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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hmKO-K/pRSETB

Plasmid clone of hmKO-K, faster-maturating version of Kusabira orange.

Catalog number RDB15314
Resource name hmKO-K/pRSETB
Alternative name hmKOkappa/pRSETB
Clone info. Plasmid clone of hmKO-K, faster-maturating version of Kusabira orange.
Vector backbone pRSET B (Plasmid)
Gene/insert name Fungia concinna Orange fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Fungia concinna Orange fluorescent protein

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            付加的提供条件:
            本件リソースの使用は学術機関での学術研究に限る。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15314 hmKO-K/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The hmKO-K/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15314).

            Reference section:

            Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15314_A7H9p1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp (Pr0261)
            Region: 6xHis,T7 tag,Xpress™ tag,insert 5'
            Sequence file: RDB15314_A7Htb.seq check
            >15314_15314_B4BqF_1_T7_20bp_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNTTT GTTNNNTTTA AGAAGGAGAN ATACATATGC GGGGTTCTCA
               61 TCATCATCAT CATCATGGTA TGGCTAGCAT GACTGGTGGA CAGCAAATGG GTCGGGATCT
              121 GTACGACGAT GACGATAAGG ATCCGACCAT GGTGAGCGTG ATCAAGCCCG AGATGAAGAT
              181 GAGGTACTAC ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATCGAGG GTGAGGGCAC
              241 AGGCAGACCT TACGAGGGAC ATCAGGAGAT GACACTGCGC GTCACAATGG CCGAGGGCGG
              301 GCCAATGCCT TTCGCCTTCG ACCTGGTGTC CCACGTGTTC TGTTACGGCC ACAGAGTTTT
              361 TACCAAGTAC CCAGAAGAGA TCCCAGACTA TTTCAAGCAG GCCTTTCCTG AGGGCCTGTC
              421 CTGGGAGAGG TCCCTGGAGT TCGAGGACGG CGGCTCCGCC TCCGTGAGCG CCCACATCAG
              481 CCTGAGGGGC AACACCTTCT ACCACAAGTC CAAGTTCACC GGCGTGAACT TCCCCGCCGA
              541 CGGCCCCATC ATGCAGAACC AGAGCGTGGA CTGGGAGCCC TCCACCGAGA AGANCACCGC
              601 CAGCGACNGC GTGCTGAAGG GCGACGTGAC CATGTACCTG AAGCTGGAGG GNGGCGGCAA
              661 CCACNAGTGN CAGTTCNAGA NCACCTACNN NNNCGNCNNN NNNNNCCTNN NNNNGNCCNG
            721 NNNNNNCNAC NTCNGNNNNN NNNGNNNNNN NNNN
            //
            Primer: f1ori_R3 (Pr0142)
            Region: T7 ter,insert 3'
            Sequence file: RDB15314_A7Htc.seq check
            >D04498J5_A7H9_1_f1ori_R3_H08_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNTN NNNNNNGNNN NNNNNNNNNN NNNCCTCTTC
               61 GCTATTACNN NCNGATCCGG ATATAGTTCC TCCTTTCAGC AAAAAACCCC TCAAGACCCG
              121 TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC
              181 TTCCTTTCGG GCTTTGTTAG CAGCCGGATC AAGCTTCGAA TTCTTAGGAG TGGGCCACGG
              241 CGTCCTCCAC CTGCTCGGTG ATGTTGCCCT CGGTCTTCCT CACCAGCCTG TGGCCGATGT
              301 AGTGGTCGCC GGGCATCTCC AGGATCTCCT TGGCGGCCTT GTAGGTGGTC TTGAACTGGC
              361 ACTTGTGGTT GCCGCCGCCC TCCAGCTTCA GGTACATGGT CACGTCGCCC TTCAGCACGC
              421 CGTCGCTGGC GGTGATCTTC TCGGTGGAGG GCTCCCAGTC CACGCTCTGG TTCTGCATGA
              481 TGGGGCCGTC GGCGGGGAAG TTCACGCCGG NNNNNNTGGA CTTGTGGTAG AAGGTGTTGC
              541 CCCTCAGGCT GATGTGGGCG CTCACGGAGG CGGAGCCGCC GTCCTCGAAC TCCAGGGACC
              601 TCTCCCAGGA CAGGCCCTCA GGAAAGGCCT GCTTGAAATA GTCTGGGATC TCTTCTGGGT
              661 ACTTGGTAAA AACTCTGTGG CCGTAACANA ACACGTGGGA CACCANNTCG AAGGNGAAAG
              721 GNATTGGCCC GCCCTCGGCC ATTGTGACGC GCAGTGTCAT CTCCTGATGT CCCTCGTAAG
              781 GTCTGCCTGT GCCCTCACCC TCGATTGTGA ACTCATGNCC ATTGACGGAN CCNTCCNTGT
              841 AGTANCTCAT CTTCATCTNN NNNCTTGATC ACGCTCACCA TGGGTCGGGA TCCTTATCGT
              901 CNTCNTCGTA CAGATNCNNN ANCCNTTTNC NGTCCACCAN NCNTGCTANC CNTANCATGN
              961 NNGNTNNNGA TGATGANNAN NCCCNNATNN GNNTATNNNC NNNNNNNNGN TNAANNNNNN
            1021 NNNTTTCTAN NGNNNAAANN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]

            2024.03.12

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