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pMRX-puro-EGFP-Rab7

Retroviral expression vector of mouse Rab7.

Catalog number RDB15975
Resource name pMRX-puro-EGFP-Rab7
Clone info. Retroviral expression vector of mouse Rab7, fused with EGFP at N-terminus.
Comment Refer AB232599 for complete cds of the mouse Rab7, complete cds,
Vector backbone pMRX-puro-EGFP (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Rab7 cDNA
Reference of insert sequence AB232599 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Rab7
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuchitsu, Y. et al. (2018) J. Cell Sci. in press)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuchitsu, Y. et al. (2018) J. Cell Sci. in press)。
          付加的提供条件：
          pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15975 pMRX-puro-EGFP-Rab7 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Kuchitsu, Y., Rab7 knockout unveils regulated autolysosome maturation induced by glutamine starvation. J. Cell Sci. 131 (7). pii: jcs215442 (2018). PMID 29514857.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Kuchitsu, Y., Rab7 knockout unveils regulated autolysosome maturation induced by glutamine starvation. J. Cell Sci. 131 (7). pii: jcs215442 (2018). PMID 29514857.
          reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2023.04.06

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