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mCherry-EF1A1

Expression vector of rat Elongation factor 1-alpha 1 (EF1A1), mCherry tag.

Catalog number RDB15157
Resource name mCherry-EF1A1
Clone info. Expression vector of rat Elongation factor 1-alpha 1 (EF1A1) with N-terminally tagged mCherry.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Eef1a1 cDNA
Depositor|Developer Hamachi, Itaru |
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External Database
rat Eef1a1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Methods 13 (11): 931-937, 2016)。
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          RDB15157 mCherry-EF1A1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The mCherry-EF1A1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15157).

          Reference section:

          Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15157_A7Flp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: mCherry-C_F
          Sequence file: RDB15157_A7Fla.seq check
          >D04425A6_A7Fl_2_mCherry-C_F_D09_12_ABI24.ab1
              1 TGACAGGTAG AGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT
             61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
            121 CCGGCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TCGAGCTATG GGAAAGGAAA
            181 AGACTCACAT CAACATCGTC GTAATTGGAC ACGTAGATTC CGGCAAGTCC ACCACAACCG
            241 GCCACCTGAT CTACAAATGT GGTGGAATCG ACAAAAGAAC CATTGAAAAG TTTGAGAAGG
            301 AGGCTGCGGA GATGGGAAAA GGCTCCTTCA AGTATGCCTG GGTCCTGGAC AAACTGAAAG
            361 CTGAGCGTGA GCGTGGTATC ACTATTGACA TCTCCCTGTG GAAGTTTGAG ACCAGCAAGT
            421 ACTATGTGAC TATCATTGAT GCCCCAGGAC ACAGAGACTT CATCAAGAAC ATGATTACGG
            481 GCACATCTCA GGCTGACTGC GCTGTCCTGA TTGTTGCTGC TGGTGTTGGG GAATTTGAAG
            541 CTGGTATCTC CAAGAACGGG CAGACCCGTG AGCATGCTCT TCTGGCTTAC ACTTTGGGTG
            601 TGAAACAGCT AATTGTTGGT GTCAACAAAA TGGATTCCAC CGAGCCACCA TACAGTCAGA
            661 AGAGATACGA GGAAATCGTT AAGGAAGTCA GCACCTACAT TAAGAAAATT GGCTACAACC
            721 CTGACACAGT AGCATTTGTG CCAATTTCTG GTTGGAATGG TGACAACATG CTGGAGCCAA
            781 GTGCTAATAT GCCGTGGTTC AAGGGATGGA AAGTCACCCG CAAAGATGGC AGTGCCAGTG
            841 GCACCACGCT GCTGGAAGCT TTGGACTGCA TTCTGCCGCC AACTCGTCCA ACTGACAAGC
            901 CTCTGCGACT GCCCCTCCAG GATGTCTATA AAATTGGCGG CATTGGCACT GTCCCTGTGG
            961 GCCGAGTGGA AACTGGTGTT TCTCAAACCT GGTATGGGTG GTTACCCTTT GCTCCAGTCA
           1021 ATGTTAACAC TGAAGTCAAG TCTGTGGAAA TTGCACATGC AGGCTTTGAG TGAAGCTCTG
          1081 CCTGGGCACG ATTGTAAGCC TTCCAACGGT AAAAGAAACC GGGTGGT
          //
          Primer: SV40polyA-R
          Sequence file: RDB15157_A7Flb.seq check
          >D04425A6_A7Fl_2_SV40polyA-R_E09_15_ABI24.ab1
              1 AAAAGTTGGG AAGTTTTTTA AGCAAGTAAA CCTCTACAAA TGTGGTATGG CTGATTATGA
             61 TCAGTTATCT AGATCCGGTG GATCCTCATT TAGCCTTCTG AGCTTTCTGG GCAGACTTGG
            121 TGACTTTGCC AGCTCCTGCA GCCTTCTTGT CCACGGCTTT GATGACACCC ACAGCAACTG
            181 TCTGCCTCAT GTCACGAACA GCAAAACGAC CAAGTGGAGG GTAGTCAGAG AAGCTTTCAA
            241 CACACATGGG CTTGCCAGGG ACCATGTCAA CAATGGCAGC ATCACCAGAC TTCAAGAATT
            301 TGGGGCCATC TTCCAGCTTC TTACCAGAAC GACGATCGAT CTTCTCTTTA AGCTCGGCAA
            361 ACTTGCATGC TATGTGGGCC GTGTGGCAGT CCAGAACAGG GGCATAGCCA GCACTGATCT
            421 GGCCTGGATG GTTCAGGATA ATCACCTGAG CAGTGAAGCC AGCTGCTTCC ATTGGTGGGT
            481 CATTTTTGCT GTCCCCAGCA ACATTGCCAC GTCTAACGTC TTTGACAGAC ACGTTCTTTA
            541 CGTTGAAGCC TACATTGTCC CCAGGCAGAG CTTCACTCAA AGCTTCATGG TGCATTTCCA
            601 CAGACTTGAC TTCAGTTGTT ACATTGACTG GAGCAAAGGT AACCACCATA CCAGGTTTGA
            661 GAACACCAGT TTCCACTCGG CCCACAGGGA CAGTGCCAAT GCCGCCAATT TTATAGACAT
            721 CCTGGAGGGG CAGTCGCAGA GGCTTGTCAG TTGGACGAGT TGGCGGCAGA ATGCAGTCCA
            781 AAGCTTCCAG CAGCGTGGTG CCACTGGCAC TGCCATCTTT GCGGGTGACT TTCCATCCCT
            841 TGAACCACGG CATATTAGCA CTTGGCTCCA GCATGTTGTC ACCATTCCAA CCAGAAAATT
            901 GGCACAAATG CTACTGTGTC AAGGGTTGTA GCCAATTTTC TTAATGTAGG TGCTGACTTC
            961 CTTAACGAAT TTTCCCTCGT AATCTCTTCT GACTGTATGG GTGGGCTCGG TGGAAATCCA
           1021 ATTTTTTGGT TGACAACCAA CAAATTTAAG CTTGATTCAC AACCCAAAAG TGTAAGTCAG
          1081 AGAGCCATGC TTCAACGGTC TGGCCCCGGA TTTCCTTTGG GAG
          //
          Primer: CMV-Forward
          Sequence file: RDB15157_A7Flc.seq check
          >D04425A6_A7Fl_2_CMV-Forward_C09_09_ABI24.ab1
              1 CTGATCCCTT CCGCCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
             61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG
            121 GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG
            181 CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC
            241 CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC
            301 GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC
            361 GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG
            421 ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC
            481 GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG
            541 CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC
            601 CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG
            661 GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC
            721 ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC
            781 GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT
            841 CGAGCTATGG GAAAGGAAAA GACTCACATC AACATCGTCG TAATTGGACA CGTAGATTCC
            901 GGCAAGTCCA CCACAACCGG CCACCTGATC TACAAATGTG GTGGAATCGA CAAAAGAACC
            961 ATTGAAAAGT TTGAGAAAGG AGGCTGCGGA GATGGGAAAA GGCTCCTTCA AGTATGCCTG
           1021 GTCCTGCACA ACTGAAAGCT GAACGTGAGC GTGGTATCAC TATTGACATC TCCCTGTGGA
          1081 GTTTGAGACC AGCCAAGGCT ACCTAAG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15157_A7Fld.seq check
          >D04425A6_A7Fl_2_SV40pro_F_V2_F09_18_ABI24.ab1
              1 TCCCAACTTT TTTTGGGAGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
             61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
            601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
            901 CGCCCCAACC TGCCATCACG AGATTTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
            961 GCTTCGGAAT CGTTTTCCGG GGACGCCGGC TGGATGATCC TTCCAGCGCG GGGAATCTCA
           1021 TGCTGAGGTT CTTTCGCCCC ACCCTAAGGG GAGGCTAACT GAAACAACGG AAGGAGACCA
          1081 ATACCGGAAG GATCCCGGCC GCTTATTGAA CC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

          2023.12.13

          GNP_filter3_RDBDEP_html_231213.pl