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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse VAMP-2

Expression vector of mouse vesicle-associated membrane protein 2 (VAMP-2).

Catalog number RDB15171
Resource name pEF-FLAG-mouse VAMP-2
Clone info. Expression vector of mouse vesicle-associated membrane protein 2 (VAMP-2), tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Vamp2 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Vamp2

          Reference sequence
            
           
          Sequence (partial) RDB15171pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 277 (33), 30351-30358, 2002)
          Additional terms and conditions:
          Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 277 (33), 30351-30358, 2002)。
          付加的提供条件:
          pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15171 pEF-FLAG-mouse VAMP-2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15171_A7Fmp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: EF1a pro, FLAG, insert, hGCSF+p(A)
          Sequence file: RDB15171_A7Fma.seq check
          >D04709A6_2_A7Fm_EF1a-C_F_C02_08_ABI24.ab1
              1 CCTGGGATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
             61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
            121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCATGTCGG CTACCGCTGC
            181 CACCGTCCCG CCTGCCGCCC CGGCCGGCGA GGGTGGCCCC CCTGCACCTC CTCCAAACCT
            241 TACTAGTAAC AGGAGACTGC AGCAGACCCA GGCCCAGGTG GATGAGGTGG TGGACATCAT
            301 GAGGGTGAAT GTGGACAAGG TCCTGGAGCG GGACCAGAAG TTGTCGGAGC TGGATGACCG
            361 TGCAGATGCC CTCCAGGCAG GGGCCTCCCA GTTTGAAACA AGTGCAGCCA AGCTCAAGCG
            421 CAAATACTGG TGGAAAAACC TCAAGATGAT GATCATCTTG GGAGTGATCT GCGCCATCAT
            481 CCTCATCATC ATCATCGTTT ACTTCAGCAC TTAAAATCAC TAGTGAATTC GCGGCCGCCT
            541 GCAGGTCGAC CATAGCGGCC GCTCTAGAGT GAGGGTCCCC ACCTGGGACC CTTGAGAGTA
            601 TCAGGTCTCC CACGTGGGAG ACAAGAAATC CCTGTTTAAT ATTTAAACAG CAGTGTTCCC
            661 CATCTGGGTC CTTGCACCCC TCACTCTGGC CTCAGCCGAC TGCACAGCGG CCCCTGCATC
            721 CCCTTGGCTG TGAGGCCCCT GGACAAGCAG AGGTGGCCAG AGCTGGGAGG CATGGCCCTG
            781 GGGTCCCACG AATTTGCTGG GGAATCTCGT TTTTCTTCTT AAGACTTTTG GGACATGGTT
            841 TGACTCCCGA ACATCACCGA CGTGTCTCCT GTTTTTCTGG GTGGCCTCGG GACACCTGCC
            901 CTGCCCCCAC GAGGGTCAGG ACTGTGACTC TTTTTAGGGC CAGGCAGGTG CCTGGACATT
            961 TGCCTTGCTG GACGGGGACT GGGGATGTGG GAGGGAGCAG ACAGGAGGAA TCATGTCAGG
           1021 CCTGTGTGTG AAAGGAAGCT CCACTGTCAC CCTCACTCTT CACCCCCCCA CCTCACCAGT
           1081 GTCCCCTCAC TGTCACATTG TAACTGAACG TCAGATTATT AAAGTGTTTG CCTCCGCAAA
          1141 AGAAAAAAAC AGAACAGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209.

          2022.10.11

          GNP_filter3_RDBDEP_html_221010.pl