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YCX2.60/pCS2

Yellow cameleon calcium ion sensor clone YCX2.60.

Catalog number RDB15455
Resource name YCX2.60/pCS2
Clone info. Calcium ion sensor clone YCX2.60 Yellow cameleon having wild-type CaM and the circular permutated cp173Venus. A new version of YC indicator with an expanded dynamic range.
Vector backbone pCS2 (Plasmid)
Selectable markers Amp^r
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC025957 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
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External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
        Madisen, L., Garner, A.R., Shimaoka, D., Chuong, A.S., Klapoetke, N.C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., Gu, H., Mills, M., Cheng, A., Tasic, B., Nguyen, T.N., Sunkin, S.M., Benucci, A., Nagy, A., Miyawaki, A., Helmchen, F., Empson, R.M., Knopfel, T., Boyden, E.S., Reid, R.C., Carandini, M., Zeng, H. (2015) Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron, 85 (5): 942-958.
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        提供条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
        Madisen, L., Garner, A.R., Shimaoka, D., Chuong, A.S., Klapoetke, N.C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., Gu, H., Mills, M., Cheng, A., Tasic, B., Nguyen, T.N., Sunkin, S.M., Benucci, A., Nagy, A., Miyawaki, A., Helmchen, F., Empson, R.M., Knopfel, T., Boyden, E.S., Reid, R.C., Carandini, M., Zeng, H. (2015) Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron, 85 (5): 942-958.
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        提供依頼書 [Word]
        提供同意書 (MTA, 非営利学術目的用)[Word]
        提供同意書 (MTA, 営利目的用)[Word]
        手続きの概要は、「提供申込みについて[link]」をご覧ください。
        備考 提供条件は英文のまま記載してください。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15455 YCX2.60/pCS2 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The YCX2.60/pCS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15455).

        Reference section:

        Madisen, L., Garner, A.R., Shimaoka, D., Chuong, A.S., Klapoetke, N.C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., Gu, H., Mills, M., Cheng, A., Tasic, B., Nguyen, T.N., Sunkin, S.M., Benucci, A., Nagy, A., Miyawaki, A., Helmchen, F., Empson, R.M., Knöpfel, T., Boyden, E.S., Reid, R.C., Carandini, M., Zeng, H., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


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        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15455_A7Jvp1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pCMV-IE94_F
        Sequence file: RDB15455_A7Jva.seq check
        >D05018B6-2_A7Jv_pCMV-IE94_F_G02_20_ABI24.ab1
            1 AGGCGGGGTG GCGTGCCTAA TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAACCGCC
           61 ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA CACTATAGAA TACAAGCTAC
          121 TTGTTCTTTT TGCAGGATCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG
          181 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG
          241 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA
          301 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTGGGGCGTG CAGTGCTTCA
          361 GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT
          421 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG
          481 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG
          541 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CATCAGCCAC AACGTCTATA
          601 TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG
          661 AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC
          721 CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA
          781 ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCCGC ATGCATACAG
          841 AAGAGCAGAT TGCAGAGTTC AAAGAAGCCT TCTCATTATT CGACAAGGAT GGGGGACGGC
          901 ACCATCACCA CAAAGGAACT TGGCACCGTT ATGAGGTCGC TTGGACAAAA CCCAACGGAA
          961 AGCAGAATTG CAGGATATGA TCAATGAAAG TCGATGCTGA TGGGCGACGG AACGATTTAC
         1021 TTTTCCCTGA ATTTCTTACT ATGATGGGCT AGAAAAATGA AGTTACACAG GACCAGCCGA
        1081 GGAAGGAATC GAGCAGCATC GTGCTTTTTG GACCAGGGAT GGGGAAACGG TCT
        //
        Primer: pAxCALNL_F1
        Sequence file: RDB15455_A7Jvb.seq check
        >D05018B6-2_A7Jv_pAxCALNLF1_H02_23_ABI24.ab1
            1 CCTAATGTTT CTTTAATCAT GTCTGGATCT ACGTAATACG ACTCACTATA GTTCTAGAGG
           61 CTCGAGAGGC CTTGAATTCT TACTCGATGT TGTGGCGGAT CTTGAAGTTG GCCTTGATGC
          121 CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT
          181 TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG ATGCGGTTCA
          241 CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA
          301 AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG AAGTCGTGCT
          361 GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG GTGGTCACGA
          421 GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT CAGCTTCAGG GTCAGCTTGC
          481 CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC
          541 CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA
          601 TGCCACCGCT GCCACCCTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA
          661 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT
          721 GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT
          781 GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CGTCCATGAG CTCCAGTGCC CCGGAGCTGG
          841 AGGACTTCTT GAACCGGTTG GCAGCGCTGA CGGCAATGAA GTTTTTCTTC CAGCGCCTCT
          901 TCCCCCCCCT TTGCTGTCAT CATTTGTACA AACTCTTCAT AGTTTACTTG GGCCATCACC
          961 ATCGATATCT GCCACCCTTA TCATTTCATC AACTTTCTTC ATCTGTTAAC TTCTCCCCGG
         1021 AGGTTTGTCA TGACGTGACG TAAATTTCAG CAGCGGCTGA TGTAGTCGTT TCCCATCCCT
         1081 TTGTCAAAAA CACGGAATTG CATCTCGGAA TTTTCGTCGT TCGGCTTGTC GTGGGTGTAA
        1141 CCC
        //
        Primer: YCX2.6_F1
        Sequence file: RDB15455_A7Jvc.seq check
        >D0518B6_2_A7Jv_YCX2.6_F1_H01_08_ABI08.ab1
            1 AATTAAGGAA GCCTTCTCAT TATTCGACAA GGATGGGGAC GGCACCATCA CCACAAAGGA
           61 ACTTGGCACC GTTATGAGGT CGCTTGGACA AAACCCAACG GAAGCAGAAT TGCAGGATAT
          121 GATCAATGAA GTCGATGCTG ATGGCGACGG AACGATTTAC TTTCCTGAAT TTCTTACTAT
          181 GATGGCTAGA AAAATGAAGT ACACAGACAG CGAAGAGGAA ATCCGAGAAG CATTCCGTGT
          241 TTTTGACAAG GATGGGAACG GCTACATCAG CGCTGCTGAA TTACGTCACG TCATGACAAA
          301 CCTCGGGGAG AAGTTAACAG ATGAAGAAGT TGATGAAATG ATAAGGGTGG CAGATATCGA
          361 TGGTGATGGC CAAGTAAACT ATGAAGAGTT TGTACAAATG ATGACAGCAA AGGGGGGGAA
          421 GAGGCGCTGG AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA AGAAGTCCTC
          481 CAGCTCCGGG GCACTGGAGC TCATGGACGG CGGCGTGCAG CTCGCCGACC ACTACCAGCA
          541 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA
          601 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
          661 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGTGGCA GCGGTGGCAT
          721 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
          781 CGACGTAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
          841 AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
          901 GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG
          961 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGGAGCGCA CCATCTTCTT
         1021 TCAAGGACGA CGGGCACTAC AGACCCGCGC CGAGGGTGAA GTTCGAGGCG ACACCCTTGG
         1081 TGAACCGCAT CGAGCTGAAG GCATCGACTC CAAGAGAACG CAACATCCTG GGCAACAAGC
        1141 TGGGAGTTAC CACCTTTA
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Madisen, L., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722. [link to RRC of NBRP]

        2023.11.23

        GNP_filter3_RDBDEP_html_231027.pl