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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

cjBlue Y64L/pRSETB

Plasmid clone of yellow color protein, a variant of cjBlue of sea anemone Cnidopus japonicus.

Catalog number RDB15243
Resource name cjBlue Y64L/pRSETB
Clone info. Plasmid clone of yellow color protein, a variant of cjBlue. Bacterial expression.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Cnidopus japonicus blue color protein cDNA
Reference of insert sequence BD408956 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Cnidopus japonicus blue color protein

            Reference sequence
              
             
            Sequence (full) RDB15243zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15243 cjBlue Y64L/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The cjBlue Y64L/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15243).

            Reference section:

            Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Privé, G.G., Miyawaki, A., Ikura, M., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15243_A7H9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F (Pr0724)
            Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
            Sequence file: RDB15243_A7H9a.seq check
            >15243_B0L4F_1_lacIpro-B_F_C03_09_ABI24.ab1
                1 NNNNNNNNNN NNNNNCGCNN CGNGTCNGTG AGCGAGGAAG CGGAAGANCG CCCAATACGC
               61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
              121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
              181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
              241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA
              301 CCATGGCTTC CAAAATCAGC GACAATGTAC GTATCAAGTT ATATATGGAG GGCACAGTCA
              361 ACAATCATCA CTTCATGTGC GAAGCTGAAG GAGAGGGCAA GCCATACGAG GGAACTCAAA
              421 TGGAGAACAT AAAAGTCACC AAAGGAGGCC CTCTGCCGTT CTCTTTTGAT ATCTTGACGC
              481 CTAACTGCCA ACTCGGAAGC GTAGCCATAA CCAAGTATAC ATCAGGGATT CCAGACTACT
              541 TTAAGCAATC TTTTCCTGAA GGATTTACCT GGGAAAGAAC CACAATCTAC GAAGATGGGG
              601 CTTACCTTAC AACTCAACAA GAAACCAAAC TTGATGGAAA TTGCCTCGTC TACAATATTA
              661 AAATCCTTGG ATGTAATTTT CCCCCCAATG GTCCTGTGAT GCAGAAGAAA ACCCAAGGCT
              721 GGGAACCCTG TTGCGAGATG CGCTATACAC GTGATGGTGT GCTATGTGGC CAAACATTAA
              781 TGGCACTTAA ATGCGCCGAT GGGAACCACC TCACTTGCCA TCTGAGAACT ACTTACAGGT
              841 CCNAAAAGGC AGCAAAGGCG TTGCAGATGC CACCCTTCCN TTTTTCANAC CATCGNCCTG
              901 AAATAGTGAA NGNTTCANAN AACGGNNCAC TATTTGAACA GCANGAANGT TCAGNGGCCA
              961 GGTACTGNCN AACATGCCCA TCNAAACTNG GNCACNATTA AANGNANNAC ANNANNNNCN
             1021 TTNCNNNCNN NNNNNAACTT NNNTTNNNNN NNNNTTTTNN NNNNTTTNNC NNNNNNNNNN
            //
            Primer: cjB-M_F1 (Pr0958)
            Region: insert 3'
            Sequence file: RDB15243_A7H9b.seq check
            >15243_B0L4F_1_cjB-M_F1_B02_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNGNNNN NNNNNNNNCC CNCCAATGGT CCTGTGNTGC AGAAGAAAAC
               61 CCAAGGCTGG GAACCCTGTT GCGAGATGCG CTATACACGT GATGGTGTGC TATGTGGCCA
              121 AACATTAATG GCACTTAAAT GCGCCGATGG GAACCACCTC ACTTGCCATC TGAGAACTAC
              181 TTACAGGTCC AAAAAGGCAG CAAAGGCGTT GCAGATGCCA CCCTTCCATT TTTCAGACCA
              241 TCGTCCTGAA ATAGTGAAGG TTTCAGAGAA CGGCACACTA TTTGAACAGC ACGAAAGTTC
              301 AGTGGCCAGG TACTGTCAAA CATGCCCATC TAAACTTGGT CACAATTAAA GGACAACACT
              361 AGCTTCATTC CAACCAGGAC AAACTTAGTT TCATGGATAT TTTTAATAGT TTTTCCCTAT
              421 CAGTGTCCGA TTCCTAATTC CAATTCCTAA CTAGATATTT CATTTGAATT GTCATTGATT
              481 TATATGGCTG TAACATTGTG CATGCAACAA TTGTTTTCTG AGATTGATTG TGTCTTTGAA
              541 ATCAATAATA AACTCATACT TTTAAATTAA AAAAAAAAAA AAAAAAAAAA AAAAAATTCC
              601 TGCGGCCGCG AATTCNAAGC TTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGANTT
              661 GGCTGCTGCC ACCGCTGANC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT
              721 GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATCT GGCGTAATAG CNAANAGGCC
              781 CGCACCGATC GCCCTTCCCA ACAGTTGCGC ANCCTGANTG GNGAATGGGA CGCGCCCTGT
              841 ANCGNCNNAT TANGCGCGGC GGGNGNGGNG GNTACNCGCA GCGNNNNNGC TACACNNGNN
              901 AGCGCCCTAN CNCCNGCTCC TTTCNNTTNT TNCCTTCCTT TCNCGCNNCG TTCGCNGGNT
              961 TTNCCCGNNN AGNTCTAANT CNGGGNNNNC NTTTNNNNNN CNANTNNNNG NTTNNNNNNN
            1021 NCTCNNNCCN NNNNANNNGN NNNGNNGANG NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl