAGCG CCNNACTGAG CAAGAGCAGA CGGCTGGAAA   781 ATCTGATCGC CCANCTGCCC GGCGAGAAGC GGAATGGCCT GTNCGGCNNC CTGATTGCCC   841 TGANCCTGGG CCNGANCCCC AACTTCNNNA GCAACTTCNN NNNTGNNNGA NGANGCCNAA   901 CTGCAGCTGA NCNNGGACAC CTACGACGAN NACCTGNNNN NNCTGCTGGC CCNNATCNGN   961 NGANCANNAC NNCGACCTGT TTCNNGNCNN CNAGAANCNT GTNNNANNNN NNNCNNGCNN 1021 ANCNNCNTNC NNNANNNNNN NNNN //
Primer: EGFP-N (Pr0027) Region: T2A,SV40 NLS,Cas9 3' Sequence file: RDB16763_A8Kkg.seq
>D05970A1_A8Kk_2_EGFP-N_D04_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTCGCCCT TGCTNNCNNT GGTGGCGANC GGTATCGATC    61 GTGGGCCAGG ATTCTCCTCG ACGTCACCGC ATGTTAGCAG ACTTCCTCTG CCCTCTCCAC   121 TGCCGTTAAC CACTTTGCGC TTTTTCTTGG GGTCGCCTCC CAGCTGAGAC AGGTCGATCC   181 GTGTCTCGTA CAGGCCGGTG ATGCTCTGGT GGATCAGGGT GGCGTCCAGC ACCTCTTTGG   241 TGCTGGTGTA CCTCTTCCGG TCGATGGTGG TGTCAAAGTA CTTGAAGGCG GCAGGGGCTC   301 CCAGGTTGGT CAGGGTAAAC AGGTGGATGA TATTCTCGGC CTGCTCTCTG ATGGGCTTAT   361 CCCGGTGCTT GTTGTAGGCG CTCAGCACCT TGTCCAGGTT GGCATCGGCC AGGATCACGC   421 GCTTGGAGAA CTCGCTAATC TGCTCGATGA TCTCGTCCAG GTAGTGCTTG TGCTGTTCCA   481 CAAACAGCTG TTTCTGCTCA TTATCCTCGG GGGAGCCCTT CAGCTTCTCA TAGTGGCTGG   541 CCAGGTACAG GAAGTTCACA TATTTGGAGG GCAGGGCCAG CTCGTTTCCC TTCTGCAGTT   601 CGCCGGCAGA AGCCAGCATC CGCTTCCGGC CGTTTTCCAG CTCGAACAGG GAGTACTTAG   661 GCAGCTTGAT GATCAGGTCC TTTTTCACTT CTTTGTAGCC CTTGGCTTCC AGAAAGTCGA   721 TGGGATTCTT CTCGAAGCTG CTTCTTTCCA TGATGGTGAT CCCCANCAGC TCTTTCACAC   781 TCTTCAGTTN CTTGGACTTG CCCTTTTCCN CTTTGGCCAC CACCAGCACA GANTANGCCA   841 CGGNGGGNNT GTCAAAGCCG CCGTACTTCT TANGGNNCCN ATCCTTCTTT CTGGNNATCA   901 GCTTGNCNNA NTNNCTCTNN GGCAGGANAN NNTCTTNGCT GAANNCGNNN GNCTGNNNNN 961 CGNNCTTTTT NNACNANATT CNCTTGGGGN ANGGNNAGCN NTTTNNNNNN NNNN //
Primer: pAxEF R2 (Pr0059) Region: SV40 pA,Bsr,EGFP 3' Sequence file: RDB16763_A8Kkh.seq
>D05970A1_A8Kk_2_pAxEF-R2_D03_12_ABI24.ab1     1 NNNNNNNNNN NNGNTTNNNT TNNGANNTTG TGANGCTATT GCTTTATTTG TAACCATTAT    61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG   121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA   181 TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACGGACGAGT ACCTTAATTT   241 CGGGTATATT TGAGTGGAAT GAGTTCTTCA ATCGTAGTTT TGACTAACTT GCCATTCATT   301 TCTATTAACA CAAAACAATC TGGTGCATAG TCTGAAATCA ACTCCCTACA CATACCACAA   361 GGACTTACCA CTCGAATACT TCTATCTACT TCGTCAGAAT AAGGGTGTCT AACAGCTACA   421 ATCGTGTCAA AATCCTTTTG TCCATTCGAA ACTGCACTAC CAATCGCAAT GGCTTCTGCA   481 CAAACAGTTA CTCGTCCTAT ATACGCTTCA ATATGTACTG CCGAAATGAT TTCTCCTGTT   541 TTCGTACGAA TTGCCGCTCC CACATGATGT TTATTATCCT CATAAAGCAT TGTAATCTTC   601 TCTGTCGCTA CTTCTACTAA TTCTAGATCC TGTTGAGAAA TGTTAAATGT TTTCATTGGA   661 TCTGAGTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC   721 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC   781 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG   841 TGGTCGGCGA NCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAN NTTCACCTTG   901 ATGNCGTTCT TCNGCTTGNC NGNCATGANA TAGANGTTGT NNNTGTTGTA NTTGTACTCC   961 AGCTTGNGCC CCNNGANGTT GNCGNCCNNC NNNANNNNNA NGNCNNTCNG CNNNANGCNG 1021 NNNCANCNNG NNNNNNNNNN //
Primer: Amp_R (Pr0008) Region: CMV pro,Cas9 5' Sequence file: RDB16763_A8Kki.seq
>16763_16763_B2G6F_Amp_R_A06_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNANNNN NNNNNNNGGG TTATTGTCTC NTGAGCGGAN    61 ACATATGCCA AGTACGCCCC CNATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA   121 TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT   181 CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA   241 CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA   301 AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG   361 TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG   421 CTAGCATGGA CAAGAAGTAC AGCATCGGCC TGGACATCGG CACCAACTCT GTGGGCTGGG   481 CCGTGATCAC CGACGACTAC AAGGTGCCCA GCAAGAAATT CAAGGTGCTG GGCAACACCG   541 ACCGGCACAG CATCAAGAAG AACCTGATCG GCGCCCTGCT GTTCGGCTCT GGCGAAACAG   601 CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA CACCAGACGG AAGAACCGGA   661 TCTGCTATCT GCAAGAGATC TTCNGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC   721 ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA GCACGAGCGG CNCCCCATCT   781 TCNGCAACAT CGTGGACGAN GTGGCCTANC ACGAGAANNT ANCCCACCAT CTACNACCTG   841 ANAAAGAAGC TGGCCGACAG CANCGACAAN GNCCGACCTG AGACTGATCT ACNNGGNCCN   901 GGCCCANATG ATCNAGTTCC GGGGCCACTT CCTGNTCGAG GGCGANCNTG AACCCCNACN   961 NCNNCNANNN NGNANNANCT GTNCATNCNG CTNGGTGCNN NNCTACNANT NNNNNTNTNC 1021 NANGNAAAAC CNNNNTNNNN NNNNNN //
Primer: FP-N_F1 (Pr0920) Region: EGFP 3',Bsr 5' Sequence file: RDB16763_A8Kkj.seq
>16763_16763_B2G6F_FP-N_F1_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNCNNNN NNNNNNCNTG NAGCAGCACG ACTTCTTCNA GTCCGCCATG    61 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC   121 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC   181 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC   241 AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC   301 CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC   361 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC   421 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG   481 ATCACTCTCG GCATGGACGA GCTGTACAAG TACTCAGATC CAATGAAAAC ATTTAACATT   541 TCTCAACAGG ATCTAGAATT AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG   601 GATAATAAAC ATCATGTGGG AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA   661 GTACATATTG AAGCGTATAT AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT   721 AGTGCAGTTT CGAATGGACA AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT   781 TCTGACGAAG TAGATAGAAG TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG   841 ATTTCAGACT ATGCACNAGA TTGTTTTGTG TTAATAGAAA TGNNTGGNAA GTTAGTCAAA   901 ACTACGANTG ANNAACTCAT TNNNCTCNAA TATANCCGAA ANTNANGNAN NCNNNCNNAC   961 CGCGGGCCCG GGNNNCNNNN GNATCNAGAN NNCTGNTNAN NANTCNGCCN NNNNNNNNTN 1021 NNNNNNGNNT TTNNNNNNNN TNNAAAANNN NNNNNNN //
Primer: EGFP-M_R (Pr0743) Region: EGFP 5',T2A,SV40 NLS,Cas9 3' Sequence file: RDB16763_A8Kkk.seq
>D05970A1_A8Kk_2_EGFP-M_R_F08_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNNGGNNC TTGNNGTTCN CCTTGNNGCC GTTCTTCTGC    61 TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG   121 ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG   181 CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG   241 CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG   301 TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG GGTGGGCCAG   361 GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC GTAGGTGGCA   421 TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG   481 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGTGGCGACC   541 GGTATCGATC GTGGGCCAGG ATTCTCCTCG ACGTCACCGC ATGTTAGCAG ACTTCCTCTG   601 CCCTCTCCAC TGCCGTTAAC CACTTTGCGC TTTTTCTTGG GGTCGCCTCC CAGCTGAGAC   661 AGGTCGATCC GTGTCTCGTA CAGGCCGGTG ATGCTCTGGT GGATCAGGGT GGCGTCCAGC   721 ACCTCTTTGG TGCTGGTGTA CCTCTTCCGG TCGATGGTGG TGTCNAAGTA CTTGAANGCG   781 GCAGGGGCTC NCAGGTTGGT CAGGGTAAAC ANGTGGATGA TATTCTCGGN CTGCTCTCTG   841 ATGGGCTTAT CCCNGGNGCT TGTTGTANGC GCTCANCACC TTGTCNNGGT TGGCATCGGC   901 CANGATCNNG CGCTTGGANA NNTCNCTAAT CTGNTCNATG ATNTCGTNNN GNNNGNGCTT   961 GTGCTGNTNC NCNAACAGCT GTTTCNGCTN ATTATNCNNN GGGGGANNCN NTNNNNCTNN 1021 NCNNNNNNGN NTNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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