Please review the QC test results indicated by icon below as well as clone information before placing your order.

tdKeima/pRSETB

Plasmid clone of tdKeima.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15232_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15232_A7H9a.seq
>15232_B0L4F_2_lacIpro-B_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNCGNNN CGNNTCNGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC    61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT   121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC   181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA   241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA   301 CCATGGTGAG TGTGATCGCT AAACAAATGA CCTACAAGGT TTATATGTCA GGCACGGTCA   361 ATGGACACTA CTTTGAGGTC GAAGGCGATG GAAAAGGAAA GCCTTACGAG GGAGAGCAGA   421 CAGTAAAGCT CACTGTCACC AAGGGTGGAC CTCTGCCATT TGCTTGGGAT ATATTATCAC   481 CACTGTTTCA GTACGGAAGC ATACCATTCA CCAAGTACCC TGAAGACATC CCTGATTATG   541 TAAAGCAGTC ATTCCCTGAG GGATATACAT GGGAGAGGAC CATGAACTTT GAAGATGGTG   601 CAGTGTGTAC TGTCAGCAAT GATTCCAGCA TCCAAGGCAA CTGTTTCATC TACAATGTCA   661 AAATCTCTGG TACGAACTTT CCTCCCAATG GACCTGTTAT GCAGAAGAAG ACACAGGGCT   721 GGGAACCCAG CACTGAGCGT CTCTTTGCAC GAGATGGAAT GCTGATAGGA AACGATTATA   781 TGGCTCTGAA GTTGGAANGA GGTGGTCACT ATTTGTGTGA ATTTAAATCT ACTTACAAGG   841 CAAAGAANCC TGTGANGATG CCAGGGTATC ACTATATTGA CCGCAAACTG GATGTAACCA   901 GTCACAACAG GGNTTACACA TCTGTTGANC AGTGTGAAAT AGCNNTTGCA CGCCACTCTT   961 TGCTCGGNGG CGGCAGCGGC GGNGGNANCN GCNGNNNCGG CGGNANNNNN NCCGNCATNN 1021 NCNNNNCNAN NNNNNNNNN //
Primer: T7ter_R1 (Pr0798) Region: insert 3' Sequence file: RDB15232_A7H9b.seq
>15232_B0L4F_2_T7ter_R1_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN NTNANTGCTN NGCGGTGGCA GCAGCCAACT CAGCTTCCTT    61 TCGGGCTTTG TTAGCAGCCG GATCAAGCTT CGAATTCTTA GCCCAACAGG CTATGCCTTG   121 CAATGGCTAT TTCACACTGC TCAACAGATG TGTAATCCCT GTTGTGACTG GTTACATCCA   181 GTTTGCGGTC AATATAGTGA TACCCTGGCA TCCTCACAGG CTTCTTTGCC TTGTAAGTAG   241 ATTTAAATTC ACACAAATAG TGACCACCTC CTTCCAACTT CAGAGCCATA TAATCGTTTC   301 CTATCAGCAT TCCATCTCGT GCAAAGAGAC GCTCAGTGCT GGGTTCCCAG CCCTGTGTCT   361 TCTTCTGCAT AACAGGTCCA TTGGGAGGAA AGTTCGTACC AGAGATTTTG ACATTGTAGA   421 TGAAACAGTT GCCTTGGATG CTGGAATCAT TGCTGACAGT ACACACTGCA CCATCTTCAA   481 AGTTCATGGT CCTCTCCCAT GTATATCCCT CAGGGAATGA CTGCTTTACA TAATCAGGGA   541 TGTCTTCAGG GTACTTGGTG AATGGTATGC TTCCGTACTG AAACAGTGGT GATAATATAT   601 CCCAAGCAAA TGGCAGAGGT CCACCCTTGG TGACAGTGAG CTTTACTGTC TGCTCTCCCT   661 CGTAAGGCTT TCCTTTTCCA TCGCCTTCGA CCTCAAAGTA GTGTCCATTG ACCGTGCCTG   721 ACATATAAAC CTTGTAGGTC ATTTGCTTGG CAATGACGGA GANCATGCCG CCGCTGCCGC   781 CGGTACCGCC GCCGCTGCCG CCACCGAGCA AAGAGTGGCG TGCAATGGCT ATTTCACACT   841 GCTCAACAGA TGTGTAATCC CTGTTGTGAN TGGTTACATC CAGTTTGCGG TCAATATAGT   901 GATANCCTGG CATCCTCACA NGCTTCTTTG NCNTGNAAGT ANANTTAAAN TNACACAAAT   961 AGTGANNANC NCCTTCCNNC TTCNNANCCN TATANNCNNT TNNNATNNNC ATTNCATNNC 1021 NNGNNANNAN NNNNNCNNNN NNNGGNNNNC NNNNCNNNNN NNNNN //
Primer: dK-N_F1 (Pr0926) Region: insert mid Sequence file: RDB15232_A7H9c.seq
>15232_B0L4F_2_dK-N_F1_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNATGGNNNC TACTTTGAGG TCGAAGGCGA TGGAAAAGGA    61 AAGCCTTACG AGGGAGAGCA GACAGTAAAG CTCACTGTCA CCAAGGGTGG ACCTCTGCCA   121 TTTGCTTGGG ATATATTATC ACCACTGTTT CAGTACGGAA GCATACCATT CACCAAGTAC   181 CCTGAAGACA TCCCTGATTA TGTAAAGCAG TCATTCCCTG AGGGATATAC ATGGGAGAGG   241 ACCATGAACT TTGAAGATGG TGCAGTGTGT ACTGTCAGCA ATGATTCCAG CATCCAAGGC   301 AACTGTTTCA TCTACAATGT CAAAATCTCT GGTACGAACT TTCCTCCCAA TGGACCTGTT   361 ATGCAGAAGA AGACACAGGG CTGGGAACCC AGCACTGAGC GTCTCTTTGC ACGAGATGGA   421 ATGCTGATAG GAAACGATTA TATGGCTCTG AAGTTGGAAG GAGGTGGTCA CTATTTGTGT   481 GAATTTAAAT CTACTTACAA GGCAAAGAAG CCTGTGAGGA TGCCAGGGTA TCACTATATT   541 GACCGCAAAC TGGATGTAAC CAGTCACAAC AGGGATTACA CATCTGTTGA GCAGTGTGAA   601 ATAGCCATTG CACGCCACTC TTTGCTCGGT GGCGGCAGCG GCGGCGGTAC CGGCGGCAGC   661 GGCGGCATGG TCTCCGTCAT TGCNAAGCAA ATGACCTACA AGGTTTATAT GTCAGGCACG   721 GTCAATGGAC ACTACTTTGA GNTCGAAGGN GATGGAAAAG GAAAGCCTTA CGANGGAGAG   781 CAGACAGTAA AGCTCACTGT CACCNAGGNT GGANCTCTGC CATTTGCTTG GGATATATTA   841 TCACCACTGT TTCAGTACGG ANNCATANCA TTCACCANGT ACCCTGAANA NATCCNTGAT   901 TATGTAAAGC ANTCNTTCCC TGANGGATAT ACNTGGGAGA NGANCATGAA CTTTGAANAN   961 GGNGNAGTGT GTNNTGNCAG CNATGANNNC NGCATCCNNN NNNACTGTTT CNTCNNNNAN 1021 GNCNAAANTN NCNNGNNNNA NNNTTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl