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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS coupler 5

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Catalog number RDB15130
Resource name pBS coupler 5
Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
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            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15130 pBS coupler 5 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pBS coupler 5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15130).

            Reference section:

            Shimozono, S., Miyawaki, A., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15130_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13R (Pr0160)
            Region: insert, f1 ori
            Sequence file: RDB15130_A7H9a.seq check
            >D04498B6_A7H9_1_M13R_D03_12_ABI24.ab1
                1 CTGGCGGCCA TTTAACCCCT CACTAAAGGG AACAAAAGCT GGGTACCGGG CCCCCCCTCG
               61 AGGTCGACGG TATCGATAAG CTTGATGCGA TCCGCCACCG CCAGAGCCAC CTCCGCCTGA
              121 ACCGCCTCCA CCATCGAATT CCTGCAGCCC GGGGGATCCA CTAGTTCTAG AGCGGCCGCC
              181 ACCGCGGTGG AGCTCCAATT CGCCCTATAG TGAGTCGTAT TACGCGCGCT CACTGGCCGT
              241 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC
              301 ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA
              361 ACAGTTGCGC AGCCTGAATG GCGAATGGAA ATTGTAAGCG TTAATATTTT GTTAAAATTC
              421 GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC
              481 CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG
              541 AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC
              601 GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA
              661 GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG
              721 AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAAG
              781 TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG
              841 GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT
              901 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAAAC CCTGATAAAT GCTTCAATAA
              961 TATTGAAAAA AGGAAGAGTA TGAGTATTTC ACATTTCCGT GTCGCCCTTA TTCCCTTTTT
             1021 TTGCGGCATT TTGCCTTTCC TGTTTTTGCT CACCCAGAAA CGCTGGGTGA AAGTTAAAAG
             1081 ATGCCTGAAG ATCAGGTTGG GTTGCACGAT GGGGTACATT CGAAACTGGA ATTCCTTCAA
            1141 CAAGGCGGGT TAGAAGAGTG CCT
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]

            2023.08.18

            GNP_filter3_RDBDEP_html_230702.pl