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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Xpa

Plasmid clone of a photoswitchable variant of KikGR.

Catalog number RDB15264
Resource name Xpa
Clone info. Plasmid clone of a photoswitchable variant of Kikume Green-Red fluorescent protein (KikGR), h41pkve6 as Xpa (crystallizable and photo-activatable).
Vector backbone pcs4 (pCS2+ derivative) (Plasmid)
Selectable markers Amp^r
Gene/insert name Favia favus Kikume Green-Red fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Favia favus Kikume Green-Red fluorescent protein

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsutsui H, Jinno Y, Shoda K, Tomita A, Matsuda M, Yamashita E, Katayama H, Nakagawa A, Miyawaki A. (2015) A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsutsui H, Jinno Y, Shoda K, Tomita A, Matsuda M, Yamashita E, Katayama H, Nakagawa A, Miyawaki A. (2015) A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15264 Xpa DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The Xpa was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15264).

            Reference section:

            Tsutsui, H., Jinno, Y., Shoda, K., Tomita, A., Matsuda, M., Yamashita, E., Katayama, H., Nakagawa, A., Miyawaki, A., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15264_A7H9p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCALNLF1 ()
            Region: SV40 p(A),insert,SP6 pro.,CMVIE94 pro.
            Sequence file: RDB15264_A7H9a.seq check
            >D04498I4-2_A7H9_pAxCALNL_F1_D01_04_ABI08.ab1
                1 CAAGGGTATC CTTTATCATG TCTGGATCTA CGTAATACGA CTCACTATAG TTCTAGAGGC
               61 TCGAGAGGCC TTGAATTCTT ACTTGGCCAG CCTTGGCAGC CCGGAATGAG CTTTAGCATG
              121 CTCATACAGC TTAACCTTGT TGTAATCCTT GTCATGGCTT GTTATCTCCA TACTATGATC
              181 CACGAAGTGA TAGTCTGGCA ACTGGACAAC CTTCTTTGCT TTGTAAGTAG TTCTGAAGTC
              241 ACATCGGTAA TGGCCACCTC CTTGAAGCAA CAGAGCCATG TTTACATCAC CCTTCAGCAC
              301 TCCATCACGC ACATACATTT TCTCAGTGGA TGGCTCCCAT TTGACGGTCT TCCTCTGCAT
              361 AACTGGACCA TTGGCAGGAA AGTTCACACC ATCAAATCGA ATTTCATTGA CAAAACAGTT
              421 GCTGCCGTCT TTCTTCATCG TTATATTGTT TGTGGCGAGG CAAATTCCCC CGTCTTCGTA
              481 ACTCATGCTT CGTTCCCAAG AATAACCCTC AGGAAACGAC TGCTTGAAGT AGTCTACTAT
              541 TTCTTCTGGG TATTCGACAA ATACCCGGTT GCCGTAATGG AATGCTGTTG TCAGGATATC
              601 GAAAGCAAAA GGAAGAGGTC CGCCCTCTAT GACTGTCAGG TCCACATTCT GTATTCCCTC
              661 GAAAGGCTGG CCACTTCCTT TCCCTGTAAT CACGAACTTG TGCCCGTTTA CAGCGCCTTC
              721 CATACGCAGC TCGATCTTCA TTTCTGATGT AATCACACTC ACCATGGTCG GATCCTGCAA
              781 AAAGAACAAG TAGCTTGTAT TCTATAGTGT CACCTAAATC AAGCTTGCTC CGACGTCCCC
              841 AGGCAGAATG GCGGTTCCCT AAACGAGCAT TGCTTATATA GACCTCCCAT TAGGCACGCC
              901 TACCGCCCAT TTACGTCAAT GGGAACGCCC ATTTGCGTCA TTGCCCCTCC CCATTGACGT
              961 CAATGGGGGA TGTACTTGGC AGCCATCGCG GGCCCATTTA CCGGCCATTG AACGTCAATG
             1021 GGGAGTACTG CCCAATGGTA CCCTGGCCGT ACCTTTCCAA TAGTAAATGT AACTGCTAAG
             1081 CTACTAATTA GTAGGATATG ATGGTACTTG CGAGTTGGGG CCAATTTACC GATCCAATTG
            1141 A
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Tsutsui, H., A diffraction-quality protein crystal processed as an autophagic cargo. Mol. Cell 58 (1): 186-193 (2015). PMID 25773597. [link to RRC of NBRP]
            reference Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]

            2023.08.17

            GNP_filter3_RDBDEP_html_230702.pl