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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mAG-hGeminin (1/60) / pT2KXIGdeltain

hGem-based S/G2/M marker. for zebrafish cells to label nuclei with green fluorescent protein mAG.

Catalog number RDB15277
Resource name mAG-hGeminin (1/60) / pT2KXIGdeltain
Clone info. hGem-based S/G2/M marker. for zebrafish cells to label nuclei with green fluorescent protein mAG.
Vector backbone pT2KXIGdeltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name human Geminin cDNA Galaxeidae stony coral GFP cDNA
Reference of insert sequence AB505861 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
human Geminin Galaxeidae stony coral GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions:
          The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件:
          Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 提供条件は英文のまま記載してください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15277 mAG-hGeminin (1/60) / pT2KXIGdeltain DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The mAG-hGeminin (1/60) / pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15277).

          Reference section:

          Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Transposon and plasmid, Kawakami laboratory (English text)
          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15277_A8Hop1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-N_R2 (Pr0490)
          Region: insert 5', Tol2R
          Sequence file: RDB15277_A8Hoe.seq check
          >15277_15277_A8Ho_1_EF1a-N_R2_E11_14_ABI24.ab1
              1 TTTAACGGGC AAGATTGCAA GTTTGATTTG CATTGGAAAG GGTCGCTGGC TTTTGTGTTA
             61 CACGCCCCTT ATTTGTGCTT GATTAGATGA TCCCCCTGCT CGAGGGCCCA TCTGGCCTGT
            121 GTTTCAGACA CCAGGGAGTC TCTGCTCACG TTTCCTGCTA TTTGCAGCCT CTCTATCAAG
            181 ACTAATACAC CTCTTCCCGC ATCGGCTGCC TGTGAGAGGC TTTTCAGCAC TGCAGGATTG
            241 CTTTTCAGCC CCAAAAGAGC TAGGCTTGAC ACTAACAATT TTGAGAATCA GCTTCTACTG
            301 AAGTTAAATC TGAGGTTTTA CAACTTTGAG TAGCGTGTAC TGGCATTAGA TTGTCTGTCT
            361 TATAGTTTGA TAATTAAATA CAAACAGTTC TAAAGCAGGA TAAAACCTTG TATGCATTTC
            421 ATTTAATGTT TTTTGAGATT AAAAGCTTAA ACAAGAATCT CTAGTTTTCT TTCTTGCTTT
            481 TACTTTTACT TCCTTAATAC TCAAGTACAA TTTTAATGGA GTACTTTTTT ACTTTTACTC
            541 AAGTAAGATT CTAGCCAGAT ACTTTTACTT TTAATTGAGT AAAATTTTCC CTAAGTACTT
            601 GTACTTTCAC TTGAGTAAAA TTTTTGAGTA CTTTTTACAC CTCTGTCAAG AACTCCTGGA
            661 CAAACCTCTG ACCTGTGTGG AACAGAGTGG ATATGGGTGT CTGAACAGAT ATTCACGTCT
            721 TTTGCAGATC AGAGGGCATT TCTGGTGTTG TGCAATGACC TGGGTCCAAC AGGTAGGCGT
            781 ACTCGTATCC AAGAGCATTG TTGGACAGGA GGTAGTCTCC ATTCCTATAA AGAGGAAGGA
            841 ATGGCACCAT GTAGTAGCCG TCATTGTGGC CAATTGGTGC ATTGGTACGT GGGTAGATGG
            901 ACCGGGGAGG CTGATGAGTT CTTAGCCAGC GCTCAAAGAT GCTAGAAATG ATGTAAGAAA
            961 CAAATATGCA AGATGAAAGT TAAGATAAGA TAAAAAAAAT TTAAACAAAT AAATGCAATT
           1021 ATGTTGGTGT TTCCTATTTC CACCAATAAT TGCCTCACGC AGTAAAAGCC TAACAGAATA
          1081 TTTTTTTTTG ATTTCTTTTG CTTCTTGGCA AGGCCCAAAC CCCA
          //
          Primer: pAxCALNLF1 (Pr0166)
          Region: insert mid (mAG-hGeminin 1_60)
          Sequence file: RDB15277_A8Hof.seq check
          >15277_15277_A8Ho_1_pAxCALNL_F1_D11_11_ABI24.ab1
              1 AATAAAGATA TCTTATCATG TCTGGATCAT CATCGATTAA GATGTTAAGT GGTCATTCCG
             61 ATGTTTCCTT TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA
            121 TGCAGAAGGC TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC
            181 TTTGATTTCT TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA
            241 TGGATATCCC TTGGCCTGGC TGGGCAGCAT GGAGTACCTG GCCACGGCGT TCTCGTACAG
            301 CTTCACCTTG TTGTAGTCCT TGTCGTGCTT CAGGATCTCG ATCCTGTGGT CGATCTTGTG
            361 GGCGTCGGGC AGCCTCACCT CCTTCTTGGC CTTGTAGGTG GTCTTGAAGT CGCACCTGTA
            421 GTGGCCGCCG CCCTCCAGCA GCAGCCTCAT GTTCACGTCG CCCTTCAGCA CGCCGTCCTC
            481 CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCAGGGTC TTCTTCTGCA TCACGGGGCC
            541 GTTGGGGGGG AAGTTGGTGC CGTCGAACCT GATGTCGTAG AAGAAGCAGT CGCCCCTCAT
            601 GCTGATGTTG CTGGTGGCGG TGCAGATGCC CTGGTCCTCG TAGGTCATGC TCCTCTCCCA
            661 GTGGTAGCCC TCGGGGAAGG TCTGCTTGAA GTAGTCCTGG ATGTCGGCGG GGTACTTGGT
            721 GAAGGCCCTG TTGCCGTACT GGAACACGGT GGTCAGGATG TCGTAGGCGA AGGGCAGGGG
            781 GGCGCCCTCG GTCACGTTCA GGTCCAGGAT CTGGGTGCCC TCGTAGGGGT TGCCCTTGCC
            841 CTCGCCCTCG ATCACGAAGT TGTGGCCGTT CACGGTGCCC CTCATGCACA GCTTGATCTT
            901 CATCTCGGGC TTGATCACGC TCACCATGGT GGCGAAATTC CAGCACACTG GCGGCCGTTT
            961 ACTAGTGGAT CCGTCGAGGG AATTCTTTTG CCCAAAATGA TGAGACAGCA CAACAAACTC
           1021 AGCACGTTTG CCCAGGGAGC TGTAGGAAGA GAGACGGCAT GAACCATGTT AGCAGAGGGG
          1081 CCTGGATTGG AACTCAGAGT AACTTTATCG TCATCCTCAA ACAGGTG
          //
          Primer: pUC-CAPbs_up_F (Pr0536)
          Region: Tol2L, insert 3'
          Sequence file: RDB15277_A8Hog.seq check
          >15277_15277_A8Ho_1_pUC-CAP_bs_up_F_G11_20_ABI24.ab1
              1 GACGACAGTG GAGCGGGGCA GTGAGCGCAA CGCATTAATG TGAGTTAGCT CACTCATTAG
             61 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA
            121 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT
            181 CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCTCTAG AACTAGTGGA
            241 TCTGCTGGGC TTGCTGAAGG TAGGGGGTCA AGAACCAGAG GTGTAAAGTA CTTGAGTAAT
            301 TTTACTTGAT TACTGTACTT AAGTATTATT TTTGGGGATT TTTACTTTAC TTGAGTACAA
            361 TTAAAAATCA ATACTTTTAC TTTTACTTAA TTACATTTTT TTAGAAAAAA AAGTACTTTT
            421 TACTCCTTAC AATTTTATTT ACAGTCAAAA AGTACTTATT TTTTGGAGAT CACTTCATTC
            481 TATTTTCCCT TGCTATTACC AAACCAATTG AATTGCGCTG ATGCCCAGTT TAATTTAAAT
            541 GTTATTTATT CTGCCTATGA AAATCGTTTT CACATTATAT GAAATTGGTC AGACATGTTC
            601 ATTGGTCCTT TGGAAGTGAC GTCATGTCAC ATCTATTACC ACAATGCACA GCACCTTGAC
            661 CTGGAAATTA GGGAAATTAT AACAGTCAAT CAGTGGAAGA AAATGGAGGA AGTATGTGAT
            721 TCATCAGCAG CTGCGAGCAG CACAGTCCAA AATCAGCCAC AGGATCAAGA GCACCCGTGG
            781 CCGTATCTTC GCAGATCTGA TCTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT
            841 TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC
            901 AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCATAGACAT
            961 CACAAATTTC ACAATAAGCA TTTTTTTCAC TGCATTCTAG TTTGTGGTTT GTCCAAACTC
           1021 ATCATGTATC TTATCATGTC TGGATCATCA TCGATTAAGG AATGTAGTGG TCAATTTCCG
          1081 GAATGTTTCG TTTTTGGACC AGCTGGCCGG ACAAGCTCAA TTTCCTCCTT ACCAAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl