Please review the QC test results indicated by icon below as well as clone information before placing your order.

pENTER-EGFR wild-type

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), wild type.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Uchibori, K et al. Nat. Commun. 8: 14768, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15488_A7K8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F2 Region: rrnB T1 ter, attL1, insert 5' Sequence file: RDB15488_A7K8a.seq
>D04925A1_A7K8_2_rrnBT1ter_F2_A04_01_ABI24.ab1     1 AGTCAGATCT TCGACTGAGC CTTTCGTTTT ATTTGATGCC TGGCAGTTCC CTACTCTCGC    61 GTTAACGCTA GCATGGATGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTCTTAA   121 GCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG ACCTGTTCGT TGCAACAAAT   181 TGATGAGCAA TGCTTTTTTA TAATGCCAAC TTTGTACAAA AAAGCAGGCT CCGCGGCCGC   241 CCCCTTCACC ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC   301 GCTCTGCCCG GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA   361 GCTCACGCAG TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA   421 CTGTGAGGTG GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC   481 CTTCTTAAAG ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA   541 GCGAATTCCT TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA   601 TGCCTTAGCA GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT   661 GAGAAATTTA CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG   721 CAATGTGGAG AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC   781 GATGGACTTC CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG   841 GAGCTGCTGG GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA   901 GCAGTGCTCC GGGCGCTGCC GTGGCAAGTC CCCCCAGTGA CTGCTGCCAC AACCAGTGTG   961 CTGCAGGCTG CACAGGCCCC CCGGGAGAGC GACTGCCCTG GGTCTGCCGC AAATTCCCGA  1021 GACGAAAGCC ACGTGCAAGG ACACCTGCCC CCCCCACTCA TGCTCTACAA CCCCCACCAC 1081 GTACCAGATG TATGTGAAAC CCCGAGGGGC AAAATAACAA GCCTTTTTGG AATGC //
Primer: pDONR_R Region: attL2, insert 3' Sequence file: RDB15488_A7K8b.seq
>D04925A1_A7K8_2_pDONR_R_A08_02_ABI24.ab1     1 GGCGGACGTC AGGGACGCTA TGACCATGTA ATACGACTCA CTATAGGGGA TATCAGCTGG    61 ATGGCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATAA   121 GCAATGCTTT CTTATAATGC CAACTTTGTA CAAGAAAGCT GGGTCGGCGC GCCCACCCTT   181 TCATGCTCCA ATAAATTCAC TGCTTTGTGG CGCGACCCTT AGGTATTCTG CATTTTCAGC   241 TGTGGAGCCC TTAAAGATGC CATTTGGCTT GGCTTCCTTG GGAAAGAAGT CCTGCTGGTA   301 GTCAGGGTTG TCCAGGCTAA TTTGGTGGCT GCCTTTCTGG GCCCAGTGGG CAGGGCTGTC   361 GAATGTGCTG TTGACACAGG TGGGCTGGAC AGTGTTGAGA TACTCGGGGT TGCCCACTGC   421 AGTGCTGTGG GGGTCCTGGT AGTGTGGGTC TCTGCTGGGC GCGGGGTTCA GAGGCTGATT   481 GTGATAGACA GGATTCTGCA CAGAGCCAGC GGGCCTTTTG GGAACGGACT GGTTTATGTA   541 TTCAGGCACT GGGAGGAAGG TGTCGTCTAT GCTGTCCTCA GTCAAGGCGC CTGTGGGGTC   601 TGAGCTGTAT CGCTGCAAGA AGCTGTCTTC CTTGATGGGA CAGCTTTGCA GCCCATTTCT   661 ATCAATGCAA GCCACGGTGG AATTGTTGCT GGTTGCACTC AGAGAGCTCA GGAGGGGAGT   721 CCGTGACGTG GAGGGGCTGC TGAAGAAGCC CTGCTGTGGG ATGAGGTACT CGTCGGCATC   781 CACCACGTCG TCCATGTCTT CTTCATCCAT CAGGGCACGG TAGAAGTTGG AGTCTGTAGG   841 ACTTGGCAAA TGCATTCTTT CATCCCCCCT GAATGACAAG GTAGCGCTGG GGGGTCTCGG   901 GGCCATTTTG GAGAATTCGA TGATCAACTC ACGGGAACTT TGGGGCGACT ATCTGCGTCT   961 ATCATCCAGC ACTTGACCAT GATCATGTAG ACATCGATGG TACATATGGG TGGCTGAGGG  1021 AGGCGTTTCT CCCTTTTCTC CAGGATGGAG GAGATCTCGC TGGCAGGATC GGTCATATGG 1081 CCTTGGCATC CAAGGATCAA TCAACTTCTC A //
Primer: EGFR-F5 Region: insert middle region Sequence file: RDB15488_A7K8c.seq
>D04925A1_A7K8_2_EGFR-F5_A09_03_ABI24.ab1     1 AACAATAATC GTCCATCGCC ACTGGGATGG TGGGGGCCCT CCTCTTGCTG CTGGTGGTGG    61 CCCTGGGGAT CGGCCTCTTC ATGCGAAGGC GCCACATCGT TCGGAAGCGC ACGCTGCGGA   121 GGCTGCTGCA GGAGAGGGAG CTTGTGGAGC CTCTTACACC CAGTGGAGAA GCTCCCAACC   181 AAGCTCTCTT GAGGATCTTG AAGGAAACTG AATTCAAAAA GATCAAAGTG CTGGGCTCCG   241 GTGCGTTCGG CACGGTGTAT AAGGGACTCT GGATCCCAGA AGGTGAGAAA GTTAAAATTC   301 CCGTCGCTAT CAAGGAATTA AGAGAAGCAA CATCTCCGAA AGCCAACAAG GAAATCCTCG   361 ATGAAGCCTA CGTGATGGCC AGCGTGGACA ACCCCCACGT GTGCCGCCTG CTGGGCATCT   421 GCCTCACCTC CACCGTGCAA CTCATCACGC AGCTCATGCC CTTCGGCTGC CTCCTGGACT   481 ATGTCCGGGA ACACAAAGAC AATATTGGCT CCCAGTACCT GCTCAACTGG TGTGTGCAGA   541 TCGCAAAGGG CATGAACTAC TTGGAGGACC GTCGCTTGGT GCACCGCGAC CTGGCAGCCA   601 GGAACGTACT GGTGAAAACA CCGCAGCATG TCAAGATCAC AGATTTTGGG CTGGCCAAAC   661 TGCTGGGTGC GGAAGAGAAA GAATACCATG CAGAAGGAGG CAAAGTGCCT ATCAAGTGGA   721 TGGCATTGGA ATCAATTTTA CACAGAATCT ATACCCACCA GAGTGATGTC TGGAGCTACG   781 GGGTGACCGT TTGGGAGTTG ATGACCTTTG GATCCAAGCC ATATGACGGA ATCCCTGCCA   841 GCGAGATCTC CTCCATCCTG GAGAAAGGAG AACGCCTCCC TCAGCCACCC ATATGTACCA   901 TCGATGTCTA CATGATCATG GTCAAGTGCT GGATGATAGA CGCAGATAGT CGCCCAAAGT   961 TCCGTGAGTT GATCATCGAA ATTCTCCAAA ATGGGCCGAG ACCCCCAGCG CTACCCTTGT  1021 CATTCAGGGG GATGAAAGAA TGCATTTGCC CAGTCCTACA GACTCCAACT CTAACGTGTC 1081 TGGATTGATG AAGAGGACAT TGGCACGAAA CCGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl