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pET-LnBiT

Bacterial expression vector of LnBiT, a comporntnt of the Open Sandwich Bioluminescent Immunoassay (OS-BLIA)

Catalog number RDB16003
Resource name pET-LnBiT
Clone info. Bacterial expression vector of LnBiT, a comporntnt of the Open Sandwich Bioluminescent Immunoassay (OS-BLIA), for reconstituted Nanoluc-based ternary protein-fragment complementation assay (PCA) system. For constitution of the assay system, please refer Fig.2 of Ohmuro-Matsuyama and Ueda, Anal. Chem., 90 (5): 3001-3004, 2018. Codons are optimized for E. coli expression.
Comment Expression was confirmed by the depositor with open sandwich immunoassay, SDS-PAGE.
Vector backbone pET32b (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Oplophorus gracilorostris Luciferase cDNA
Depositor|Developer Ueda, Hiroshi | Ohmuro, Yuki |
Other clones in our bank
External Database
Oplophorus gracilorostris Luciferase
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Anal. Chem. 90(5): 3001-3004, 2018). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Anal. Chem. 90(5): 3001-3004, 2018)。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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            RDB16003 pET-LnBiT Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pET-LnBiT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16003).

            Reference section:

            Ohmuro-Matsuyama, Y., Ueda, H., Homogeneous Noncompetitive Luminescent Immunodetection of Small Molecules by Ternary Protein Fragment Complementation. Anal. Chem. 90 (5): 3001-3004 (2018). PMID 29446920.

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Ohmuro-Matsuyama, Y., Homogeneous Noncompetitive Luminescent Immunodetection of Small Molecules by Ternary Protein Fragment Complementation. Anal. Chem. 90 (5): 3001-3004 (2018). PMID 29446920.
            review Ohmuro-Matsuyama, Y., Protein-Protein Interaction Assays Using Split-NanoLuc. Bioluminescence (ed. H. Suzuki) (IntechOpen, London) (2019).

            2023.05.22

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