Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFN18A-MCM10-ID-term-Halo

Expression vector of Drosophila Minichromosome maintenance protein 10 (MCM10), Internal Domain

Catalog number RDB15207
Resource name pFN18A-MCM10-ID-term-Halo
Clone info. Expression vector of Drosophila truncated form of Minichromosome maintenance protein 10 (MCM10) [Internal Domain, 241 to 430 a.a], tagged with Halo, see Fig. 4A of Vo, N. et al. Nucleic Acids Res., 45 (3): 1233-1254, 2017.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pFN18A Flexi (plasmid)
Selectable markers Amp^r
Gene/insert name Drosophila Mcm10 cDNA
Depositor|Developer Yamaguchi, Masamitsu |
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External Database
Drosophila Mcm10

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017)。
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          RDB15207 pFN18A-MCM10-ID-term-Halo DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15207_A8Ksp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Halo-C_F
          Sequence file: RDB15207_A8Ksa.seq check
          1
          Primer: T7 terminator
          Sequence file: RDB15207_A8Ksb.seq check
          1
          Primer: T7_-20bp
          Sequence file: RDB15207_A8Ksc.seq check
          1
          Primer: pTrcHiS-R
          Sequence file: RDB15207_A8Ksd.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

          2022.05.17

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