Please review the QC test results indicated by icon below as well as clone information before placing your order.

pENTER-EGFR L858R

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-activating mutation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Uchibori, K et al. Nat. Commun. 8: 14768, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15493_A7K8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F2 (Pr0531) Region: rrnB T1 ter,attL1,insert 5' Sequence file: RDB15493_A7K8d.seq
>D04925A6_A7K8_2_rrnBT1ter_F2_F04_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCCTTTCGTT TTNTTTGATG CCTGGCAGTT CCCTACTCTC    61 GCGTTAACGC TAGCATGGAT GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTCTT   121 AAGCTCGGGC CCCAAATAAT GATTTTATTT TGACTGATAG TGACCTGTTC GTTGCAACAA   181 ATTGATGAGC AATGCTTTTT TATAATGCCA ACTTTGTACA AAAAAGCAGG CTCCGCGGCC   241 GCCCCCTTCA CCATGCGACC CTCCGGGACG GCCGGGGCAG CGCTCCTGGC GCTGCTGGCT   301 GCGCTCTGCC CGGCGAGTCG GGCTCTGGAG GAAAAGAAAG TTTGCCAAGG CACGAGTAAC   361 AAGCTCACGC AGTTGGGCAC TTTTGAAGAT CATTTTCTCA GCCTCCAGAG GATGTTCAAT   421 AACTGTGAGG TGGTCCTTGG GAATTTGGAA ATTANNNATG TGCAGAGGAA TTATGATCTT   481 TCCTTCTTAA AGACCATCCA GGAGGTGGCT GGTTATGTCC TCATTGCCCT CAACACAGTG   541 GAGCGAATTC CTTTGGAAAA CCTGCAGATC ATCAGAGGAA ATATGTACTA CGAAAATTCC   601 TATGCCTTAG CAGTCTTATC TAACTATGAT GCAAATAAAA CCGGACTGAA GGAGCTGCCC   661 ATGAGAAATT TACAGGAAAT CCTGCATGGC GCCGTGCGGT TCAGCAACAA CCCTGCCCTG   721 TGCAATGTGG AGAGCATCCA GTGGCGGGAC ATAGTCAGCA GTGACTTTCT CAGCAACATG   781 TCGATGGACT TCCAGAACCA CCTGGGCAGC TGCCAAAAGT GTGATCCAAG CTGTCNNAAN   841 GNNANCTGCT GGGGTGCANG NNANGANAAN TGCCAGAAAC TGANCAAANT CATCTGTGCC   901 CAGCAGTGNN NCNGGNGCTG CCNNNGNAAN NNCCCCCANN NNNNGCNNNC NCAACCANNN 961 NNNTNNNNNN NNNN //
Primer: pDONR_R (Pr0071) Region: attL2,insert 3' Sequence file: RDB15493_A7K8e.seq
>D04925A6_A7K8_2_pDONR_R_H05_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNCNNT GNNNNTGTNA TACGACTCNC TATAGGGGAN ATCAGCTGGA    61 TGGCAAATAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATAAG   121 CAATGCTTTC TTATAATGCC AACTTTGTAC AAGAAAGCTG GGTCGGCGCG CCCACCCTTT   181 CATGCTCCAA TAAATTCACT GCTTTGTGGC GCGACCCTTA GGTATTCTGC ATTTTCAGCT   241 GTGGAGCCCT TAAAGATGCC ATTTGGCTTG GCTTCCTTGG GAAAGAAGTC CTGCTGGTAG   301 TCAGGGTTGT CCAGGCTAAT TTGGTGGCTG CCTTTCTGGG CCCAGTGGGC AGGGCTGTCG   361 AATGTGCTGT TGACACAGGT GGGCTGGACA GTGTTGAGAT ACTCGGGGTT GCCCACTGCA   421 GTGCTGTGGG GGTCCTGGTA GTGTGGGTCT CTGCTGGGCG CGGGGTTCAG AGGCTGATTG   481 TGATAGACAG GATTCTGCAC AGAGCCAGCG GGCCTTTTGG GAACGGACTG GTTTATGTAT   541 TCAGGCACTG GGAGGAAGGT GTCGTCTATG CTGTCCTCAG TCAAGGCGCC TGTGGGGTCT   601 GAGCTGTATC GCTGCAAGAA GCTGTCTTCC TTGATGGGAC AGCTTTGCAG CCCATTTCTA   661 TCAATGCAAG CCACGGTGGA ATTGTTGCTG GTTGCACTCA GAGAGCTCAN GANGGGAGTC   721 CGTGACGTGG ANGGGCTGCT GAANAAGCCC TGCTGTGGGA NGANGTACTC GTCGGCATCC   781 ACCACGTCGT CCATGTCTTC TTCATCCNTC NNGNCACGGT ANAAGTTGGA NTCTGTANGA   841 NTTNNCAAAT GCATTCTTTC NTCCCCCCNG ANTGANAAGG NANCGCTNGG GGGTCNCGGG   901 CCNNTTTNGG ANNANNTCNA TGANCNNNTC NNNGNANNNT TNGGNNNANN ATCTGCNNCT 961 ATNNNNNNNN NN //
Primer: EGFR-F5 (Pr0313) Region: insert mid (L858R) Sequence file: RDB15493_A7K8f.seq
>D04925A6_A7K8_2_EGFR-F5_F09_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGGNNGGT GGGGGCCCTC CTCTTGCTGC TGGTGGTGGC    61 CCTGGGGNNC GGCCTCTTCA TGCGAAGGCG CCACATCGTT CGGAAGCGCA CGCTGCGGAG   121 GCTGCTGCAG GAGAGGGAGC TTGTGGAGCC TCTTACACCC AGTGGAGAAG CTCCCAACCA   181 AGCTCTCTTG AGGATCTTGA AGGAAACTGA ATTCAAAAAG ATCAAAGTGC TGGGCTCCGG   241 TGCGTTCGGC ACGGTGTATA AGGGACTCTG GATCCCAGAA GGTGAGAAAG TTAAAATTCC   301 CGTCGCTATC AAGGAATTAA GAGAAGCAAC ATCTCCGAAA GCCAACAAGG AAATCCTCGA   361 TGAAGCCTAC GTGATGGCCA GCGTGGACAA CCCCCACGTG TGCCGCCTGC TGGGCATCTG   421 CCTCACCTCC ACCGTGCAAC TCATCACGCA GCTCATGCCC TTCGGCTGCC TCCTGGACTA   481 TGTCCGGGAA CACAAAGACA ATATTGGCTC CCAGTACCTG CTCAACTGGT GTGTGCAGAT   541 CGCAAAGGGC ATGAACTACT TGGAGGACCG TCGCTTGGTG CACCGCGACC TGGCAGCCAG   601 GAACGTACTG GTGAAAACAC CGCAGCATGT CAAGATCACA GATTTTGGGC GGGCCAAACT   661 GCTGGGTGCG GAAGAGAAAG AATACCATGC AGAAGGAGGC AAAGTGCCTA TCAAGTGGAT   721 GGCATTGGAA TCAATTTTAC ACAGAATCTA TACCCACCAG AGTGATGTCT GGAGCTACNG   781 GGTGACCGTT TGNGAGTTGA TGANCTTTGG ATCCNAGCCA TATGACGGAA TCCCTGCCAG   841 CGNNNTCTCC TCCATCCTGG ANAAAGGANA ANGCCTCCCT CANNCNCCCA TATNNACCAT   901 CNNTGTCTAC NTNNNCNTGG NCAANNNNTN NNNGNNANNC GNNNNNANTC NCCNNANNNT 961 NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl