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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS423

Bifidobacterium longum–E. coli shuttle vector.

Catalog number RDB15974
Resource name pBS423
Clone info. Bifidobacterium longum–E. coli shuttle vector.
Comment The SpR gene confers spectinomycin resistance both in E. coli and Bifidobacterium longum 105-A. The depositor uses spectinomycin at a concentration of 75 microgram/mL for selection of transformants in agar media. B. longum 105-A (JCM 31944) is available from JCM as a host strain.
Vector backbone pBS423 (Plasmid)
Size of vector backbone 4.9 kb
Selectable markers SpR
Reference of insert sequence AB687999 (DDBJ accession number)
Depositor|Developer Fukiya, Satoru | Kano, Yasunobu |
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External Database

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは,非営利の学術研究に限って提供する。
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            RDB15974 pBS423 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pBS423 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15974).

            Reference section:

            Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15974_A8Fbp1-4.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pBS423_seq3_4 (Pr1260)
            Region: insert mid(repA 5')
            Sequence file: RDB15974_A8Fbg.seq check
            >15974_15974_B2LdF_M2_pBS423_seq3_4_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCGNNNCTTC GCTGGGCTGA NCCATCTGNC TTGGTTCGGC
               61 GCACGCTTGT GCAATGCACA AATGTGCAAT AGGATGTGAG CTATGAGCAA CGAATATGTG
              121 CAGGGGACGC TGGAGCTGAC GCGGGCGTTC GACGGCTGGT GGCTTCCCCA GCGTCCCCTG
              181 TGCTGCGACG ATGACTACTC CCAGCTGGTG CGCCGGAGCC GCATCGACGC GCTCAGATGC
              241 AAGCACATCG AGGCGAATCC GGCAGCGCAG GTGAACATGC TCGTTGTTGA CGTTGATTCG
              301 TCTGAGGGCC GTTTAATGAG CCTGTGGGGG CATCGGGACA TGCCGCCCAA CTTCATCGCG
              361 GAGAATCCGG CCAACGGCCA CTGCCACGCG GGCTGGGTGC TCACCGAACC CGTCTGCCGC
              421 ACCGACATGG CCAGACTCAA GCCCCTGAAG CTCCTGCACG CCGTCACCGA GGGGCTGCGC
              481 CGCAGCGTGG ACGGCGACGA GGGCTATTCC GGCCTGCTCA TGAAGAACCC GTTGAGCGAC
              541 GCGTGGGACA GCGACCTGTG CCGCGAGGAC ACCTACGACC TGCCCGACCT CGTGGCCGCG
              601 CTCGAAGCGC ACGGGGACAT GCCGCCCAAG AGCTGGACGC GCACCAAACG CGCCCGCGAG
              661 GTCGGAGTGG GCCGCAACTG CACCCTGTTC GACGAGGCCC GCACCCTCGC CTACCGCGAG
              721 GTGCGCCGAC TGCCTGACCG CACGCCCGCC TCCTCCGACC TGCTGCGCGA GTACGTGCGC
              781 CGCACCTGCC ACGAAATCAA CGCCTCGTTC CCCGACCCGC TGCCCGTGCG CGAGGTCAAC
              841 GACACCGCCN AGAGCATCCA CAAGTGGATC ACCACNNNAA AGCCGCATGT GGCGANGACG
              901 GTGCCGTCNC CNANCNNCCG CCNNCNTTCG TCNNCNNNCC AATNCCGNNN NNNCNGNAAA
              961 GAAANNNNGG GNGNNGNCCA GANNAAAANN NNNNTTTNNA NNNNNNNNTT CGCGNNNNTN
            1021 ANNNNGCNNN GGANNGNNGC NTNNGNANNN TNGNNCNNNN NNNNNN
            //
            Primer: pTBR101CM_seq3 (Pr0560)
            Region: insert mid(pTB4_p1 3')
            Sequence file: RDB15974_A8Fbh.seq check
            >D04829A3-12_A8Fb_pTBR101CM_seq3_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTGG NNGNTCCGCC GNNCNTGGGG CATGGGTCTT
               61 CCCGGCAAGC CGATTCGCAC CGCGAATCGA GTGATTGTAG CTACAATCAC GTCGCTTGCT
              121 ACTCCGGCCT TCGGCACTCG GGTTGTTGCT CCTGCGCCGA AGGGCGAGGG AGGCTGCGGG
              181 CTCGGTTCAT GTACATGAAC CGTCTAGCTT GCTTACCTTC GATTTGATGG ACATCTCCAT
              241 GTGGATGTCC ATGGAGATGT CCATGGAGAT GTCCATGGAG ATGTCCATGG AGATGTCCAT
              301 GGAGATGTCC ATGTGGGTGT CCATGTGGGT GTCCATGGAT ATATCCATCT GAGCGCATAG
              361 GAAAACGCAT GANGNNGGAT GATCGTTCGG GGTGATGCCT TCTTCCACGA ACGGGAAGGG
              421 GCTCTTCCTT TTTCTGGAAT GTCATCTTCC AGCGCCCTTC GCCTTGAGCT CCTTGAGCAG
              481 CATCGTCGCG TTGTCGCGGA TGCTCGCGTC CGTGAGCGGG GAGAGCTCGT CCGCATAGGA
              541 GCGGCAGAGG AGCGCGTACA CCCGTTCCCG CACCCAGGCG CTGCGCGCGA TGCCCAGCTT
              601 GAACGCGGCC CGGCACAGCA TCTCGTATTC GTCGCCGGTG AGCAGGACAT TGAGCTGGCG
              661 TCAAAAGTTG ATTAGTCATG CCGTGTATAT ACGTCCGGGG TGTTTTCGGC CAGATTCCAA
              721 CAGTTTTGGG CCACTCCGGG AAGGAACAGC CGACGCTTCG CTGGGCTGAA CCATCTGACT
              781 TGGTTCGGCG CACGCTTGTG CAATGCACAA ATGTGCAATA GGATGTGAGC TATGANCAAC
              841 GAATATGTGC ANGGGGANGC TGGANCTGAC GCNGGNGTTC GACGGNNGGN GGCTTCCCCA
              901 GCGNCCCCNG NGCNGCNANG ATGACTACTC CNANCNGGNG NNNCNNNNCC GCATCGNCGC
            961 GCTCNNATGN NNGCACATCN NGGCGANNNC NNNNNNCGCA NNNNNANNNN NNNNNN
            //
            Primer: ColE1ori_F (Pr0393)
            Region: ori,insert(pTB4_p3,pTB4_p2)
            Sequence file: RDB15974_A8Fbi.seq check
            >D04829A3-12_A8Fb_ColE1ori_F_H01_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNC NNCCTCTGNN TTGNNCGTCG ATTTTTGTGA NGCTCGTCAG
               61 GGGGGCGGAN CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTCC CTGGCCTTTT
              121 GCTGGCCTTT TGCTCAGAAT TCGAGCTCGG TACCCGGGGN TCCAGGTGCT TGACAGGGTG
              181 ATGGAGCGTC ACCCAGAACT GTCCGAGAAG GACGTGGTGA CCGCGTTCCG CAGCGTCATG
              241 GTGGACGCGG AGCGCGAGTC CGGCGCGTGG ATGGCGATAG GGCTCGACGG GCGCGGCAGG
              301 AACGTCGAGA TGCTGTACAG GGTCGTCGGC GACCTCGTGG TGATCTACCA CGCGTTCACG
              361 CCGCCGACCA AGAAGTTCAG GCGCGAGATA GACCGTCTGC GCGGCGATGG GAGGACGCTA
              421 TGAGTGATTT CAGCGAACGC GACAGGCAGT TGATGGCGCT GGTCGGGCTG ACCGAGGAGC
              481 AGGTGCTGGA GGATGAGCGG ATGGCCGAGT CCGAGACTGT CCCGGACGAC CTGACGGGGC
              541 GCGTGTACTA CGGGCTCCAT CTCGCCGAGC CCGACGAGCA GATGGTCAGC GTGTCCGTCC
              601 GCATGCCCAA GAGCACGCTG GACGGTCTGA CCGCGATGGC CAGGCGGTAC CACATCAGCC
              661 GCAGCGAGTA CATGCGCCGT AAGCTCGCTG GAGCGATCTG AGACCTGTCA GGCCGATGGT
              721 TCGAATCACG TAACACCCGC GCACCACCGT GAGGATGTGC GGGTGTTACG TGATGTGCCA
              781 CAGTCGTGGC ACGATGCCGC GTGANCGGTC TGGNNGNNTC TGCCGACTAT GGGGCATGGG
              841 TCTTNCCNGC AAGCCGATTC NNACCGNNNA ATCGANNGNA NCGTATATNN NGTTCACGTC
              901 NCTTGCNACN NCNNGNNCNT NGCNGNCTTT TNNCTCNNNN NNCNNNNTNN NNTNGNNGNC
            961 NNNGGTANGG NNCGNTNGGC NNNNNNNNNN
            //
            Primer: pBS423_seq3_5 (Pr1261)
            Region: insert mid(repB 5',repA 3')
            Sequence file: RDB15974_A8Fbj.seq check
            >15974_15974_B2LdF_M2_pBS423_seq3_5_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC GTTNCTTGCG GGCGCGGTAN GCGCGTTGGC
               61 GCACGGCACC TTGGGTCATG TTGAAGTGTT CGGCGGTCTG GCTCCACGAG TGGCCGTCAT
              121 CGTCGTGGTA GGCGCGGATG GATTCGCGCA ACGTTGCCTG CTCGTCTATC CAGTCCTCGC
              181 GCGTTTGCGA ATTCCAGTAC TTGATCGTGC GCACGGACAC CCCATAGGCC TCGGCTAACT
              241 CCTTGGCGGG CAATGGGCGC TTCTTGCGAA TTGTCTGGAT GGTCATTGTC CAAGCACCTC
              301 CTGCGCGTAC TGCGCGAACT TCTCTTCAAA TGCGTTTTGT CTGGCCTCGC CCGATTTCTT
              361 TCCGCGTGCG GATTGGATGG CGACGAACGT GGCGGCGTTG GCGACGGCAC CGTCTCGCCA
              421 CATGCGGCTT CGCGTGGTGA TCCACTTGTG GATGCTCTTG GCGGTGTCGT TGACCTCGCG
              481 CACGGGCAGC GGGTCGGGGA ACGAGGCGTT GATTTCGTGG CAGGTGCGGC GCACGTACTC
              541 GCGCAGCAGG TCGGAGGAGG CGGGCGTGCG GTCAGGCAGT CGGCGCACCT CGCGGTAGGC
              601 GAGGGTGCGG GCCTCGTCGA ACAGGGTGCA GTTGCGGCCC ACTCCGACCT CGCGGGCGCG
              661 TTTGGTGCGC GTCCAGCTCT TGGGCGGCAT GTCCCCGTGC GCTTCGAGCG CGGCCACGAN
              721 GTCGGGCAGG TCGTANGTGT CCTCNCGGCN NANGTCGCTG TNNCACNCNN CNNTNNNNGG
              781 GNNTNCTTCA TGANCAGGCC GGAATAGCCC TCGNCNCNNN NNNNNNCTNN CNGNNGCANN
              841 NCCNNNCNGG TGANNGCNTG CNNNNNCTTC NNNGGGCTTN NANNNNGNNN NNNNNCNGTG
              901 NNGNNNNAAN NGGTTTCNGG TGANCNNNNC ANNCCNNNNG NNANNNNGNN NNTNGGCCNN
            961 NNNNNNNCNN NNNNNNNN
            //
            Primer: pTBR101CM_seq7 (Pr0561)
            Region: insert mid(repB, 3',pTB4_p2,pTB4_p3 3')
            Sequence file: RDB15974_A8Fbk.seq check
            >D04829A3-12_A8Fb_pTBR101CM_seq7_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNTCCGC GCCNANNNCG ACNATGNNGG CCACTCGTGG ANCCAGACCG
               61 CCGAACACTT CAACATGACC CAAGGTGCCG TGCGCCAACG CGCCTACCGC GCCCGCAAGG
              121 AACGCGAGGC CGAGGCAAAA GCCGCAAGGC CGGAGTAGCA AGTGACGTGA ACGTATATAC
              181 GTTCACTCGA TTCGCGGTGC GAATCGGCTT GCCGGGAAGA CCCATGCCCC ATAGTCGGCA
              241 GAGCCTCCAG ACCGCTCACG CGGCATCGTG CCACGACTGT GGCACATCAC GTAACACCCG
              301 CACATCCTCA CGGTGGTGCG CGGGTGTTAC GTGATTCGAA CCATCGGCCT GACAGGTCTC
              361 AGATCGCTCC AGCGAGCTTA CGGCGCATGT ACTCGCTGCG GCTGATGTGG TACCGCCTGG
              421 CCATCGCGGT CAGACCGTCC AGCGTGCTCT TGGGCATGCG GACGGACACG CTGACCATCT
              481 GCTCGTCGGG CTCGGCGAGA TGGAGCCCGT AGTACACGCG CCCCGTCAGG TCGTCCGGGA
              541 CAGTCTCGGA CTCGGCCATC CGCTCATCCT CCAGCACCTG CTCCTCGGTC AGCCCGACCA
              601 GCGCCATCAA CTGCCTGTCG CGTTCGCTGA AATCACTCAT AGCGTCCTCC CATCGCCGCG
              661 CAGACGGTCT ATCTCGCGCC TGAACTTCTT GGTCGGCGGC GTGAACGCGT GGTAGATCAC
              721 CACGAGGTCG CCGACGACCC TGTACAGCAT CTCGACGTTC CTGCCGCGCC CGTCGAGCCC
              781 TATCGCCATC CACGCGCCGG ANTCGCGCTC CGCGTCCACC ATGACGCTGC GGAACGCGGT
              841 CACCACGTCC TTCTCNGANA GTTCTGGGTG ANGCTCCATC ACCCNGNCAA GCACCTGGNT
              901 NCCCGGGNAC CGANCTCNAA TTCNGANCAA AANGNNNGCA AAAGGNNNNG GNNNNTAAAA
              961 NNNNCNNGTN GCNNGNNNNT TTTNCNNNNN NNCNNNCCCC NNNNNNANCN NNNNNAANNN
            1021 NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061.
            reference Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016).

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl