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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-Myc-mG alpha i3

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i3, myc-tag.

Catalog number RDB15370
Resource name pCAG-Myc-mG alpha i3
Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i3, tagged with Myc at N-terminal, CAG promoter.
Comment The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnai3 cDNA
Depositor|Developer Nagata, Koh-ichi |
Other clones in our bank
External Database
mouse Gnai3
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15370 pCAG-Myc-mG alpha i3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15370_A7I5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V3
          Sequence file: RDB15370_A7I5a.seq check
          >D04868A3-1_A715_ pCAG-F_V3_F03_18_ABI24.ab1
              1 CCTTGGGGGC AGGCTGCTTC GGGGGGGGAG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT
             61 GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
            121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TTAATACGAC
            181 TCACTATAGG GAGACCCAAG CTAATCGATA TGGAGCAGAA GCTGATCTCC GAGGAGGACC
            241 TGATATCAAG CTTTGGATCC GGCTGCACGT TGAGCGCCGA GGACAAGGCG GCGGTGGAGC
            301 GGAGCAAGAT GATCGACCGC AACTTGCGGG AGGACGGGGA GAAAGCGGCC AAAGAAGTGA
            361 AGCTGCTGCT GCTCGGCGCT GGAGAATCTG GTAAAAGTAC CATCGTGAAA CAGATGAAAA
            421 TCATTCATGA GGACGGCTAT TCAGAGGACG AATGTAAACA GTATAAAGTA GTTGTCTACA
            481 GCAATACTAT TCAGTCCATC ATTGCAATCA TACGAGCCAT GGGACGGTTG AAGATTGATT
            541 TTGGGGAATC TGCCAGAGCA GATGATGCCC GACAGTTATT TGTTTTAGCT GGGAGTGCTG
            601 AAGAAGGAGT CATGACTTCA GAACTAGCAG GCGTGATTAA ACGTTTATGG CGAGATGGCG
            661 GGGTACAGGC ATGCTTTAGC AGGTCCAGGG AATATCAGCT CAATGATTCT GCTTCATACT
            721 ACCTAAATGA TTTGGATAGA ATATCCCAGA CCAACTACAT TCCAACTCAG CAAGATGTTC
            781 TTCGGACAAG AGTGAAGACT ACAGGCATTG TGGAGACCCA CTTCACCTTC AAGGAACTCT
            841 ACTTCAAAAT GTTTGATGTA GGTGGCCAAA GATCCGAACG AAAAAAAGTG GATTCACTGT
            901 TTTGAGGGAG TGACAGCAAT TATCTTTTGT GTGGCTCTCA GTGATTACGA CCTTGTTCTG
            961 GGCTGAAGGA TGAGGAAATG AAACCGAAAT GCATGAAAGC ATGAAATTGT TTGACAGCAT
           1021 TTGTAACAAC AAATGGTTTT ACAGACACTT CATCATCTCT TCTTTATTAG AAGACCTTTT
          1081 TGAGGAAAAA ATAGAGGAGT TCAATAACAA TCTGTTATCC AGGAAATACC CACTAAGGT
          //
          Primer: pCA-bgter
          Sequence file: RDB15370_A7I5b.seq check
          >D04868A3-1_A715_ pCA-bgter_C03_09_ABI24.ab1
              1 AGCTTAGAAA AGTCCAATAA TTTTTGGGCA GAGGGAAAAA GATCTCAGTG GTATTTGTGA
             61 GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG
            121 AATTCATACG ATTTAGGTGA CACTATAGAA TAGGGCCCTC TAGATGCATG CTCGAGGTTT
            181 AAACGCGGCC GCGGTACCGC TAGATCAATA AAGCCCACAT TCCTTTAAGT TGTTTTTAAT
            241 GATGACATCC GTAACAGCAT CAAAAACAAA CTGCACATTT TTGGTGTCTG TGGCACAGGT
            301 GAAGTGAGTG TAGACCTCCT TGGTATCTTT TCTTCGGTTC AGATCTTCAA ACTGGCACTG
            361 AATGTAAGCA GCTGCCTCTT CGTATGTATT GGAACCTGTG TATTCTGGAT AACAGATTGT
            421 TAATGGACTC CTCTTTATTT TTTCCTCAAA AAGGTCTTTC TTATTAAGAA AGAGAATGAT
            481 TGAAGTGTCT GTAAACCATT TGTTGTTACA AATGCTGTCA AACAATTTCA TGCTTTCATG
            541 CATTCGGTTC ATTTCCTCAT CCTCAGCCAG AACAAGGTCG TAATCACTGA GAGCCACACA
            601 AAAGATAATT GCTGTCACTC CCTCAAAACA GTGAATCCAC TTTTTTCGTT CGGATCTTTG
            661 GCCACCTACA TCAAACATTT TGAAGTAGAG TTCCTTGAAG GTGAAGTGGG TCTCCACAAT
            721 GCCTGTAGTC TTCACTCTTG TCCGAAGAAC ATCTTGCTGA GTTGGAATGT AGTTGGTCTG
            781 GGATATTCTA TCCAAATCAT TTAGGTAGTA TGAAGCAGAA TCATTGAGCT GATATTCCCT
            841 GGACCTGCTA AAGCATGCCT GTACCCCGCC ATCTCGCCAT AAACGTTTAA TCACGCCTGC
            901 TAGTTCTGAA GTCATGACTC CTTCTTCAGC ACTCCCAGCT AAAACAAATA AACTGTCGGG
            961 CATCATCTGC TCTGGCAGAT TCCCCAAAAT CAAATCTTTC AACCGTCCCA TGGCTCGTAT
           1021 GATTGCCAAT GATGGACTGA AATAGTATTG CTGGTAGACA ACTACTTTTA TACCTGTTTA
           1081 CAATTCGTCC TCTGAATAGG CCGGTCCCTC ATGATGATAT TCCATCTGAT TTCAGCGATT
          1141 GGGTAACCCT TTTTACCAGG ATTTCCTTCC CCAGACG
          //
          Primer: SV40pro_F
          Sequence file: RDB15370_A7I5c.seq check
          >D04868A3-1_A7I5_SV40pro_F_A02_02_ABI24.ab1
              1 GCTTGGGCCC GGGGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
            121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
            661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
            961 CGCCCAACCT GCCCATCACG AGATTTCGAT TTCCACCGCC GCCTTCTATG AAAGGTGGGC
           1021 TTCGGATCGT TTCCGGACGC GGCTGATGAT CTCAGCGCGG GATCTCATGC TGGAGGTCTC
          1081 GTCACCGAAC TTGGTATGCA GCTTATATGG TACAATAAAG CCGAT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl