Calcium ion sensor clone Ratiometric pericam having a circularly permuted YFP and a CaM.
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Other clones in our bank
External Database X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
提供条件 | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word] |
備考 | 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15143 | Ratiometric pericam/pcDNA3 | DNA solution |
Materials & Methods section:
The Ratiometric pericam/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15143). |
Reference section:
Nagai, T., Sawano, A., Park, E.S., Miyawaki, A., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Calcium Ion Sensor Clones (English text) |
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15143_A7H9p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward Sequence file: RDB15143_A7H9a.seq |
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>D04498D1_A7H9_1-CMV-Forward_G07_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNNGGCGGT AGGCNNTGTA NGGTGNNNNG 61 TCTATATAAG CAGAGCTCTC TGGCTAACTA NANANCCCAC TGCTTACTGG CTTATCGAAA 121 TTAATACGAC TCACTATAGG GAGACCCAAG CTTGGTACCG AGCTCGGATC CACCATGAAG 181 AGGCGCTGGA AGAAAAACTT CATTGCCGTC AGCGCTGCCA ACCGGTTCAA GAAGATCTCC 241 AGCTCCGGGG CACTGGGGTC TGCAGGCTAC AACAGCGACA ACGTCTATAT CATGGCCGAC 301 AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC 361 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG 421 CCCGACAACC ACTACCTGAG CTTCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC 481 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG 541 CTGTACAAGG TCGACGGTGG CAGCGGTGGC ACCGGTGTGA GCAAGGGCGA GGAGCTGTTC 601 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC 661 GTGTCCGGCG AGGGCGNNGG CGATGCCACC TACGGCAAGC TGACCCTGAA NNNCNNNNGC 721 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCNNGA CCACCTTCGG CTACGGCCTG 781 AANTGCTTCN CCCGCTACCC CGACCACATG AAGCANCANN ACTTCTTCAA NNTCCGCCAT 841 GCNNNNNGGC TACNTCCANG NANCGCACCA TCTTCTNNAA GGACNANGGC NANNNNCNAG 901 ACCCGCGCCN ANNNNAAGTT CNAGGGCGAN ANCCTGGTGA ANNNCNTCNN NCTNANNGGC 961 ATCGNNNTNN ANGNNNNANN GGCNACNNNC NGGGGCNNAA NNCTNGNNNN TANNANNGGT 1021 ACCNNANCNN NCNNNNNNNA NNANNNNANN NNNNNNNN // |
Primer: BGH_rev2 Sequence file: RDB15143_A7H9b.seq |
>D04498D1_A7H9_1-BGH_rev2_H07_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GGNNGNNGGG NAAACNACAG ATGGCTGGCA ACTAGAAGGC 61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT 121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATATCTG CAGAATTCTT ACTTTGCTGT 181 CATCATTTGT ACAAACTCTT CATAGTTTAC TTGGCCATCA CCATCGATAT CTGCTTCCCT 241 TATCATTTCA TCAACTTCTT CATCTGTTAA CTTCTCCCCG AGGTTTGTCA TGACGTGACG 301 TAACTGAGCA GCGCTGATGT AGCCGTTCCC ATCCTTGTCA AAAACACGGA ATGCTTCTCG 361 GATTTCCTCT TCGCTGTCTG TGTCCTTCAT TTTTCTAGCC ATCATAGTAA GAAATTCAGG 421 AAAGTAAATC GTTCCATTGC CATCAGCATC GACTTCATTG ATCATATCCT GCAATTCTGC 481 TTCCGTTGGG TTTTGTCCAA GCGACCTCAT AACGGTGCCA AGTTCCTTTG TGGTGATGGT 541 GCCGTCCCCA TCCTTGTCGA ATAATGAGAA GGCTTCTTTG AACTCTGCAA TCTGCTCTTC 601 TGTCAGTTGG TCGGTACCGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT 661 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC 721 GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAANATG GTGCGCTCCT GGACGTAGCC 781 TTCGGGCATG GCGGANTTGA ANAAGTCNTG CTGCTTCATG TGGTCNGGGT ANCGGGCGAA 841 GCACTTCANN CCGTAGCCGA ANGTGGTCNN GANGGTGGGC CANGGCACGG GCAGCTNGNN 901 NGTGGTGCAG ANGANCTTCN NGGTCAGCTT GCCGTANGTG GCATCGCCNN CGCCNNNNNN 961 NGANNNGCTG AACTTGNGNN NNTTNANNNC NNCNNNCCAN CTNNNNCANG ANNGGNCNNN 1021 NNNNNNN // |
Primer: pricam-M_R Sequence file: RDB15143_A7H9c.seq |
>15143_15143_B1IlF_pericam-M_F_E02_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN GNNGCCCTTC NGCTCGATGC GGTTCNCCAG GGTGTCGCCC 61 TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC 121 TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG 181 GGGTAGCGGG CGAAGCACTT CAGGCCGTAG CCGAAGGTGG TCACGAGGGT GGGCCAGGGC 241 ACGGGCAGCT TGCCGGTGGT GCAGATGAGC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG 301 CCCTCGCCCT CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC 361 AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACACCGGT GCCACCGCTG 421 CCACCGTCGA CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC 481 TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGAAG 541 CTCANGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG 601 TANTGGTCGG CGANCTGCAC GCCGCCGTCC TCGATGTTGT GNNGGANCTT GAAGTTGGNC 661 TTGATGCCGT TCNTCTGCNN GNCNGNCNNN GNNATANACN TTGNCNNTNN NNNANNNNNN 721 NANCCNNNNN NCNNNNNNNN NN // |
Primer: SV40pro_F Sequence file: RDB15143_A7H9d.seq |
>D04498D1_A7H9_1-SV40pro_F_A08_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNANN NGTGANGNNG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT 121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC 661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 721 AAATGGCCGC TTTTCTGGAN TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 781 NGACATAGCG TTGGCTACNC GTGATATTGC TGAAGANCTT GGCGGCGAAT GGGCTGANCG 841 CTTNCTCGNG CTTTACNGNA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT 901 TNNNNNCGAN TTCNTCNGAN CGGNNNNNNG GGNNTCNAAN TNANNNANNN AGCNNNNCCN 961 NNNCNNNNCN NNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. |
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reference | Fukano, T., Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters. Biochem. Biophys. Res. Commun. 3340 (1): 250-255 (2006). PMID 16360639. |
reference | Fukano, T., Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights. Biochem. Biophys. Res. Commun. 317 (1): 77-83 (2004). PMID 15047150. |
2023.05.01
GNP_filter3_RDBDEP_html_230501.pl