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pAcGFP1-N1-MAHS

Expression vector of GFP-fused water bear (Ramazzottius varieornatus) Mitochondrial Abundant Heat Soluble (MAHS).

Catalog number RDB14655
Resource name pAcGFP1-N1-MAHS
Clone info. Expression vector of GFP (AcGFP)-fused water bear (Ramazzottius varieornatus) Mitochondrial Abundant Heat Soluble (MAHS) under the CMV promoter for transient expression in mammalian cells.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pAcGFP1-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus MAHS cDNA
Reference of insert sequence LC002822 (DDBJ accession number)
Depositor|Developer Kunieda, Takekazu |
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Ramazzottius varieornatus MAHS
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            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.（Tanaka, S et al. PLoS One, 10(2): e0118272, 2015)
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            提供条件 利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Tanaka, S et al. PLoS One, 10(2): e0118272, 2015)。
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            RDB14655 pAcGFP1-N1-MAHS Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pAcGFP1-N1-MAHS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14655).

            Reference section:

            Tanaka, S., Tanaka, J., Miwa, Y., Horikawa, D.D., Katayama, T., Arakawa, K., Toyoda, A., Kubo, T., Kunieda, T., Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. PLoS One 10 (2): e0118272 (2015). PMID 25675104. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Tanaka, S., Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. PLoS One 10 (2): e0118272 (2015). PMID 25675104. [link to RRC of NBRP]

            2023.12.13

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