Please review the QC test results indicated by icon below as well as clone information before placing your order.

mKO2-zCdt1(1/190) / pT2KXIGdeltain

zCdt1-based G1 marker for zebrafish cells to label nuclei with orange fluorescent protein mKO2. A component of the zFucci.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]

提供案内 (日本国内) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15275_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: beta-gi_F () Sequence file: RDB15275_A7H9a.seq
>D004498I6_A7H9_1_beta-gi_F_H01_08_ABI08.ab1     1 CACAGGGGTC CAGGGCCTTC TTCTCTTTCC TACAGCTCCT GGGCAACGTG CTGGTTGTTG    61 TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGACGGATC CACTAGTAAC GGCCGCCAGT   121 GTGCTGGAAT TCGCCACCAT GGTGAGTGTG ATTAAACCAG AGATGAAGAT GAGGTACTAC   181 ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATTGAAG GTGAAGGCAC AGGCAGACCT   241 TACGAGGGAC ATCAAGAGAT GACACTACGC GTCACAATGG CCGAGGGCGG GCCAATGCCT   301 TTCGCGTTTG ACTTAGTGTC ACACGTGTTC TGTTACGGCC ACAGAGTATT TACTAAATAT   361 CCAGAAGAGA TACCAGACTA TTTCAAACAA GCATTTCCTG AAGGCCTGTC ATGGGAAAGG   421 TCGTTGGAGT TCGAAGATGG TGGGTCCGCT TCAGTCAGTG CGCATATAAG CCTTAGAGGA   481 AACACCTTCT ACCACAAATC CAAATTTACT GGGGTTAACT TTCCTGCCGA TGGTCCTATC   541 ATGCAAAACC AAAGTGTTGA TTGGGAGCCA TCAACCGAGA AAATTACTGC CAGCGACGGA   601 GTTCTGAAGG GTGATGTTAC GATGTACCTA AAACTTGAAG GAGGCGGCAA TCACAAATGC   661 CAAATGAAGA CTACTTACAA GGCGGCAAAA GAGATTCTTG AAATGCCAGG AGACCATTAC   721 ATCGGCCATC GCCTCGTCAG GAAAACCGAA GGCAACATTA CTGAGCAGGT AGAAGATGCA   781 GTAGCTCATT ACTCAATGTT GCCTTCCCAG GGCGGATATC CATCACACTG GCGGCCGCTC   841 GAGATGGCTC AAAGCTCGTG TTACTGATTA TTTTGCACAG AGTAAAAAAG CTGGTGTTTT   901 CTCGATCCTT TAACGGTCTA AGGGACAAAA GGTGTCCGGG GGATGTAGTA GAATCAGCCG   961 GTGATCAAAT AAACCCGAGG AGTTCGAGGC AGAGCGTCCC GGTTCCTCCA CGCAAAGCGT  1021 ACGCACAGTG AGATCACCAC AGCAGAAGCC GTCAGAAACA AACTCAGCTT GGAAGTTTTC  1081 TCCAAGTCAT CGAACGAGGG CTTTTATTCC ACCGGAAAAC CTGCAGAATA CCGCTCGGCG  1141 GGAATTCCGC GGTTGAAGTG AAAGATGGGA TGGACTTAAC GGGCTGAAAG TTCCCAGGAC 1201 ACCCCTCCTA AGCTCGGGAG AGTATTCTCT ACGCGGGATG ATGAT //
Primer: pAxCALNL_F1 () Sequence file: RDB15275_A7H9b.seq
>D04498I6_A7H9_1_pAxCALNL_F1_F05_17_ABI24.ab1     1 CTAAGGTATC TTATCATGTC TGGATCATCA TCGATTACTC TTTGCGGGCA GTTTGTTGAG    61 GTGCCTGAGG GCTGCTGCTG GCCAAGGGCT CAGTGGTCTT TACTTTGTCG TCGCTTGCCA   121 GTAGATAGAG TTTTTTCCTC GCAGTTTTCT GTCCAGCGCG CTCCTCCGGT GAGCTCCTGC   181 AGTTGTGGCC CGCGTTGAGT CTCTGCCGCT TCTTGGCGCT GCTATGCAGC TCGGCTGTGG   241 ACGGAAACAA CACCGAACAC ACGTCGAACT CCGGTGAACT CCGCTTAGGG GTCCTGGGAC   301 TCGCCGTTAA GCCCTCAATC TTCACATCAC GCGAATCCGC GACGGTATCT GCAGTCTCCG   361 TCGATAAAGC CTCGTCGATG ACTTTGAGAA ACTCCAGCTG AGTTTGTTTC TGCGGCTCTG   421 CTGTGGTGAT CTCACTGTGC GTCGCTTTGC GTGAAGAACC GGACGCTCTG CTCGAACTCC   481 TCGGTTTATT GATCACCGCT GATTCTACTA CATCCCCGGA CACCTTTTGT CCCTTAGACC   541 GTAAAGATCG AGAAACACCA GCTTTTTTAC TCTGTGCAAA ATAATCAGTA ACACGAGCTT   601 GAGCCATCTC GAGCGGCCGC CAGTGTGATG GATATCCGCC CTGGGAAGGC AACATTGAGT   661 AATGAGCTAC TGCATCTTCT ACCTGCTCAG TAATGTTGCC TTCGGTTTTC CTGACGAGGC   721 GATGGCCGAT GTAATGGTCT CCTGGCATTT CAAGAATCTC TTTTGCCGCC TTGTAAGTAG   781 TCTTCATTTG GCATTTGTGA TTGCCGCCTC CTTCAAGTTT TAGGTACATC GTAACATCAC   841 CCTTCAGAAC TCCGTCGCTG GCAGTAATTT TCTCGGTTGA TGGCTCCCAA TCAACACTTT   901 GGTTTTGCAT GATAGGACCA TCGGCAGGAA AGTTAACCCC CAGTAAATTT GGATTTGTGG   961 TAGAAGGTGT TTCCCTCTAA GCTTATATGC GCACTGACTG AAGCGGACCC ACCATCTTCG  1021 AACTCCACGA CCTTTCCCAT GACAGGCCTC AAGAAATGCC TGCTTGGAAT AGTCTGCAAT 1081 CTCTTCCTGC ATATTTTAGT AAATACCTCC CTG //
Primer: EF1a-N_R2 () Sequence file: RDB15275_A7H9c.seq
>D04498I6_A7H9_1_EF1a-N_R2_G05_20_ABI24.ab1     1 TTGCGGGCAA GATTGCAAGT TTGATTTGCA TTGGAAGGGT CGCTGGCTTT TGTGTTACAC    61 GCCCCTTATT TGTGCTTGAT TAGATGATCC CCCTGCTCGA GGGCCCATCT GGCCTGTGTT   121 TCAGACACCA GGGAGTCTCT GCTCACGTTT CCTGCTATTT GCAGCCTCTC TATCAAGACT   181 AATACACCTC TTCCCGCATC GGCTGCCTGT GAGAGGCTTT TCAGCACTGC AGGATTGCTT   241 TTCAGCCCCA AAAGAGCTAG GCTTGACACT AACAATTTTG AGAATCAGCT TCTACTGAAG   301 TTAAATCTGA GGTTTTACAA CTTTGAGTAG CGTGTACTGG CATTAGATTG TCTGTCTTAT   361 AGTTTGATAA TTAAATACAA ACAGTTCTAA AGCAGGATAA AACCTTGTAT GCATTTCATT   421 TAATGTTTTT TGAGATTAAA AGCTTAAACA AGAATCTCTA GTTTTCTTTC TTGCTTTTAC   481 TTTTACTTCC TTAATACTCA AGTACAATTT TAATGGAGTA CTTTTTTACT TTTACTCAAG   541 TAAGATTTCT AGCCCAGAAT AACTTTTTAC CTTTTTAAAA TTTGAGGGAT AAAAAATTTT   601 TTTCCCTCTA AGAAAAACTC TGTGTTGAAC ATCTCTCTCA CCTCTGTGGA GAAAAAAAAA   661 ATTTTTTGTG AGAGAAAACT CTTTTTTTTT ACCCCCCCCT GTCGGGAAAA AAAAAAATTC   721 CTCGTGGGAG AAAAAAAACC CCCCCGAAAC CCGGGGGGGG GGGGCCCCCA AGGGGGGGAA   781 AGGGGGGGGG GGGCGCCAAC AAAAAAATTT TACCCCCCCC CTTTTTTTTG GGAAAAAAAG   841 GGAAGGGGGC CCCTCTCGGG GGGGGGGGGG TGAGAAAAAA AAAAAACGCG GGGGGGCCAC   901 ACAAAGGGGG GGGGGGGGGG GACTCCCCTC TATCTTCAAA AAGATTTTTT TTTTTTTTTG   961 TGACAGAACA GAGAGAGAAC TCTCTCTTCT CTGTCATAAA AAAAGAAGAG AGGGGGGGGC  1021 GCCCCCCTCT GCTATATATA CCACCACTTT TTGGTGTGTC GCAGCACCAA TAGTGTGGGT  1081 CGTGCTTAGT CACTAGCCGG GCGCGTAAGA ATAGGATACG TCGACGAAGC CACAGAAGAT 1141 AATTATGATC CTC //
Primer: pAxEF_R2 () Sequence file: RDB15275_A7H9d.seq
>D004498I6_A7H9_1_pAxEF_R2_G01_07_ABI08.ab1     1 CCCCGGTAAA AATGCTTTAT TTGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA    61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG   121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT   181 ATGATCCTCT AGATCAGATC TGCGAAGATA CGGCCACGGG TGCTCTTGAT CCTGTGGCTG   241 ATTTTGGACT GTGCTGCTCG CAGCTGCTGA TGAATCACAT ACTTCCTCCA TTTTCTTCCA   301 CTGATTGACT GTTATAATTT CCCTAATTTC CAGGTCAAGG TGCTGTGCAT TGTGGTAATA   361 GATGTGACAT GACGTCACTT CCAAAGGACC AATGAACATG TCTGACCAAT TTCATATAAT   421 GTGAAAACGA TTTTCATAGG CAGAATAAAT AACATTTAAA TTAAACTGGG CATCAGCGCA   481 ATTCAATTGG TTTGGTAATA GCAAGGGAAA ATAGAATGAA GTGATCTCCA AAAAATAAGT   541 ACTTTTTGAC TGTAAATAAA ATTGTAAGGA GTAAAAAGTA CTTTTTTTTC TAAAAAAATG   601 TAATTAAGTA AAAGTAAAAG TATTGATTTT TAATTGTACT CAAGTAAAGT AAAAATCCCC   661 AAAAATAATA CTTAAGTACA GTAATCAAGT AAAATTACTC AAGTACTTTA CACCTCTGGT   721 TCTTGACCCC CTACCTTCAG CAAGCCCAGC AGATCCACTA GTTCTAGAGC GGCCGCCACC   781 GCGGTGGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC   841 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG   901 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT   961 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG  1021 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTTCTCGCT  1081 CACTGAACTC GCTGCGCTCG GTCGTTTCGG GCTGGCCGCA GCGTATCAGC TCACCTCAAG 1141 GGCGGTAATA CCGGGTTATC TCCACTCACA GGATATCTAG GGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.17

GNP_filter3_RDBDEP_html_230702.pl