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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Tyrosinase-FLAG

Expression vector of mouse Tyrosinase with C-terminal FLAG-tag under the control of the EF1 alpha promoter.

Catalog number RDB14635
Resource name pEF-Tyrosinase-FLAG
Clone info. Expression vector of mouse Tyrosinase with C-terminal FLAG-tag under the control of the EF1 alpha promoter.
Comment Please refer Kanno, E. et al. Traffic, 11 (4): 491-507, 2010 (PMID 20070612) for pEF-FLAG vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone pEF-FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Tyr cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
Mouse Tyr

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14635 pEF-Tyrosinase-FLAG DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0070e (English text)
          Featured content Dnaconda's recommendation EXR0070j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14635_A7C1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB14635_A7C1a.seq check
          >D04384A4_A7C1_2_EF1a-C_F_C03_09_ABI24.ab1
              1 TTATTTGCAT TGTCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT
             61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGGAATT CTCTAGAGCG GCCGCTCTAG
            121 AACTAGTGGA TCCATGTTCT TGGCTGTTTT GTATTGCCTT CTGTGGAGTT TCCAGATCTC
            181 TGATGGCCAT TTTCCTCGAG CCTGTGCCTC CTCTAAGAAC TTGTTGGCAA AAGAATGCTG
            241 CCCACCATGG ATGGGTGATG GGAGTCCCTG CGGCCAGCTT TCAGGCAGAG GTTCCTGCCA
            301 GGATATCCTT CTGTCCAGTG CACCATCTGG ACCTCAGTTC CCCTTCAAAG GGGTGGATGA
            361 CCGTGAGTCC TGGCCCTCTG TGTTTTATAA TAGGACCTGC CAGTGCTCAG GCAACTTCAT
            421 GGGTTTCAAC TGCGGAAACT GTAAGTTTGG ATTTGGGGGC CCAAATTGTA CAGAGAAGCG
            481 AGTCTTGATT AGAAGAAACA TTTTTGATTT GAGTGTCTCC GAAAAGAATA AGTTCTTTTC
            541 TTACCTCACT TTAGCAAAAC ATACTATCAG CTCAGTCTAT GTCATCCCCA CAGGCACCTA
            601 TGGCCAAATG AACAATGGGT CAACACCCAT GTTTAATGAT ATCAACATCT ACGACCTCTT
            661 TGTATGGATG CATTACTATG TGTCAAGGGA CACACTGCTT GGGGGCTCTG AAATATGGAG
            721 GGACATTGAT TTTGCCCATG AAGCACCAGG GTTTCTGCCT TGGCACAGAC TTTTCTTGTT
            781 ATTGTGGGAA CAAGAAATTC GAGAACTAAC TGGGGATGAG AACTTCACTG TTCCATACTG
            841 GGATTGGAGA GATGCAGAAA ACTGTGACAT TTGCACAGAT GAGTACTTGG GAGGTCGTCA
            901 CCCTGAAAAT CCTAACTTAC TCAGCCCAGC ATCCTTCTTC TCCTCCTGGC AGATCATTTG
            961 TAGCAGATCA GAAGAGTATA ATAGCCATCA GGTTTTATGC GATGGAAACA CCCTGAGGGA
           1021 CCACTATTTA CGTAAATCCT GGAAAACCAT GACAAAAGCC CAAAACCCCC CAGGCTCCAT
           1081 CCTCCAGCAG ATGTGATTTG GTCCTGGATT GACCAGTATG AATCTGGAAT TCAAAAGTGG
          //
          Primer: hGCSF_R
          Sequence file: RDB14635_A7C1b.seq check
          >D04384A4_A7C1_2_hGCSF_R_D03_12_ABI24.ab1
              1 GTGGGGAAAA GGGATCTCTC AAGGGTCCCA GGGTGGGGAC CCTCACTCTA GAGCGGCCGC
             61 CTACTTGTCG TCATCGTCCT TGTAGTCCCG CGGGGTACCC AGATGGCTCT GATACAGCAA
            121 GCTGTGGTAG TCGTCTTTGT CCATGAGGAG TGGCTGCCTT TCCTCCTGGG GTTGCTTCTT
            181 CTTCTTCTTC TTCTGAAGGC ATAGCCTACT GCTAAGCCCA GAGAGAGCTG CAGCAATAAC
            241 AGCTCCCACC AGTGCTGCCC CAAGAAGCCA TGGCCAGATA CGACTGGCTT GTTCCAAGTA
            301 AGGCTCAATA TAATTTCTGT AAAAGCCTGG ATCTGACTCT TGGAGGTAGC TGTAGTCATA
            361 TCCCAGATCC TTGGATGTTA TGAAGAAATC ACCATTTCTA TAGAGCGGTA TGAAAGGAAC
            421 CATGTAAGAG TCTCTGTTAT GGCCGATAGG TGCATTGGCT TCTGGGTAAA CTTCCAAAAG
            481 AGGGCGGTGC CTTCGCAGCC ATTGTTCAAA AATACTGTCC ACAAAAGCAT GGTGAAGAAG
            541 AAAAATGGGA TCGTTGGCCG ATCCCTGTAC TTGGGACATT GTTCCATTCA TAAAGATATG
            601 TAAGGCATTG TGCATGCTAC TTTGAGAAGG ATCTGCTATC CCTGTGAGTG GACTGGCAAA
            661 TCCTTCCAGT GTGTTTCTAA AGCTGAAATT GGCAGTTCTA TCCATTGATC CAGATTCATA
            721 CTGGGTCAAA CTCAGACAAA ATTCCACATC TGCTGAAGAT GGGAGCCTGG GGGTTTTGGC
            781 TTTGTCATGG TTTCCAGGAT TACGTAATAG TGGTCCCTCA GTGTTCCATC GCATAAAACC
            841 TGATGGCTAT TATACTCTTC TGATCTGCTA CAAATGATCT GCCAGGAGGA GAAGAAGGAT
            901 GCTGGGCTGA GTAAGTTAGG ATTTTCAGGG TGACGACCTC CCAAGTACTC ATCTGTGCAA
            961 ATGTCACAGA TTTCTGCATC TCTCCCAATC CCAGTATGGA AACAGTGAAG TTTCTCATCC
           1021 CCAGTTTAGT TCTTCGAAAT TTCTTGTTCC CACCAATAAA CAAGAAAAAG TCTGTGCTAG
           1081 GCAGAAACCT GGGTGCTCAT GGCCAAAATT CCAATGTTCC CTCCATATTT TCAGAAAGCC
          1141 CCCCCCCGAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl