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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

KikGR/pRSETB

Photoswitchable green fluorescent protein, Kikume Green-Red (KikGR).

Catalog number RDB15259
Resource name KikGR/pRSETB
Clone info. Kikume Green-Red (KikGR), tetramer. It can be switched into red fluorescence upon illumination with UV or violet light.
Vector backbone pRSET B (Plasmid)
Gene/insert name Favia favus Kikume Green-Red (KikGR) fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank
External Database
Favia favus Kikume Green-Red (KikGR) fluorescent protein
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A. (2005) Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A. (2005) Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15259 KikGR/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The KikGR/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15259).

            Reference section:

            Tsutsui, H., Karasawa, S., Shimizu, H., Nukina, N., Miyawaki, A., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15259_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp (Pr0261)
            Region: RBS, 6XHis,T7 tag, Xprees tag, insert, MCS,T7 ter
            Sequence file: RDB15259_A7H9a.seq check
            >D04498G8-2_A7H9_T7_20bp_G12_21_ABI24.ab1
                1 GCGTAACCCG TCTAGGATAA TTTTGTTTAC TGTTAAGAAG GAGATATACA TATGCGGGGT
               61 TCTCATCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG
              121 GATCTGTACG ACGATGACGA TAAGGATCCG AGCGGCCGCA TGAGTGTGAT TACATCAGAA
              181 ATGAAGATCG AGCTGCGTAT GGAAGGCGCT GTAAACGGGC ACAAGTTCGT GATTACAGGG
              241 AAAGGAAGTG GCCAGCCTTT CGAGGGAATA CAGAATGTGG ACCTGACAGT CATAGAGGGC
              301 GGACCTCTTC CTTTTGCTTT CGATATCCTG ACAACAGCAT TCCATTACGG CAACCGGGTA
              361 TTTGTCGAAT ACCCAGAAGA AATAGTAGAC TACTTCAAGC AGTCGTTTCC TGAGGGTTAT
              421 TCTTGGGAAC GAAGCATGAG TTACGAAGAC GGGGGAATTT GCCTCGCCAC AAACAATATA
              481 ACGATGAAGA AAGACGGCAG CAACTGTTTT GTCAATGAAA TTCGATTTGA TGGTGTGAAC
              541 TTTCCTGCCA ATGGTCCAGT TATGCAGAGG AAGACCGTCA AATGGGAGCC ATCCACTGAG
              601 AAAATGTATG TGCGTGATGG AGTGCTGAAG GGTGATGTAA ACATGGCTCT GTTGCTTCAA
              661 GGAGGTGGCC ATTACCGATG TGACTTCAGA ACTACTTACA AAGCAAAGAA GGTTGTCCAG
              721 TTGCCAGACT ATCACTTCGT GGATCATCAA ATGGAGATAA CAAGCCATGA CAAGGATTAC
              781 AACAAGGTTA AGCTGTATGA GCATGCTAAA GCTCATTCCG GGCTGCCAAG GCTGGCCAAG
              841 TAACTCGAGA TCTGCAGCTG GTACCATGGA ATTCGAAGCT TGATCCGGCT GCTAACAAAG
              901 CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG
              961 GGGGCCTCTA ACGGGTCTTG AGGGGTTTTT TTTGCTGAAA GGAGACTATA TCCCGGATCT
             1021 GGGCGTAATA GCGTAGAGCC CGCAACGATC GGCCCTTCCA CCAGGTTGCC GCCAGCCTGA
            1081 AATGCGATGG ACGCGCTCTG TAGCGGCCGC AATTTAAGGC GCCG
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]

            2023.08.17

            GNP_filter3_RDBDEP_html_230702.pl