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tFucci(SA)2/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15443
Resource name tFucci(SA)2/pCSII-EF
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC192886 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions:
        The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering
        Order form [Credit Card Payment]  [Bank Transfer Payment]
        MTA, for use for not-for-profit academic purpose [Word]
        Please visit Ordering instruction.[link] 
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件:
        レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼
        提供依頼書 [Word]
        提供同意書 (MTA, 非営利学術目的用)[Word]
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15443 tFucci(SA)2/pCSII-EF DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The tFucci(SA)2/pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15443).

        Reference section:

        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


        Tips

        Featured content

        Featured content Lentiviral plasmid list (English text)
        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15443_A7Jvp1-3.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: EF1a-C_F (Pr0168)
        Region: EF-1a pro,insert5'
        Sequence file: RDB15443_A7Jva.seq check
        >D05018A3_2_A7Jv_CMV-Forward_H02_08_ABI08.ab1
            1 CTTTAAACCT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
           61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
          121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
          181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
          241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
          301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
          361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
          421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
          481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
          541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
          601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
          661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
          721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
          781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGGACAAT TGGAGAAGTG AATTATATAA
          841 ATATAAAGTA GTAAAAATTG AAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAAGA
          901 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA AATAGGAGCT TTGTTCCCTT GGGTTCTTGG
          961 GAGCAGCAGG AAAGCACTAT GGGGCGCAGC GTCAATGACG CTGACGGGTA CAGGGCCCAG
         1021 ACATTTATTT GTCTGGGTAT AGTTGCAAGC AGCAGACAAA TTTTGCCTGA GGCTATTGAG
         1081 GCGCCACCAG CATCCTGTTG GCAACTTCAC AGTCTGGGGG CAATCCAGGC AAGCCTCCAA
        1141 GGCAGGAAGT CTGTGTGGGC TTGTGTTGGA
        //
        Primer: mCherry-C_F (Pr0453)
        Region: insert(mid)
        Sequence file: RDB15443_A7Jvb.seq check
        >D05018A3_A7Jv_2_BGH_rev2_A02_02_ABI24.ab1
            1 CCAGTTAGGA TCAGAGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA
           61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
          121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
          181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
          241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
          301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
          361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
          421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
          481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
          541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
          601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
          661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
          721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
          781 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG
          841 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA
          901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG
          961 GCGAGCAGCC ATGGAAAAGG ACGTCAGCTT TCCCCGACAC ACCACGGGAA TTGTCAGTGC
         1021 CCACAGCCGA GCCCCTGTCC AGCAGCGGGC AGCAGCGCGA TGAGGTTCGG TCGTGCATAG
         1081 GAGGGGGAAA GCGAAGGTCC GCAAGAGCCT GACAGTGTTG TATGCCTCCT AACTCAGTGA
        1141 G
        //
        Primer: PRE_rev (Pr0250)
        Region: PRE,insert3'
        Sequence file: RDB15443_A7Jvc.seq check
        >D05018A3_A7Jv_2_SV40pro_F_B02_05_ABI24.ab1
            1 ACCTTGTCCT GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
           61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
          121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
          181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
          241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
          301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
          361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
          421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
          481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
          541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
          601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
          661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
          721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
          781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
          841 TAATCATGGT CATAGCTGTT TCCTGGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
          901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
          961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCCAGCTGC
         1021 ATTAATGAAA TCGGGCCAAC GCGCGGGGAG AGCGGGTTTT GCGTATTGGG CGCTCTTCGG
         1081 CTTTCTCGCT CACTGAACTT CGCTTGCGCC TCGATTCGAT CCGGGCCTGG CGGACGAGCG
        1141 GGTAATTCCA GGCCTTCAAC T
        //
        Primer: CMV_Forward (Pr0016)
        Region: CMVpro,5'LTR,HIV-1psi
        Sequence file: RDB15443_A7Jvd.seq check
        >D05018A3_2_A7Jv_CMV-Forward_H02_08_ABI08.ab1
            1 CTTTAAACCT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
           61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
          121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
          181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
          241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
          301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
          361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
          421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
          481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
          541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
          601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
          661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
          721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
          781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGGACAAT TGGAGAAGTG AATTATATAA
          841 ATATAAAGTA GTAAAAATTG AAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAAGA
          901 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA AATAGGAGCT TTGTTCCCTT GGGTTCTTGG
          961 GAGCAGCAGG AAAGCACTAT GGGGCGCAGC GTCAATGACG CTGACGGGTA CAGGGCCCAG
         1021 ACATTTATTT GTCTGGGTAT AGTTGCAAGC AGCAGACAAA TTTTGCCTGA GGCTATTGAG
         1081 GCGCCACCAG CATCCTGTTG GCAACTTCAC AGTCTGGGGG CAATCCAGGC AAGCCTCCAA
        1141 GGCAGGAAGT CTGTGTGGGC TTGTGTTGGA
        //
        Primer: SV40pro_F (Pr0110)
        Region: SV40pro_ori,EM7pro,BleoR,SV40p(A)
        Sequence file: RDB15443_A7Jvg.seq check
        >15443_15443_A7Jv_EF1a-N_R_A02_02_ABI24.ab1
            1 CGGAGGGGCT GTGGGCGATG TGCGCTCTGC CCACTGACGG GCACCGGAGC CTCACGAAGC
           61 TTGAATTAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA AACCCGAAAA TTTTGAATTT
          121 TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT TGCTATTATG TCTACTATTC
          181 TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT AAAATTGTGG ATGAATACTG
          241 CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC TGTCTCTCTC TCCACCTTCT
          301 TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG GGAGGTGGGT CTGAAACGAT
          361 AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA GTACAGCAAA AACTATTCTT
          421 AAACCTACCA AGCCTCCTAC TATCATTATG AATAATTTTA TATACCACAG CCAATTTGTT
          481 ATGTTAAACC AATTCCACAA ACTTGCCCAT TTATCTAATT CCAATAATTC TTGTTCATTC
          541 TTTTCTTGCT GGTTTTGCGA TTCTTCAATT AAGGAGTGTA TTAAGCTTGT GTAATTGTTA
          601 ATTTCTCTGT CCCACTCCAT CCAGGTCGTG TGATTCCAAA TCTGTTCCAG AGATTTATTA
          661 CTCCAACTAG CATTCCAAGG CACAGCAGTG GTGCAAATGA GTTTTCCAGA GCAACCCCAA
          721 ATCCCCAGGA GCTGTTGATC CTTTAGGTAT CTTTCCACAG CCAGGATTCT TGCCTGGAGC
          781 TGCTTGATGC CCCAGACTGT GAGTTGCAAC AGATGCTGTT GCGCCTCAAT AGCCCTCAGC
          841 AAATTGTTCT GCTGCTGCAC TATACCAGAC AATAATTGTC TGGCCTGTAC CGTCAGCGTC
          901 ATTGACGCTG CGCCCATAGT GCTTCCTGCT GCTCCCAAGA ACCCAAGGAA ACAAAGCTCC
          961 TATTTCCCAC TGCTCTTTTT TTTCTCTCTG CACCACTCTT CTCTTTTGCC CTTTGGTGGG
         1021 TGCCTACTCC TAATGGATTC AATTTTTTAC CTACCTCATA GTGATAATAA TCACTTCTCG
        1081 AACTGGTCCT TCAATATTCT CGTTCGATCA GGCTTCCTTG CAGGAT
        //
        Primer: EF1a-N_R (Pr0024)
        Region: EF-1a pro,cPPT_CTS,RRE
        Sequence file: RDB15443_A7Jve.seq check
        >D05018A3_A7Jv_2_BGH_rev2_A02_02_ABI24.ab1
            1 CCAGTTAGGA TCAGAGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA
           61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
          121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
          181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
          241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
          301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
          361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
          421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
          481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
          541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
          601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
          661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
          721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
          781 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG
          841 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA
          901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG
          961 GCGAGCAGCC ATGGAAAAGG ACGTCAGCTT TCCCCGACAC ACCACGGGAA TTGTCAGTGC
         1021 CCACAGCCGA GCCCCTGTCC AGCAGCGGGC AGCAGCGCGA TGAGGTTCGG TCGTGCATAG
         1081 GAGGGGGAAA GCGAAGGTCC GCAAGAGCCT GACAGTGTTG TATGCCTCCT AACTCAGTGA
        1141 G
        //
        Primer: BGH_rev2 (Pr0606)
        Region: bGHp(A),3'LTR,PRE
        Sequence file: RDB15443_A7Jvf.seq check
        >D05018A3_A7Jv_2_SV40pro_F_B02_05_ABI24.ab1
            1 ACCTTGTCCT GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
           61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
          121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
          181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
          241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
          301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
          361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
          421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
          481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
          541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
          601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
          661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
          721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
          781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
          841 TAATCATGGT CATAGCTGTT TCCTGGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
          901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
          961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCCAGCTGC
         1021 ATTAATGAAA TCGGGCCAAC GCGCGGGGAG AGCGGGTTTT GCGTATTGGG CGCTCTTCGG
         1081 CTTTCTCGCT CACTGAACTT CGCTTGCGCC TCGATTCGAT CCGGGCCTGG CGGACGAGCG
        1141 GGTAATTCCA GGCCTTCAAC T
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

        2023.11.23

        GNP_filter3_RDBDEP_html_231027.pl