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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSilencer-neo-Rab5A

Expression vector of shRNA against mouse Rab5A.

Catalog number RDB15727
Resource name pSilencer-neo-Rab5A
Clone info. Expression vector of shRNA against mouse Rab5A (19-base target site 5'-GCACAGTCCTATGCAGATG-3'). U6 promoter.
Comment Expression was confirmed by the depositor with confocal microscopy. Please refer Mori, Y. et al., J. Biol. Chem., 288 (14): 9835-9847, 2013 (PMID 23430262) for gene cloning.
Vector backbone pSilencer 2.1-U6 neo (plasmid)
Size of vector backbone 4.5 kb
Selectable markers Amp^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab5a cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab5a

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mori, Y. et al. J. Biol. Chem. 288, 9835-9847, 2013); (Azouz, N. P. et al. J. Immunol. 192, 4043-4053, 2014)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. J. Biol. Chem. 288, 9835-9847, 2013); (Azouz, N. P. et al. J. Immunol. 192, 4043-4053, 2014)。
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          RDB15727 pSilencer-neo-Rab5A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Azouz, N.P., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15727_A8A9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13fwd
          Sequence file: RDB15727_A8A9a.seq check
          >D05154A4_1_A8A9_M13fwd_A02_02_ABI24.ab1
              1 CGGACGGCGC AGGCAACGCA CCACGTGACG GAGCGTGACC GCGCGCCGAG CGCGCGCCAA
             61 GGTCGGGCAG GAAGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA
            121 GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA
            181 TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA
            241 AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG GTTTATATAT
            301 CTTGTGGAAA GGACGCGGGA TCCGCACAGT CCTATGCAGA TGTTCAAGAG ACATCTGCAT
            361 AGGACTGTGC TTTTTTGGAA AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
            421 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC
            481 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
            541 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
            601 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT
            661 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
            721 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
            781 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
            841 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
            901 CCTGGAAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GGATACCTGT
            961 CCGCCTTTCT CCCTTTCGGA GCGTGGCGCT TTTCTCATAG CTCACGGCTG TAGTATCTCA
           1021 GTTCGGTGTA AGTTCGGTCG CTCTAGCTGG CTGTGTGCAC GACCCCCCGA TCACCCGACG
          1081 CTGCGCTTAT CAGTACCTAT CGATCCTTGG AGATC
          //
          Primer: SV40pro_F
          Sequence file: RDB15727_A8A9b.seq check
          >D05154A4_1_A8A9_SV40pro_F_C02_08_ABI24.ab1
              1 CCTTGGGTTC CCGGGCATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
             61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG
            121 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
            181 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
            241 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
            301 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
            361 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
            421 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
            481 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
            541 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
            601 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC
            661 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
            721 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
            781 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
            841 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCCTTC
            901 TATCGCCTTT CTTGACGAGT TCTTTCTGAG CGGGACTCTG GGGGTTCGCG AAATGACCGA
            961 CCAAGCGACG GCCCAACCTG CCATCACGAG ATTTCGATTT CAACGCGCCT TTCTATGAAA
           1021 GGTTGGGGCT CGGAATCGTT TCCGGACGTG GCTGGATGAT CTTCAGCGCG GGGAATCTCA
          1081 TGCTTGGAGT CCTTTTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Azouz, N.P., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234.
          reference Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl