Please review the QC test results indicated by icon below as well as clone information before placing your order.

mCherry-hCdt1(30/120)/ pCSII-EF

Lentivirus vector plasmid of Fucci2-G1 phase probe. Component of Fucci2 cell cycle probe.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【追加提供依頼書】受入れ確認書(書式J) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。提供条件の言語は英語のみ。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15273_A7H3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1alpha pro, insert5' Sequence file: RDB15273_A7H3a.seq
>D04498I4_2_A7H3_EF1a-C_F_G01_19_ABI24.ab1     1 TCATGGGATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT    61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTCGCC   121 ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC   181 AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG   241 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG   301 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG   361 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG   421 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG   481 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC   541 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC   601 GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC   661 GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC   721 GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC   781 GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GGGATATCCA   841 TCACACTGGC GGCCGCTCGA GCCCAGCCCC GCCAGGCCCG CACTCCGCGC CCCGGCCTCC   901 GCTACCAGTG GCAGCCGCAA GCGCGCCCGC CCGCCCGCCG CCCCCGGACG CGACCAGGCC   961 AGGCCACCGG GCCCGCAGAG ACTGCGGCTG TCGGTGGACG AGGTTTCCAG CCCCAGTACC  1021 CCCGGAGCCC CAGACATCCC AGCCCTGCCC TTCTCCGGGC CAGAAGATAA GAATCCACCC  1081 CCGGCAGCAG TCAGCCGCCC CCACTGACAT CCGCGCAGGA CAGACTCATC TATCTAGAGT 1141 TACATCGAAG GATCAAGGCT TTAATCGAAT AATTCAATGC //
Primer: PRE_rev (Pr0250) Region: PRE, insert3' Sequence file: RDB15273_A7H3b.seq
>D04498I4-2_A7H3_PRE_rev_G02_20_ABI24.ab1     1 ACGGCATTCA CATAGCGTAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA    61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT   121 AGATGGTGTC CTGGTCCTGC GCGGATGTCA GGTGGGGCGG CTGACCTGCT GCCGGGGTGG   181 ATTTCTTTAT CTTCTGGCCC GGAGAAGGGC AGGCTGGGAT GTCTGGGGCC TCGGGGGTAC   241 TGGGGCTGGA AACCTCGTCC ACCGACAGCC GCAGTCTCCT GCGGGCCGGT GGCCTGGCCT   301 GGTCGCGTCC GGGGGCGGCG GGCGGGCGGG CGCGCTTGCG GCTGCCACTG GTAGCGGAGG   361 CCGGGGCGCG GAGTGCGGGC CTGGCGGGGC TGGGCTCGAG CGGCCGCCAG TGTGATGGAT   421 ATCCCTTGTA CAGCTCGTCC ATGCCGCCGG TGGAGTGGCG GCCCTCGGCG CGTTCGTACT   481 GTTCCACGAT GGTGTAGTCC TCGTTGTGGG AGGTGATGTC CAACTTGATG TTGACGTTGT   541 AGGCGCCGGG CAGCTGCACG GGCTTCTTGG CCTTGTAGGT GGTCTTGACC TCAGCGTCGT   601 AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT GCTTGATCTC GCCCTTCAGG GCGCCGTCCT   661 CGGGGTACAT CCGCTCGGAG GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC   721 CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA   781 GGGAGGAGTC CTGGGTCACG GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC   841 ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA   901 CGTAGGCCTT GGAGCCGTAC ATGAACTGAG GGACAGGATG TCCCAGGCGA AAGGGCAGGG   961 GCCACCCTTG GTCACCTTCA GCTTGGCGGG TCTGGGTGCC CTCGTAGGGG CGGGCCCTCG  1021 CTCTCGCCCT CGATCTCGAA CTCGTGGCCG TTCACGGAGC CTCCATGTGC ACCTTTGAAG  1081 CGCATGGAAC TCCATGGATG ATGGGCCATG GTATCTTCCT CGCCCTTGCT CACCATGTGG 1141 CGATTCTCTC GGAACCGTAG CGATGTCACG GAACACATGC CTG //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5'LTR, HIV-1psi Sequence file: RDB15273_A7H3c.seq
>D04498I4-2_A7H3_CMV-Forward_G03_21_ABI24.ab1     1 TGGTCACCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT   121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA   181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA   241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA   301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG   361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT   421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG   481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG   541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA   601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT   661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT   721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG   781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT   841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG   901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGG AGCTTTGTTC CTTGGGTTCT TGGGAGCAGC   961 AGGAAGCACT ATGGGCGCAG CGTCAATGAC GCTGACGGTA CAGGCCAGAC ATTATTGTCT  1021 GGTATAGTGC AGCAGCAGAA CAATTTGCTG AGGGCTTATT GAGCGCAACA GCATCTGTTG  1081 CAACTCACAG TCTGGGGGCA ATCAAGCAGC TCAAGCCAAG AATCCTGGAC TGTGTGGAAA 1141 GGAAAATTAC CCTTAAAAAG //
Primer: BGH_rev2 (Pr0606) Region: bGH p(A), 3'LTR, PRE Sequence file: RDB15273_A7H3d.seq
>D04498I4-2_A7H3_BGH_rev2_G04_19_ABI24.ab1     1 CCTTTCAACG ATCAGGTAGC ACGGGGGAGG GGCAACAACA GATGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT   121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT   181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA   241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC   301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG   361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG   421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG   481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT   541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC   601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA   661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG   721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT   781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA   841 GGCGAAGACG CGGAAGAGGC CGCAGAGCCG GCAGCAGGCC GCGGGAAGGA AAGGTCCGCT   901 GGATTGAGGG CCGAAAGGGA CGTAGCAGAA GGACGTCCCG CGCAGAATCC AGGTGGCAAC   961 ACAGGCGAGC AGCCATGGGA AAGGGACGTC AGCTTCCCCG ACACACCACG GAATTGTCAG  1021 TGCCCAACAG CCGAGCCCCT GTCCAGCAGC GGGCAGCAGC GCGATGAGTC CGCCGTGTCA  1081 TAGGAGGGGG AAGCGAAGTC CGGATGGAGC TGACTGGTGG TGGCCATGCC CACCCAGCGT 1141 GGGGGGATTG GGCGTTCAGC //
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori, Zeo, SV40 p(A) Sequence file: RDB15273_A7H3e.seq
>D04498I4-2_A7H3_SV40pro_F_G05_20_ABI24.ab1     1 CCCGTCCCGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA    61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC   121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA   181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG   241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG   301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT   361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC   421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG   481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA   541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT   601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA   661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG   721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA   781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA   841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT   901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT   961 AATTTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT  1021 AAATGAATCG GCCCAACGCC GCGGGGGAGA GGCGGTTGCG TATTGGGGCG CTCTTCCGCT  1081 TCCTCGCCTC ACTGACTCGC TGCGCTCGGT TCGGTCCGGC CTGCCGGCGG AGCGGTAATC 1141 AGCCTCCACC TTCCAAAAGA GGCCCGGTTT AT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.04.04

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