RDB15026 - pEGFP-C1-human RAB3GAP1

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pEGFP-C1-human RAB3GAP1

Expression vector of human RAB3 GTPase activating protein catalytic subunit 1 (RAB3GAP1), fused with N-terminal EGFP, CMV promoter.

Catalog number	RDB15026
Resource name	pEGFP-C1-human RAB3GAP1
Alternative name	KIAA0066
Clone info.	Expression vector of human RAB3 GTPase activating protein catalytic subunit 1 (RAB3GAP1), fused with N-terminal EGFP, CMV promoter.
Comment	Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone	pEGFP-C1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	human RAB3GAP1 cDNA
Reference of insert sequence	AB449877 (DDBJ accession number)
Depositor\|Developer	Fukuda, Mitsunori \|

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human RAB3GAP1

External Database
human RAB3GAP1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)

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提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15026	pEGFP-C1-human RAB3GAP1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15026_A7Egp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 Sequence file: RDB15026_A7Ega.seq
>D04556G2_A7Eg_1_EGFP-C2_C08_08_ABI24.ab1 1 TACCCACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC 61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT ATGGCTGCCG ACAGTGAGCC 121 CGAATCCGAG GTATTTGAGA TCACGGACTT CACCACTGCC TCGGAATGGG AAAGGTTTAT 181 TTCCAAAGTT GAAGAAGTCT TGAATGACTG GAAACTGATT GGAAACTCTT TGGGAAAGCC 241 ACTCGAAAAG GGTATATTTA CTTCTGGCAC ATGGGAAGAG AAATCAGATG AAATTTCCTT 301 TGCTGACTTC AAGTTCTCAG TCACTCATCA TTATCTTGTA CAAGAGTCCA CTGATAAAGA 361 AGGAAAGGAT GAGTTATTAG AGGATGTTGT TCCACAATCT ATGCAAGATT TGCTGGGTAT 421 GAATAATGAC TTTCCTCCAA GAGCACATTG CCTGGTAAGA TGGTATGGGC TACGTGAGTT 481 CGTGGTGATT GCCCCTGCTG CACACAGTGA CGCTGTTCTC AGCGAATCTA AGTGCAACCT 541 TCTTCTGAGT TCTGTTTCTA TTGCCTTGGG AAACACTGGC TGTCAGGTGC CACTCTTTGT 601 GCAAATTCAC CACAAATGGC GAAGAATGTA TGTAGGAGAA TGTCAAGGTC CTGGTGTACG 661 AACTGATTTC GAAATGGTTC ATCTTAGAAA AGTGCCAAAT CAGTACACTC ACTTATCAGG 721 TCTGCTGGAT ATCTTCAAAT CAAAGATTGG ATGTCCTTTA ACTCCATTGC CTCCAGTTAG 781 TATTGCTATT CGATTTACCT ATGTACTTCA AGATTGGCAG CAGTATTTTT GGCCTCAGCA 841 ACCTCCAGAC ATAGATGCCC TTGTAGGAGG AGAAGTTGGA GGCTTGGAGT TTGGCAAGTT 901 ACCATTTGGT GCCTGCGAAG ATCCTATTAG TGAACTCCAT TTAGCTACTA CATGGCCCTC 961 ATCTGACCGA ATGGATCATT GTGGATAATG ATGTTTATTC TGATTTGGGA TCCTATTTCA 1021 AGCTCCACAT TGGTCTGTTA GAGTTTCGAA AAGCTGAGAA TCCTTCAGTG TTTGCCTAGG 1081 TGATTTTTGT CACTTGAATT TTTTCAAAAT TTGCCCGTTC GAAGAGTTCG ACCGGATGGA 1141 ATCTGGACGA ATCTCGGCCA AAT //
Primer: SV40polyA-R Sequence file: RDB15026_A7Egb.seq
>D04556G2_A7Eg_1_SV40polyA_R_A10_01_ABI24.ab1 1 AAAAGTTGGA GTTTTTAAGC AAGTAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA 61 GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA TTCGAAGCTT 121 GGTGCTGGGA AGTCTTTCCG ATGCTGGTTT GATTCCTGAT CACCCAACTG GACAGGGCCC 181 TCTCCAGGAC CTCCCAGGTT CTCCCTGGTA CCTTCCCTCC CTCAGGAGGA GGCAGCACTG 241 TCTCTGAAGC CACCAACGAG TAATGCTAGA AGAATCAGAA GAAGGAAGTA TCTGATGAAA 301 AGGCACCTGC AAGTCTAAAG TCCTCTTTGG TGAGAACACT GTACATCCGC TGAGGCAGAG 361 CTTTGGAGTA GGGAGCAGGG CGCGGCACAG TGGTGCGCAA AATGAATTCC CGGCCAGCAG 421 GGGGTGGGAA GTCTGACACG GAGTTCTGCC TTCTTTCCTC TGGGGAGCCC ATTCTCTTCA 481 ATTCCTCCTC TGGTGGAGTC ATAGCTGCAG CCCTCTGGGC ATTCACAAAC AGCTTGTGAA 541 TGATCCTGCC AGCATGTCCT CTTCCTGCAC CGGTGACTAA CACTTCAGGC TGCTCCAGCA 601 GGCAACTCAC AAACCTTTCA AGATCTTCCT TTTCCTCCTC CTGTTCACAT TTCTCAGTTC 661 CAAACTTGGC TTTTAGTGAC CGAGCTCTGG CAATGAGAGC TTCCACATTA GTAATCTGGT 721 GAATGATTTC TTCCAATTTC TTGTCTTCTG GATTGGGGAA GTGCAAAACT TTACTGGAAT 781 GGGATATTAT CTGCTTTATG ATCTTCTTAA CTGAAGAAAT GTTTTCGAGA CTTTCTTCTT 841 CCTTTACCTT GAGTACAGCT GCATGAATCA CACAAGGTAA CAGGTGCCGA GCAAGGTCTG 901 CAGTTTCTGG ATTGCCAGAT AGTGCAGCAC CTTTTCTGCT TCCCGTGTAT CATCAAAGAG 961 TCTCCCTTTG CCCTTCTAGC AGAAATTGGG CTTTAGCTGT TTCCCAGGCT TCTACCCACA 1021 TATTGCTTTG GAATCATCAT CGGGGCACTC AGGTTCTCAT TCAGCACAAC ATGGCCGTTT 1081 CATCAATCAA CGTCGTGTCA ATATAATTCC CGGGGTGAAT ACCTAACCGT //
Primer: SV40pro_F_V2 Sequence file: RDB15026_A7Egc.seq
>D04556G2_A7Eg_1_SV40pro_F_V2_G11_20_ABI24.ab1 1 GTGCAACCTT TTTTGGAGGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT 61 CGTTTCGCAT GATCTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC 601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGAAATGAC CGACCAAGCG 901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCCTTCTATG AAAGGTTGGG 961 GCTTTCGGAA TCGTTTTCCG GGACGCCCGC CTGGATGATC CTTCCAGCGC GGGATCTCAT 1021 GCCTGGAGTT TCTTTCGGCC CCACCCTAGG GGGGAGGCTA AACTGAAACA CGAGAGACAT 1081 TAACCGGAAG GACCCGCCGC TATGAACGGC AATAAGGACA GAATTAAACG GTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl