Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAG-Arl13bCerulean-Fucci2a

Mammalian expression construct of Arl13bCerulean-Fucci2a

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. 6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. 6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16059_A9K8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 (Pr0330) Region: CAG pro, insert 5' (Arl13b 5') Sequence file: RDB16059_A9K8a.seq
>D05769A4_A9K8_1_pCAG-F_V2_C05_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTG NGCGGNNCCG ANTCTGGGAN GNGCCGCCGC ACCCCCTCTA    61 GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG GAGGGCCTTC   121 GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT CCGCGGGGGG   181 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG   241 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA   301 ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAC ACGCGTGCTA   361 GCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTATGTTCA GTCTGATGGC CAACTGCTGC   421 AACTTGTTCA AGCGGTGGCG GGAGCCTGTC AGAAAGGTGA CACTTGTGAT GGTCGGCCTT   481 GATAATGCTG GTAAAACAGC CACAGCAAAG GGAATCCAAG GAGAGCATCC TGAANACGTA   541 GCTCCCACTG TTGGCTTTTC TAAAATTGAT CTGAGACAAG GAAAGTTCCA AGTTACCATC   601 TTTGACTTAN GAGGTGGAAA AAGAATTCGG GGAATTTGGA AGAATTATTA TGCTGAATCC   661 TATGGGGTAA TATTTGTTGT GGATTCCAGT GATGANGAGA GAATGGAAGA AACNAAGGAG   721 ACAATGTCCG AANTGCTNAG ACACCCGANG ATATCANGAA AGCCTATATT GGTGCTGGNC   781 AACAAGCAAG ACNAGGNNGG GGNTTTAGGC GAANCGGANG TGATTGANTG NCTCTCGCTG   841 GANAANCTGG NCNACGAGCA CAANNGNCTG TGTCNNATCN AANCTTGTTC ANCNNTCTTG   901 GGANATGGAA ANAAAANTGA NNAGNNNNTN NAAAAGGGGC TTTNNTGGNN NNCTNNNNNT 961 CNTNGNNANN NNNTTTNNNN NCNTNNANNN NNNNCNTCCN NAAAGANNNN NNNNNN //
Primer: mCerulean-N_R (Pr0770) Region: insert mid (mCerulean, Arl13b 3') Sequence file: RDB16059_A9K8b.seq
>D05769A4_A9K8_1_mCerulean-N_R_A06_03_ABI24.ab1     1 NNNNNNNNNN NNGNNNNNNN NNNNGGNNNC TNGCCGGNGG NGCANATGAN CTTCAGGGTC    61 AGCTTGCCGT ANGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT   121 ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG   181 CTCACCATGG TGGCGACCGG TGGATCCCCT GAGATCGTGT CCTGAGCATC ACCGTTAGGT   241 CTCTGTCGCA CAGCCAGGGG AGGCAGCGGC TTCCCATAGA AATCATTGTG ACGTGTTTCA   301 CCAAGAGGCT CAAGTTTTGG AAGTCTAGTG ACTTTGGGGG TTCCCCAGCC AACAGGAGGG   361 GGAGGTTGGG GAGGCGTGGG ACTCTTTGGA GTAGACTCGT CTGTATTGAC TGGTTCTACC   421 CGATGACTCC TTTTCATTCT TAGTTTCTTG GTTTTCTTCT TACTGTTTTC CCCGGACTCT   481 GACCCTCGCT GGTCCTGCTC ATCCTCACTG TCCAGCTGCT GCGACAGTGC CTCCCTGTAA   541 CTGTCCACCA CTCGCTCGTC AGGGTTTTGG AGGCAGCAAT CTGCCTCACT CTGAGGCGCT   601 GCTTGCTCTG GCTCCACTTT ATGTTCTAGG ANANCAACAT CACTGTCCTT CTCCACGGTC   661 TGCTTCTTTT TCTCTTTTTC CTGCTTCTTT TCATTCTCAA TGATTACAGC TGCAATGGGT   721 TGGANAGGAN TCGCAAGAAC TGGTCCTGAN TCAATCTCAG CCAGACCACN NGTCCCATCA   781 AGTNNCGTCT GCTCTCGTTC TCTTTCTTCC CTTANNNTCC NGANTCGTTC ANCCCNCTCA   841 CGTTTCTCTN GTNCCNCNNG ANCTCGCNGT TCANTTNNGN NNTTNNGGAT NCNNNCANTT 901 NNNNNATCNA NNNNCNTNNN NNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), insert 3' (Gem1_1-110, mVenus) Sequence file: RDB16059_A9K8c.seq
>D05769A4_A9K8_1_BGH_rev2_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN GGGGNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGAG CTCTAGATCA TCGAAGGCGC GCCACATGTG GTACCTTACA   121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA   181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG   241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT   301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT   361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT   421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCCT   481 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA   541 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT   601 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA   661 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT   721 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC   781 CCANGATGTT GCCGTCCTCC TTGAANTCGA TGCCCTTCAG CTCGATGCGG TTCANCAGGG   841 NGTCGCCCNC GAACTTCACC TCNGCGCGGG NNTTGTANTT GCCGNCNNCC TTNNNGAANA 901 TGGNGCNCTC CNNGNNNNNN NNNN //
Primer: P2A_R2 (Pr0794) Region: insert mid (mCerulean) Sequence file: RDB16059_A9K8d.seq
>D05769A4_A9K8_1_P2A_R2_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTN NGTAGCTCCG CTTCCCTTGT ACNGCTCGTC CNTGCCGAGA    61 GTGATCCCGN NNNNNNNNNN NAACTCCAGC AGGACCATGT GATCGCGCTT CTCGNTGGNG   121 TCTTTGCTCA GCTTGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG   181 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG   241 TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC GGTGATATAG   301 ACGTTGTCGC TGATGGCGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG   361 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG   421 GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG GACGTAGCCT   481 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGGCGAAG   541 CACTGCACGC CCCAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG   601 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG CATCGCCCTC GCCCTCGCCG   661 GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC   721 CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGNCGA NCGGTGGATC CCCTGAGATC   781 GTGTCCTGAG CATCACCGTT AGGTCTCTGT CGCACAGCCA GGGGNNGCAG CGGCTTCCCA   841 TAGAAATCAT TGTGACGTGT TTCACCAAGA GGCTCAAGTT TTGGAAGTCT ANTGANTTTG   901 GGGGGTTCCC NAGCCAACAG GNNGGGGGAN GTNGGGGANN NGNGGGNNTC TTTGGANTAN   961 ANTCGNCNGT ANTGANNNGN NNCNNCCCNA TNNNTNCTTT TCNTTCNTNN NTTNNNNNNN 1021 TTNNNNNNNN NN //
Primer: mCherry-C_R2 (Pr0792) Region: insert mid (mCherry, P2A, mCerulean) Sequence file: RDB16059_A9K8e.seq
>D05769A4_A9K8_1_mCherry-C_R2_D05_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT TNNGCTTNNG CCTCTGCTTG ATCTCGCCCT TCNGGGCGCC    61 GTCCTCGGGG TACATCCGCT CGGAGGAGGC CTCCCAGCCC ATGGTCTTCT TCTGCATTAC   121 GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC TTGTAGATGA ACTCGCCGTC   181 CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG TCCTCGAAGT TCATCACGCG   241 CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG TCGGGGATGT CGGCGGGGTG   301 CTTCACGTAG GCCTTGGAGC CGTACATGAA CTGAGGGGAC AGGATGTCCC AGGCGAAGGG   361 CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC   421 CTCGCCCTCG CCCTCGATCT CGAACTCGTG GCCGTTCACG GAGCCCTCCA TGTGCACCTT   481 GAAGCGCATG AACTCCTTGA TGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATACG   541 CGCAGGTCCA GGGTTCTCCT CCACGTCTCC AGCCTGCTTC AGCAGGCTGA AGTTAGTAGC   601 TCCGCTTCCC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC   661 CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGGGTGCT   721 CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA   781 GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTGGNCTT   841 GATGCCGTTC TTCTGCTTGN CGGCGGTGAT ATAGACGTTG TCGCTGATGG CGTTGTANTC   901 CNGCTNGTGN CCCNNNATGT TGCCGNCCNN CTTGAAGTCN ANGCCCTNCA NNNCGATGCN 961 GNNNCACNNG GGNNNCNNCN NNNAACTTCN NNNNNGCNNN GNNNNNNNAN NNNNNNNNN //
Primer: mCherry-M_F1 (Pr0767) Region: insert mid (mCherry, Cdt1_30-120, T2A, mVenus) Sequence file: RDB16059_A9K8f.seq
>D05769A4_A9K8_1_mCherry-M_F1_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNA TGNNNAANNN NNNNTGGGCT GGGNNGCCTC CTCCGAGCGG    61 ATGTACCCCG AGGNNGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC   121 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG   181 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC   241 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG   301 TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA GCCCCGCCAG GCCCGCACTC   361 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC   421 GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC GGCTGTCGGT GGACGAGGTT   481 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG   541 ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC TGACATCCGC GCAGGACCAG   601 GACACCATCC AATTGGAAGG CCGTGGCTCT CTTCTCACTT GTGGCGATGT CGAAGAGAAT   661 CCTGGACCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC   721 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGANGGCGAT   781 GCCACCTACN GCAAGCTGAN CCTNAANCTG ATCTGCACCA CCGGCAAGCT GCCCGNGCCC   841 TGGCCCACCC TCGNGACCAC CCTGGGCTAC NGNCTGCAGN GNTTCNCCCG CNANCCCNAC   901 CACNTGAAGC ANCACGACTN CTTCAAGTNC NNCNNGNCCN NNNNNNCNNN CAGNANCGCA   961 NCATCTNCTN CNNNNANGAN NNNNNNTANN NNANCCNNNN CNANNNNNNN NTNNNNNGNN 1021 NNNNNCNNNN NNNNANNNNN NNNNN //
Primer: Cdt1_F1 (Pr0768) Region: insert mid (T2A, mVenus) Sequence file: RDB16059_A9K8g.seq
>D05769A4_A9K8_1_Cdt1_F1_F06_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNGG NNNNCNTCCN ATTGGAAGGC CGTGGCTCTC    61 TTCTCNCTTG TGGCGATGTC GAAGAGAATC CTGGACCCAT GGTGAGCAAG GGCGAGGAGC   121 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT   181 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA   241 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG   301 GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG   361 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA   421 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG   481 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA   541 GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA   601 TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC   661 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCAAGC   721 TGANCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGANCGCCG   781 CCGGGNTCAC TCTCGGCATG NNCGANCTGT ACAANGNNTA TCCATCACAC TGGCGGCCGC   841 TCNANATGAA TCCNANTATG AAGCANAANN NAAGAAGAAA TCAAAGANNN TATAAAGNNN 901 ANTTCNGNCC NNANNANANN TCNNNANNAN NNNTNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl