Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFN18A-HP1a-Shadow-Halo

Expression vector of Drosophila Heterochromatin protein 1a (HP1a), Chromo Shadoe domain

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15212_A8Ksp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Halo-C_F Sequence file: RDB15212_A8Ksa.seq
>D04544B2_A8Ks_2_Halo-C_F_A06_03_ABI24.ab1     1 GGGCCGGACT TGCTGCAGAA GACACCCGGA CCTGATCGGC AGCGAGATCG CGCGCTGGCT    61 GTCGACGCTC GAGATTTCCG GCGAGCCAAC CACTGAGGAT CTGTACTTTC AGAGCGATAA   121 CGCGATCGCC CGCGGCCTGG AGGCCGAAAA GATCTTGGGT GCCTCCGACA ATAATGGCCG   181 CCTGACATTC CTCATTCAGT TCAAAGGCGT GGACCAAGCA GAAATGGTGC CCTCCTCAGT   241 GGCCAATGAA AAAATCCCAC GAATGGTAAT CCACTTCTAC GAAGAGCGCC TATCCGTTTA   301 AACGAATTCG GGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTG   361 ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT   421 AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG   481 GAACTATATC CGGTTCGCTT GCTGTCCATA AAACCGCCCA GTCTAGCTAT CGCCATGTAA   541 GCCCACTGCA AGCTACCTGC TTTCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC   601 AGTAGCTGAC ATTCATCCGG GGTCAGCACC GTTTCTGCGG ACTGGCTTTC TACGTAATGG   661 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT   721 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC   781 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT   841 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG   901 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA   961 AGATCCTTGA GAGTTTTCGC CCCGAAAGAA CGTTTTCCCA ATGATGAGCA CTTTCAAAGT  1021 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCCGG GCAAGAGCAA CTCGGGTCGG  1081 CGGCATACAC TATTCTCAGA ATGGACCTGT TGAGTACTTC ACCAGGTCAC AGAAAAAGGC 1141 ATCTTTAACC GGAAAT //
Primer: rrnBT1ter_F Sequence file: RDB15212_A8Ksb.seq
>D04544B2_A8Ks_2_rrnBT1ter_F_F01_06_ABI08.ab1     1 TGGGGGGGGG TTTTGAACGT TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC    61 GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC   121 GTTTCTACAA ACTCTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGG   181 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGTA TTTTAACTTT ACTAAGGAGA   241 ATTCATCATG GCAGAAATCG GTACTGGCTT TCCATTCGAC CCCCATTATG TGGAAGTCCT   301 GGGCGAGCGC ATGCACTACG TCGATGTTGG TCCGCGCGAT GGCACCCCTG TGCTGTTCCT   361 GCACGGTAAC CCGACCTCCT CCTACGTGTG GCGCAACATC ATCCCGCATG TTGCACCGAC   421 CCATCGCTGC ATTGCTCCAG ACCTGATCGG TATGGGCAAA TCCGACAAAC CAGACCTGGG   481 TTATTTCTTC GACGACCACG TCCGCTTCAT GGATGCCTTC ATCGAAGCCC TGGGTCTGGA   541 AGAGGTCGTC CTGGTCATTC ACGACTGGGG CTCCGCTCTG GGTTTCCACT GGGCCAAGCG   601 CAATCCAGAG CGCGTCAAAG GTATTGCATT TATGGAGTTC ATCCGCCCTA TCCCGACCTG   661 GGACGAATGG CCAGAATTTG CCCGCGAGAC CTTCCAGGCC TTCCGCACCA CCGACGTCGG   721 CCGCAAGCTG ATCATCGATC AGAACGTTTT TATCGAGGGT ACGCTGCCGA TGGGTGTCGT   781 CCGCCCGCTG ACTGAAGTCG AGATGGACCA TTACCGCGAG CCGTTCCTGA ATCCTGTTGA   841 CCGCGAGCCA CTGTGGCGCT TCCCAAACGA GCTGCCAATC GCCGGTGAGC CAGCGAACAT   901 CGTCGCGCTG GTCGAAGAAT ACATGGGACT GGCTGCACCA GTCCCCTGTC CCGAAGCTGC   961 TGTTCTGGGG GCACCCCAGG CGTTCTGATC CCACCGGGCC GAAGCCCGCT CGGCTTGACA  1021 AAAGCCTGCC CTAACTGCAA GCTGTGGACA TCGGCCGGTC TGAATCTGCT GCAAGGAAGA 1081 ACCCCTGACC TTGATTCGGC AGCCGAAGAA TCGCGCGCTT GGCTTGTTCT CGTCG //
Primer: pTrcHis-R Sequence file: RDB15212_A8Ksc.seq
>D04544B2_A8Ks_2_pTrcHiS-R_B06_06_ABI24.ab1     1 CGGGCCGTAA GAACTCGATC CTCTACGCCG GACGCATCGT GGCCGGAGTA CTGCGGTTTA    61 TCTCGATGGC TACGAGGGCA GACAGTAAGT GGATTTACCA TAATCCCTTA ATTGTACGCA   121 CCGCTAAAAC GCGTTCAGCG CGATCACGGC AGCAGACAGG TAAAAATGGC AACAAACCAC   181 CCGAAAAACT GCCGCAATCG CGCCTGATAA ATTTTAACCG TATGAATACC TATGCAACCA   241 GAGGGTACAG GCCACATTAC CCCCACTTAA TCCACTGAAG CTGCCATTTT TCATGGTTTC   301 ACCATCCCAG CGAAGGGCCA TCCAGCGTGC GTTCCTGTAT TTCCGGACTA TAAAAGGATC   361 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC   421 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAAATCC TTTTTTTCTG   481 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG   541 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA   601 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG   661 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG   721 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA   781 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC   841 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT   901 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGGAAACGC   961 CTGGTATCTT TATAGTCCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT  1021 GATGCTCGTC AGGGGGGCGG AGCCCTATGG AAAAACGCCA GCACGCGGGC CTTTTTTAAC  1081 GGATCTGGCC TTTTGCTGGG CCGTATGGCT TCACATGGAT CGTTCCTGCG TATCCCCTGG 1141 AATCTGTGAT ACGCTATTAC GGCCTTTTTT TGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl