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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-human TBC1D6

Expression vector of human TBC1 domain family member 6 (TBC1D6), fused with N-terminal EGFP, CMV promoter.

Catalog number RDB14988
Resource name pEGFP-C1-human TBC1D6
Alternative name FLJ22474/GRTP1
Clone info. Expression vector of human TBC1 domain family member 6 (TBC1D6), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human GRTP1 cDNA
Reference of insert sequence AB449887 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
human GRTP1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。
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          RDB14988 pEGFP-C1-human TBC1D6 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14988_B0Kcp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP 3', insert 5'
          Sequence file: RDB14988_B0Kca.seq check
          1
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert 3'
          Sequence file: RDB14988_B0Kcb.seq check
          1
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, EGFP 5'
          Sequence file: RDB14988_B0Kcc.seq check
          1
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB14988_B0Kcd.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

          2023.04.10

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